Strain Name:
C57BL/6J-MtgxR5019Btlr/Mmmh
Stock Number:
042610-MU
Citation ID:
RRID:MMRRC_042610-MU
Other Names:
R5019 (G1), C57BL/6J-MtgxR5019Btlr
Major Collection:

Strain Information

Mfap2
Name: microfibrillar-associated protein 2
Synonyms: Magp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17150
HGNC: HGNC:7033
Homologene: 1801
Tfap2b
Name: transcription factor AP-2 beta
Synonyms: AP-2(beta), E130018K07Rik, Tcfap2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21419
Homologene: 20688
Kdm4c
Name: lysine (K)-specific demethylase 4C
Synonyms: 2410141F18Rik, Jmjd2c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 76804
Homologene: 41004
Zfp809
Name: zinc finger protein 809
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235047
VEGA: 9
Ahcyl2
Name: S-adenosylhomocysteine hydrolase-like 2
Synonyms: 4631427C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74340
Homologene: 69337
Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: 53BP1, p53BP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 27223
Homologene: 4137
Miga1
Name: mitoguardin 1
Synonyms: C030011O14Rik, Mita1, Fam73a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 215708
Homologene: 18369
Trim27
Name: tripartite motif-containing 27
Synonyms: Rfp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19720
HGNC: HGNC:9975
Homologene: 111010
Pex1
Name: peroxisomal biogenesis factor 1
Synonyms: ZWS1, 5430414H02Rik, E330005K07Rik, peroxisome biogenesis factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71382
HGNC: HGNC:8850
Homologene: 27006
Add3
Name: adducin 3 (gamma)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 27360
VEGA: 19
HGNC: HGNC:245
Homologene: 40893
Carm1
Name: coactivator-associated arginine methyltransferase 1
Synonyms: Prmt4, m9Bei
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 59035
Homologene: 10990
Psmd5
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
Synonyms: S5b, 1500032A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66998
HGNC: HGNC:9563
Homologene: 37999
Pigg
Name: phosphatidylinositol glycan anchor biosynthesis, class G
Synonyms: Gpi7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 433931
Homologene: 39421
Zfp790
Name: zinc finger protein 790
Synonyms: 6330581L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233056
Homologene: 51695
Plcb2
Name: phospholipase C, beta 2
Synonyms: B230205M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18796
HGNC: HGNC:9055
Homologene: 20957
Abca9
Name: ATP-binding cassette, sub-family A (ABC1), member 9
Synonyms: D630040K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217262
HGNC: HGNC:39
Homologene: 33332
Ttc28
Name: tetratricopeptide repeat domain 28
Synonyms: 2310015L07Rik, TPRBK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 209683
Homologene: 41023
Xkr6
Name: X-linked Kx blood group related 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219149
Homologene: 18287
Zfp618
Name: zinc finger protein 618
Synonyms: D430033D05Rik, 2810040O04Rik, 2810031P15Rik, Nedd10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72701
Homologene: 18975
Skint8
Name: selection and upkeep of intraepithelial T cells 8
Synonyms: OTTMUSG00000009475
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 639774
Homologene: 106613
Ip6k2
Name: inositol hexaphosphate kinase 2
Synonyms: 1500005N04Rik, Ihpk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76500
Homologene: 56929
Klra5
Name: killer cell lectin-like receptor, subfamily A, member 5
Synonyms: Ly49e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16636
Homologene: 110821
Xkr5
Name: X-linked Kx blood group related 5
Synonyms: 5430438H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319581
Homologene: 52219
Or2l5
Name: olfactory receptor family 2 subfamily L member 5
Synonyms: GA_x54KRFPKG5P-15963726-15962788, MOR272-1, Olfr167
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258937
Homologene: 78689
Adamts17
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 17
Synonyms: AU023434
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233332
Homologene: 16373
Asb3
Name: ankyrin repeat and SOCS box-containing 3
Synonyms: 2400011J03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 65257
Homologene: 9391
Or4d2b
Name: olfactory receptor family 4 subfamily D member 2B
Synonyms: GA_x6K02T2PAEV-9536824-9535889, MOR240-3, Olfr462
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258406
HGNC: HGNC:8294
Homologene: 64870
Nalf1
Name: NALCN channel auxiliary factor 1
Synonyms: Fam155a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270028
Homologene: 83472
Pate12
Name: prostate and testis expressed 12
Synonyms: Gm7257
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 639025
Homologene: 86450
Pcdhgb6
Name: protocadherin gamma subfamily B, 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93703
Homologene: 49573
Mars2
Name: methionine-tRNA synthetase 2 (mitochondrial)
Synonyms: C730026E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 212679
Homologene: 6009
Gm26954
Name: predicted gene, 26954
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 19,226,442 bp
  • T to C, chromosome 1 at 55,237,309 bp
  • A to G, chromosome 2 at 34,865,953 bp
  • A to T, chromosome 2 at 118,712,136 bp
  • A to T, chromosome 2 at 121,270,319 bp
  • A to C, chromosome 3 at 152,322,461 bp
  • T to C, chromosome 4 at 63,103,552 bp
  • T to C, chromosome 4 at 74,343,535 bp
  • T to C, chromosome 4 at 111,928,648 bp
  • A to T, chromosome 4 at 141,015,258 bp
  • T to C, chromosome 5 at 3,622,331 bp
  • A to G, chromosome 5 at 14,714,367 bp
  • A to G, chromosome 5 at 108,332,149 bp
  • T to A, chromosome 5 at 111,102,064 bp
  • A to T, chromosome 6 at 29,859,739 bp
  • T to C, chromosome 6 at 129,899,389 bp
  • C to T, chromosome 7 at 29,829,767 bp
  • T to A, chromosome 7 at 67,062,070 bp
  • A to G, chromosome 8 at 9,770,240 bp
  • T to C, chromosome 8 at 18,942,110 bp
  • T to C, chromosome 9 at 21,579,511 bp
  • A to G, chromosome 9 at 22,237,702 bp
  • C to A, chromosome 9 at 36,431,902 bp
  • T to A, chromosome 9 at 108,797,746 bp
  • A to T, chromosome 11 at 31,081,415 bp
  • A to G, chromosome 11 at 87,888,975 bp
  • C to A, chromosome 11 at 110,165,934 bp
  • T to C, chromosome 13 at 21,189,964 bp
  • T to G, chromosome 14 at 63,819,066 bp
  • A to T, chromosome 16 at 19,515,535 bp
  • A to G, chromosome 18 at 37,742,941 bp
  • A to G, chromosome 19 at 53,242,571 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5019 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042610-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.