Strain Name:
C57BL/6J-MtgxR4958Btlr/Mmmh
Stock Number:
042555-MU
Citation ID:
RRID:MMRRC_042555-MU
Other Names:
R4958 (G1), C57BL/6J-MtgxR4958Btlr
Major Collection:

Gene Information

Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, 4932417H02Rik, Rap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74370
Homologene: 80210
Derl2
Name: Der1-like domain family, member 2
Synonyms: CGI-101, F-lana, Flana, Derlin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 116891
Homologene: 9356
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Usp5
Name: ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms: Ucht
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22225
Homologene: 55758
Sulf1
Name: sulfatase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240725
Homologene: 49408
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: trithorax Drosophila, HTRX1, ALL-1, All1, Cxxc7, Mll, Mll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Atf7ip
Name: activating transcription factor 7 interacting protein
Synonyms: ATFa-associated Modulator, AM, 2610204M12Rik, Mcaf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54343
Homologene: 10051
Dennd4c
Name: DENN/MADD domain containing 4C
Synonyms: 1700065A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329877
Homologene: 23057
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: MAP1C, dynein heavy chain, retrograde transport, Dnec1, Loa, 9930018I23Rik, Dnchc1, Swl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Ep300
Name: E1A binding protein p300
Synonyms: p300, KAT3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 328572
HGNC: HGNC:3373
Homologene: 1094
Tshr
Name: thyroid stimulating hormone receptor
Synonyms: hyt, pet, hypothroid
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 22095
Homologene: 315
Ereg
Name: epiregulin
Synonyms: EPR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13874
HGNC: HGNC:3443
Homologene: 1097
Adrm1
Name: adhesion regulating molecule 1
Synonyms: ARM-1, 2510006J17Rik, Gp110, 1110063P18Rik, Rpn13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56436
Homologene: 10513
Arel1
Name: apoptosis resistant E3 ubiquitin protein ligase 1
Synonyms: 1110018G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68497
Homologene: 8885
Syt13
Name: synaptotagmin XIII
Synonyms: 5730409J20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 80976
Homologene: 10823
Tjp1
Name: tight junction protein 1
Synonyms: ZO-1, ZO1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 21872
Homologene: 2445
Eef1a1
Name: eukaryotic translation elongation factor 1 alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13627
HGNC: HGNC:3189
Homologene: 100799
Nsd2
Name: nuclear receptor binding SET domain protein 2
Synonyms: 5830445G22Rik, Whsc1l, 9430010A17Rik, D030027O06Rik, C130020C13Rik, D930023B08Rik, Whsc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 107823
Homologene: 26175
Carm1
Name: coactivator-associated arginine methyltransferase 1
Synonyms: Prmt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 59035
Homologene: 10990
Pbrm1
Name: polybromo 1
Synonyms: BAF180, 2610016F04Rik, 2310032M22Rik, Pb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66923
Homologene: 10044
Map3k5
Name: mitogen-activated protein kinase kinase kinase 5
Synonyms: ASK1, Mekk5, 7420452D20Rik, ASK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 26408
HGNC: HGNC:6857
Homologene: 38114
Slco1a6
Name: solute carrier organic anion transporter family, member 1a6
Synonyms: organic anion-transporting polypeptide, Oatp-5, 4930422F19Rik, Slc21a13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 28254
Homologene: 23416
Ankrd7
Name: ankyrin repeat domain 7
Synonyms: 4930532L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 75196
Homologene: 35506
Myh2
Name: myosin, heavy polypeptide 2, skeletal muscle, adult
Synonyms: MyHC-IIa, MHC2A, Myhs-f1, Myhs-f, Myhsf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17882
HGNC: HGNC:7572
Homologene: 23019
Mfsd6
Name: major facilitator superfamily domain containing 6
Synonyms: 9630025I22Rik, MMR2, 2210010L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98682
Homologene: 9784
Atp1a4
Name: ATPase, Na+/K+ transporting, alpha 4 polypeptide
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 27222
Homologene: 113769
Rab11fip1
Name: RAB11 family interacting protein 1 (class I)
