Strain Name:
C57BL/6J-MtgxR5099Btlr/Mmmh
Stock Number:
042688-MU
Citation ID:
RRID:MMRRC_042688-MU
Other Names:
R5099 (G1), C57BL/6J-MtgxR5099Btlr
Major Collection:

Strain Information

Gsr
Name: glutathione reductase
Synonyms: Gr-1, Gr1, D8Ertd238e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14782
HGNC: HGNC:4623
Homologene: 531
Tyw5
Name: tRNA-yW synthesizing protein 5
Synonyms: 1110034B05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68736
Homologene: 72085
Unk
Name: unkempt family zinc finger
Synonyms: B230379M23Rik, Zc3h5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217331
Homologene: 18194
Lpin2
Name: lipin 2
Synonyms: 2610511G02Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 64898
Homologene: 8769
Dlx6
Name: distal-less homeobox 6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13396
HGNC: HGNC:2919
Homologene: 87855
Ufl1
Name: UFM1 specific ligase 1
Synonyms: Rcad, Maxer, 1810074P20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67490
Homologene: 9093
Mff
Name: mitochondrial fission factor
Synonyms: 5230400G24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75734
Homologene: 87000
Tox
Name: thymocyte selection-associated high mobility group box
Synonyms: 1700007F02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 252838
Homologene: 8822
Bdkrb1
Name: bradykinin receptor, beta 1
Synonyms: kinin B1, B1R
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 12061
HGNC: HGNC:1029
Homologene: 570
Dcdc2a
Name: doublecortin domain containing 2a
Synonyms: RU2, Dcdc2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 195208
Homologene: 9483
Sp2
Name: Sp2 transcription factor
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78912
Homologene: 2340
Mecom
Name: MDS1 and EVI1 complex locus
Synonyms: ZNFPR1B1, Prdm3, MDS1-EVI1, Evi-1, D630039M04Rik, Jbo, Evi1, Mds1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14013
HGNC: HGNC:3498
Homologene: 21086
Strbp
Name: spermatid perinuclear RNA binding protein
Synonyms: Spnr, 6430510M02Rik, C230082I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20744
Homologene: 7548
Scn1a
Name: sodium channel, voltage-gated, type I, alpha
Synonyms: Nav1.1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20265
Homologene: 21375
Rpgrip1l
Name: Rpgrip1-like
Synonyms: Ftm, 1700047E16Rik, fantom, Nphp8
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244585
Homologene: 18296
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Trgv5
Name: T cell receptor gamma, variable 5
Synonyms: Tcrg-V5
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21639
Rin2
Name: Ras and Rab interactor 2
Synonyms: RASSF4, 4632403N06Rik, 2010003K16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74030
Homologene: 32430
Jakmip2
Name: janus kinase and microtubule interacting protein 2
Synonyms: 6430702L21Rik, D930046L20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 76217
VEGA: 18
Homologene: 8866
Ssu2
Name: ssu-2 homolog
Synonyms: D630042P16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243612
Homologene: 136556
Arsa
Name: arylsulfatase A
Synonyms: As-2, ASA, As2, AS-A
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11883
HGNC: HGNC:713
Homologene: 20138
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 214321
Homologene: 86950
Ift140
Name: intraflagellar transport 140
Synonyms: Wdtc2, Tce5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106633
Homologene: 40979
Or5p5
Name: olfactory receptor family 5 subfamily P member 5
Synonyms: GA_x6K02T2PBJ9-10144091-10145011, MOR204-33P, Olfr467
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 257919
Homologene: 81542
Ccdc178
Name: coiled coil domain containing 178
Synonyms: 4921528I01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 70950
VEGA: 18
Homologene: 12373
Ms4a20
Name: membrane-spanning 4-domains, subfamily A, member 20
