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Strain Name:
C57BL/6J-MtgxR5113Btlr/Mmmh
Stock Number:
042701-MU
Citation ID:
RRID:MMRRC_042701-MU
Other Names:
R5113 (G1), C57BL/6J-MtgxR5113Btlr
Major Collection:

Strain Information

Sipa1l1
Name: signal-induced proliferation-associated 1 like 1
Synonyms: 4931426N11Rik, Spar
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217692
VEGA: 12
Homologene: 9189
Gfpt2
Name: glutamine fructose-6-phosphate transaminase 2
Synonyms: GFAT2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14584
HGNC: HGNC:4242
Homologene: 68439
Hmgcr
Name: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Synonyms: HMG-CoAR, 3-hydroxy-3-methylglutaryl-CoA reductase, Red
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15357
HGNC: HGNC:5006
Homologene: 30994
Cltc
Name: clathrin heavy chain
Synonyms: CHC
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67300
HGNC: HGNC:2092
Homologene: 3572
Ski
Name: ski sarcoma viral oncogene homolog (avian)
Synonyms: 2610001A11Rik, 2310012I02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20481
Homologene: 31124
Mcm3
Name: minichromosome maintenance complex component 3
Synonyms: p1.m, P1, Mcmd
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17215
HGNC: HGNC:6945
Homologene: 1791
Eefsec
Name: eukaryotic elongation factor, selenocysteine-tRNA-specific
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 65967
Homologene: 11073
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,803,681 bp
  • T to C, chromosome 1 at 58,991,174 bp
  • T to C, chromosome 1 at 133,900,977 bp
  • A to T, chromosome 2 at 25,239,351 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • A to T, chromosome 2 at 76,812,900 bp
  • A to T, chromosome 2 at 110,661,480 bp
  • G to T, chromosome 2 at 119,431,945 bp
  • A to G, chromosome 2 at 136,552,441 bp
  • T to C, chromosome 2 at 170,114,058 bp
  • T to C, chromosome 3 at 89,066,275 bp
  • A to T, chromosome 3 at 92,669,135 bp
  • A to G, chromosome 3 at 104,784,674 bp
  • A to T, chromosome 3 at 106,017,978 bp
  • G to A, chromosome 3 at 152,243,888 bp
  • C to T, chromosome 4 at 47,030,795 bp
  • A to G, chromosome 4 at 55,530,481 bp
  • T to C, chromosome 4 at 155,159,392 bp
  • A to G, chromosome 4 at 156,048,506 bp
  • T to C, chromosome 5 at 30,920,620 bp
  • A to G, chromosome 6 at 58,265,858 bp
  • A to G, chromosome 6 at 68,303,085 bp
  • A to T, chromosome 6 at 78,466,561 bp
  • C to A, chromosome 6 at 88,281,575 bp
  • A to G, chromosome 7 at 25,015,527 bp
  • A to G, chromosome 7 at 126,797,696 bp
  • C to A, chromosome 8 at 45,270,697 bp
  • A to G, chromosome 8 at 71,989,058 bp
  • T to C, chromosome 9 at 14,464,113 bp
  • T to C, chromosome 9 at 39,949,925 bp
  • T to A, chromosome 9 at 72,979,893 bp
  • T to A, chromosome 9 at 106,066,960 bp
  • A to G, chromosome 9 at 108,082,247 bp
  • A to G, chromosome 10 at 78,970,203 bp
  • A to G, chromosome 10 at 129,268,666 bp
  • A to G, chromosome 11 at 49,823,799 bp
  • A to G, chromosome 11 at 70,315,995 bp
  • G to A, chromosome 11 at 82,961,696 bp
  • A to T, chromosome 11 at 86,722,321 bp
  • A to T, chromosome 11 at 114,804,317 bp
  • A to G, chromosome 11 at 121,147,799 bp
  • C to T, chromosome 12 at 57,496,499 bp
  • C to T, chromosome 12 at 82,440,908 bp
  • T to C, chromosome 12 at 108,537,337 bp
  • A to T, chromosome 13 at 14,346,029 bp
  • A to G, chromosome 13 at 60,721,778 bp
  • G to A, chromosome 13 at 96,656,732 bp
  • T to C, chromosome 14 at 27,652,872 bp
  • A to G, chromosome 14 at 34,724,754 bp
  • A to C, chromosome 14 at 50,710,914 bp
  • T to C, chromosome 17 at 25,345,399 bp
  • A to G, chromosome 18 at 38,268,880 bp
  • C to A, chromosome 18 at 39,775,383 bp
  • A to G, chromosome 19 at 3,897,723 bp
  • A to G, chromosome 19 at 39,163,438 bp
  • T to C, chromosome X at 16,716,423 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5113 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042701-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.