Synonyms: 2010200K21Rik, 4833414G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 75767
Homologene: 11853
Serac1
Name: serine active site containing 1
Synonyms: 4930511N22Rik, D17Ertd141e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 321007
Homologene: 41900
Cdh2
Name: cadherin 2
Synonyms: N-cadherin, Ncad, N-CAD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12558
HGNC: HGNC:1759
Homologene: 20424
Zswim8
Name: zinc finger SWIM-type containing 8
Synonyms: 4832404P21Rik, 2310021P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 268721
VEGA: 14
Homologene: 34313
Mboat1
Name: membrane bound O-acyltransferase domain containing 1
Synonyms: 9130215M02Rik, Oact1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218121
Homologene: 57467
Olfr1099
Name: olfactory receptor 1099
Synonyms: GA_x6K02T2Q125-48446067-48445129, MOR206-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258764
Homologene: 107003
Noxo1
Name: NADPH oxidase organizer 1
Synonyms: 2310034C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 71893
Homologene: 12418
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Plscr4
Name: phospholipid scramblase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235527
Homologene: 23217
Olfr667
Name: olfactory receptor 667
Synonyms: GA_x6K02T2PBJ9-7546146-7545166, MOR34-2, MOR34-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259062
Homologene: 121559
Olfr633
Name: olfactory receptor 633
Synonyms: GA_x6K02T2PBJ9-6681230-6682168, MOR12-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258351
Homologene: 27136
Cdk3
Name: cyclin-dependent kinase 3
Synonyms: Cdk3-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69681
HGNC: HGNC:1772
Olfr1336
Name: olfactory receptor 1336
Synonyms: GA_x6K02T2QGBW-3190370-3191314, MOR103-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258917
Homologene: 37007
Gm4950
Name: predicted pseudogene 4950
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240289
VEGA: 18
Znrf4
Name: zinc and ring finger 4
Synonyms: spzn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20834
VEGA: 17
Homologene: 7959
Pla2g1b
Name: phospholipase A2, group IB, pancreas
Synonyms: Pla2a, sPLA2IB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18778
HGNC: HGNC:9030
Homologene: 715
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 12,796,910 bp
  • T to C, chromosome 1 at 52,661,024 bp
  • T to A, chromosome 1 at 172,231,151 bp
  • T to C, chromosome 2 at 86,959,105 bp
  • G to A, chromosome 2 at 92,953,449 bp
  • G to A, chromosome 2 at 180,175,617 bp
  • A to G, chromosome 4 at 86,781,679 bp
  • G to T, chromosome 4 at 123,475,364 bp
  • T to A, chromosome 5 at 33,892,022 bp
  • T to C, chromosome 5 at 91,090,111 bp
  • T to A, chromosome 5 at 115,470,826 bp
  • A to G, chromosome 6 at 18,866,723 bp
  • C to G, chromosome 6 at 124,822,630 bp
  • C to T, chromosome 6 at 136,606,810 bp
  • C to A, chromosome 6 at 142,145,705 bp
  • G to A, chromosome 7 at 6,461,058 bp
  • G to A, chromosome 7 at 65,336,102 bp
  • T to C, chromosome 7 at 103,946,601 bp
  • T to C, chromosome 7 at 104,916,461 bp
  • G to T, chromosome 8 at 27,154,813 bp
  • A to G, chromosome 9 at 21,583,532 bp
  • A to T, chromosome 9 at 44,848,467 bp
  • A to G, chromosome 9 at 78,479,582 bp
  • A to G, chromosome 9 at 92,484,761 bp
  • A to T, chromosome 10 at 20,023,789 bp
  • A to T, chromosome 10 at 76,723,505 bp
  • G to T, chromosome 11 at 60,711,435 bp
  • A to G, chromosome 11 at 67,192,959 bp
  • A to G, chromosome 11 at 71,014,637 bp
  • A to G, chromosome 11 at 116,217,143 bp
  • G to A, chromosome 11 at 119,857,391 bp
  • T to C, chromosome 12 at 84,926,304 bp
  • A to G, chromosome 12 at 91,538,187 bp
  • C to A, chromosome 12 at 110,658,126 bp
  • A to G, chromosome 13 at 13,635,463 bp
  • T to C, chromosome 13 at 30,224,393 bp
  • T to A, chromosome 14 at 20,713,465 bp
  • T to C, chromosome 14 at 31,074,827 bp
  • A to G, chromosome 15 at 81,601,407 bp
  • A to T, chromosome 17 at 6,069,382 bp
  • T to C, chromosome 17 at 24,700,274 bp
  • A to G, chromosome 17 at 56,511,701 bp
  • T to G, chromosome 18 at 16,627,565 bp
  • T to C, chromosome 18 at 51,865,569 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4958 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042555-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.