Synonyms: 1700017D01Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 69369
Vmn1r60
Name: vomeronasal 1 receptor 60
Synonyms: Gm7184
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 636697
Homologene: 41799
Ube2q2
Name: ubiquitin-conjugating enzyme E2Q family member 2
Synonyms: 3010021M21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 109161
Homologene: 45455
Ppm1n
Name: protein phosphatase, Mg2+/Mn2+ dependent, 1N (putative)
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232941
Homologene: 58958
Vmn1r81
Name: vomeronasal 1 receptor 81
Synonyms: V1rg9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 171244
Homologene: 74327
Gbp9
Name: guanylate-binding protein 9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 236573
Homologene: 128731
Slfn3
Name: schlafen 3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20557
Homologene: 133236
Ceacam5
Name: CEA cell adhesion molecule 5
Synonyms: 1600029H12Rik, Psg30
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73250
HGNC: HGNC:1819
Homologene: 115938
Prr22
Name: proline rich 22
Synonyms: LOC224908, Gm546
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 100504446
Homologene: 51921
Or5ac24
Name: olfactory receptor family 5 subfamily AC member 24
Synonyms: GA_x54KRFPKG5P-55560552-55559632, MOR182-4, Olfr206
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258993
Homologene: 74262
Tmem176b
Name: transmembrane protein 176B
Synonyms: Clast1, Lr8, 1810009M01Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 65963
Homologene: 8521
Tada2b
Name: transcriptional adaptor 2B
Synonyms: LOC231151
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231151
Homologene: 51007
Ptprv
Name: protein tyrosine phosphatase receptor type V
Synonyms: mOST-PTP, Esp, OST-PTP, OST
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13924
Homologene: 7306
Or8k36-ps1
Name: olfactory receptor family 8 subfamily K member 36, pseudogene 1
Synonyms: GA_x6K02T2Q125-48092599-48091658, MOR191-3, Olfr1083-ps
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 404326
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 57,388,705 bp
  • C to T, chromosome 1 at 82,750,471 bp
  • T to C, chromosome 1 at 135,118,854 bp
  • T to C, chromosome 2 at 37,603,018 bp
  • C to T, chromosome 2 at 52,195,448 bp
  • A to G, chromosome 2 at 66,277,801 bp
  • A to T, chromosome 2 at 86,607,216 bp
  • G to A, chromosome 2 at 145,878,901 bp
  • A to G, chromosome 3 at 29,985,316 bp
  • G to T, chromosome 4 at 6,688,958 bp
  • C to T, chromosome 4 at 25,275,914 bp
  • A to T, chromosome 5 at 36,476,400 bp
  • A to T, chromosome 5 at 105,094,513 bp
  • C to A, chromosome 6 at 6,865,143 bp
  • T to C, chromosome 6 at 48,834,529 bp
  • A to G, chromosome 6 at 112,359,624 bp
  • A to T, chromosome 7 at 5,544,817 bp
  • A to G, chromosome 7 at 12,260,321 bp
  • T to C, chromosome 7 at 17,745,588 bp
  • A to T, chromosome 7 at 19,277,978 bp
  • A to G, chromosome 7 at 107,814,602 bp
  • T to A, chromosome 8 at 33,671,528 bp
  • G to A, chromosome 8 at 91,248,722 bp
  • T to A, chromosome 9 at 55,206,023 bp
  • A to T, chromosome 11 at 83,214,938 bp
  • T to C, chromosome 11 at 96,961,349 bp
  • A to G, chromosome 11 at 116,059,110 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • A to G, chromosome 12 at 105,604,274 bp
  • G to T, chromosome 13 at 19,192,716 bp
  • A to G, chromosome 13 at 25,107,698 bp
  • A to G, chromosome 15 at 89,475,339 bp
  • C to T, chromosome 16 at 59,344,903 bp
  • T to A, chromosome 17 at 25,090,700 bp
  • T to C, chromosome 17 at 56,771,467 bp
  • G to A, chromosome 17 at 71,243,970 bp
  • A to T, chromosome 18 at 22,105,591 bp
  • A to G, chromosome 18 at 43,568,108 bp
  • T to C, chromosome 19 at 11,112,461 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5099 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042688-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.