Strain Name:
C57BL/6J-MtgxR5031Btlr/Mmmh
Stock Number:
042622-MU
Citation ID:
RRID:MMRRC_042622-MU
Other Names:
R5031 (G1), C57BL/6J-MtgxR5031Btlr
Major Collection:

Strain Information

Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: Nos-1, nNOS, 2310005C01Rik, bNOS, NO
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Dock1
Name: dedicator of cytokinesis 1
Synonyms: 9130006G06Rik, Dock180, D630004B07Rik, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Cdca2
Name: cell division cycle associated 2
Synonyms: 2610311M19Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 108912
Homologene: 18444
Virma
Name: vir like m6A methyltransferase associated
Synonyms: 1110037F02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66185
Homologene: 41043
Atr
Name: ataxia telangiectasia and Rad3 related
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245000
HGNC: HGNC:882
Homologene: 96916
Trappc12
Name: trafficking protein particle complex 12
Synonyms: D930014A20Rik, CGI-87, Ttc15
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217449
VEGA: 12
Homologene: 34805
Relch
Name: RAB11 binding and LisH domain, coiled-coil and HEAT repeat containing
Synonyms: 2310035C23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227446
Homologene: 10834
Cct8
Name: chaperonin containing TCP1 subunit 8
Synonyms: Cctq, Tcpq
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12469
HGNC: HGNC:1623
Homologene: 4802
Nabp2
Name: nucleic acid binding protein 2
Synonyms: SSB1, Nabp2, Obfc2b, 2610036N15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 69917
Homologene: 11446
Qrich1
Name: glutamine-rich 1
Synonyms: 2610028H07Rik, b2b2404Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69232
Homologene: 9803
Mrgpra1
Name: MAS-related GPR, member A1
Synonyms: MrgA1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233221
Homologene: 79615
Lrrk2
Name: leucine-rich repeat kinase 2
Synonyms: cI-46, LOC381026, 9330188B09Rik, D630001M17Rik, 4921513O20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66725
Homologene: 18982
Epg5
Name: ectopic P-granules 5 autophagy tethering factor
Synonyms: 5430411K18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 100502841
VEGA: 18
Homologene: 14575
Magel2
Name: MAGE family member L2
Synonyms: Mage-l2, ns7, nM15, NDNL1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27385
HGNC: HGNC:6814
Homologene: 8460
AU021092
Name: expressed sequence AU021092
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239691
Homologene: 17587
Dmkn
Name: dermokine
Synonyms: dermokine, Dmkn, 1110014F24Rik, sk89, sk30, cI-36
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73712
Homologene: 136295
Mettl16
Name: methyltransferase 16, N6-methyladenosine
Synonyms: 2810013M15Rik, Mett10d, 2610100D03Rik, A830095F14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67493
Homologene: 34653
Ints2
Name: integrator complex subunit 2
Synonyms: 2810417D08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70422
Homologene: 10801
Baz2b
Name: bromodomain adjacent to zinc finger domain, 2B
Synonyms: D2Ertd794e, 5830435C13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 407823
HGNC: HGNC:963
Homologene: 8394
Tbc1d32
Name: TBC1 domain family, member 32
Synonyms: b2b2284Clo, Bromi, D630037F22Rik, C6orf170
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 544696
VEGA: 10
Homologene: 51889
Rspo3
Name: R-spondin 3
Synonyms: Thsd2, Cristin1, 2810459H04Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72780
VEGA: 10
Homologene: 12484
Ank1
Name: ankyrin 1, erythroid
Synonyms: pale, Ank-1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11733
HGNC: HGNC:492
Homologene: 55427
Abca13
Name: ATP-binding cassette, sub-family A member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268379
Homologene: 27991
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 545370
Homologene: 23741
Gsap
Name: gamma-secretase activating protein
Synonyms: Pion, A530088I07Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 212167
Homologene: 45504
Irs1
Name: insulin receptor substrate 1
Synonyms: IRS-1, G972R
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16367
HGNC: HGNC:6125
Homologene: 4049
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: Heatr7b1, OTTMUSG00000020804, ENSMUSG00000044873
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239420
Homologene: 65982
Acnat2
Name: acyl-coenzyme A amino acid N-acyltransferase 2
Synonyms: C730036D15Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 209186
Homologene: 28287
Spn
Name: sialophorin
Synonyms: Galgp, leukosialin, 3E8 antigen, A630014B01Rik, Cd43, Ly48, Ly-48
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20737
Homologene: 36108
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 214321
Homologene: 86950
Trpm8
Name: transient receptor potential cation channel, subfamily M, member 8
Synonyms: CMR1, TRPP8, Trp-p8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 171382
Homologene: 23433
Vmn1r228
Name: vomeronasal 1 receptor 228
Synonyms: V1re3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 171226
Homologene: 74320
Scml4
Name: Scm polycomb group protein like 4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 268297
Homologene: 18262
Zfp521
Name: zinc finger protein 521
Synonyms: B930086A16Rik, Evi3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225207
VEGA: 18
Homologene: 9151
Mmut
Name: methylmalonyl-Coenzyme A mutase
Synonyms: Mut, D230010K02Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17850
VEGA: 17
HGNC: HGNC:7526
Homologene: 20097
Tcaf3
Name: TRPM8 channel-associated factor 3
Synonyms: Eapa2, Fam115e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 403088
Homologene: 74374
Or10ag53
Name: olfactory receptor family 10 subfamily AG member 53
Synonyms: Olfr1530-ps1, MOR264-20, Olfr1115, GA_x6K02T2Q125-48736906-48737886, MOR273-4P, MOR273-4P
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258294
Homologene: 27114
Mvp
Name: major vault protein
Synonyms: LRP, VAULT1, 2310009M24Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 78388
HGNC: HGNC:7531
Homologene: 3752
Kyat1
Name: kynurenine aminotransferase 1
Synonyms: cytoplasmic (glutamine transaminase K, kyneurenine aminotransferase), Ccbl1, 2010009K05Rik, Kat1, KATI
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70266
HGNC: HGNC:1564
Homologene: 37872
Ifitm5
Name: interferon induced transmembrane protein 5
Synonyms: Bril, 1110003J06Rik, Hrmp1, fragilis4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73835
Homologene: 14210
Sult1d1
Name: sulfotransferase family 1D, member 1
Synonyms: tyrosine-ester sulfotransferase, Sultn, 5033411P13Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53315
Homologene: 124464
Arhgef19
Name: Rho guanine nucleotide exchange factor 19
Synonyms: WGEF, 6430573B13Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 213649
Homologene: 17710
Or9g4b
Name: olfactory receptor family 9 subfamily G member 4B
Synonyms: Olfr1015, MOR213-3, GA_x6K02T2Q125-47264151-47265089
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258564
Homologene: 115499
Klhl29
Name: kelch-like 29
Synonyms: Kbtbd9, A230106N14Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 208439
VEGA: 12
Homologene: 66272
Hectd2
Name: HECT domain E3 ubiquitin protein ligase 2
Synonyms: A630025O09Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226098
Homologene: 6280
Tram1l1
Name: translocation associated membrane protein 1-like 1
Synonyms: A830091N21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229801
Homologene: 17142
Zfp583
Name: zinc finger protein 583
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 213011
Homologene: 65048
Rab17
Name: RAB17, member RAS oncogene family
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19329
Homologene: 7502
Trav6d-4
Name: T cell receptor alpha variable 6D-4
Synonyms: LOC277146, LOC193570
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 277146
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 82,286,967 bp
  • GCCC to GC, chromosome 1 at 88,232,257 bp
  • A to G, chromosome 1 at 88,348,188 bp
  • A to T, chromosome 1 at 90,960,138 bp
  • A to G, chromosome 1 at 105,664,514 bp
  • A to G, chromosome 1 at 150,588,257 bp
  • A to G, chromosome 2 at 30,188,090 bp
  • T to C, chromosome 2 at 59,912,807 bp
  • T to A, chromosome 2 at 85,785,718 bp
  • C to A, chromosome 2 at 87,252,082 bp
  • T to A, chromosome 3 at 124,321,644 bp
  • T to A, chromosome 4 at 11,542,116 bp
  • T to C, chromosome 4 at 49,380,631 bp
  • A to G, chromosome 4 at 141,250,810 bp
  • G to A, chromosome 5 at 21,242,826 bp
  • T to A, chromosome 5 at 87,559,844 bp
  • C to T, chromosome 5 at 117,879,313 bp
  • A to G, chromosome 6 at 42,596,933 bp
  • T to A, chromosome 7 at 6,317,398 bp
  • A to G, chromosome 7 at 30,764,236 bp
  • A to T, chromosome 7 at 47,335,237 bp
  • T to C, chromosome 7 at 62,380,104 bp
  • A to C, chromosome 7 at 126,993,616 bp
  • G to T, chromosome 7 at 127,137,230 bp
  • A to G, chromosome 7 at 135,152,246 bp
  • G to A, chromosome 7 at 140,950,104 bp
  • C to T, chromosome 8 at 23,099,680 bp
  • A to G, chromosome 9 at 95,865,702 bp
  • C to T, chromosome 9 at 99,071,408 bp
  • C to T, chromosome 9 at 108,541,736 bp
  • A to T, chromosome 10 at 29,506,447 bp
  • T to C, chromosome 10 at 42,955,986 bp
  • C to A, chromosome 10 at 56,123,531 bp
  • G to A, chromosome 10 at 128,409,628 bp
  • A to T, chromosome 11 at 9,297,678 bp
  • A to T, chromosome 11 at 74,802,999 bp
  • G to A, chromosome 11 at 86,256,200 bp
  • C to T, chromosome 12 at 5,091,334 bp
  • T to A, chromosome 12 at 28,692,513 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • A to C, chromosome 14 at 52,753,599 bp
  • T to A, chromosome 14 at 67,713,153 bp
  • A to T, chromosome 15 at 47,659,192 bp
  • A to T, chromosome 15 at 91,700,619 bp
  • T to C, chromosome 16 at 5,212,604 bp
  • C to T, chromosome 16 at 87,487,538 bp
  • T to A, chromosome 17 at 20,776,681 bp
  • T to C, chromosome 17 at 40,938,827 bp
  • T to A, chromosome 18 at 13,844,273 bp
  • G to A, chromosome 18 at 78,028,948 bp
  • A to G, chromosome 19 at 36,599,604 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5031 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042622-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.