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Strain Name:
C57BL/6J-MtgxR5226Btlr/Mmmh
Stock Number:
042799-MU
Citation ID:
RRID:MMRRC_042799-MU
Other Names:
R5226 (G1), C57BL/6J-MtgxR5226Btlr
Major Collection:

Strain Information

Slc1a3
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: MGluT1, Eaat1, B430115D02Rik, GLAST, Gmt1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20512
Homologene: 20882
Epb41l4a
Name: erythrocyte membrane protein band 4.1 like 4a
Synonyms: NBL4, Epb4.1l4a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13824
VEGA: 18
Homologene: 8398
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: eIF4GII, 1500002J22Rik, 4930523M17Rik, G1-419-52, repro8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Atad5
Name: ATPase family, AAA domain containing 5
Synonyms: LOC237877, C130052G03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237877
Homologene: 32611
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: GCN1L, G431004K08Rik, Gcn1l1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Recql4
Name: RecQ protein-like 4
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 79456
VEGA: 15
HGNC: HGNC:9949
Homologene: 3144
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 4,348,033 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • C to A, chromosome 1 at 134,304,286 bp
  • G to A, chromosome 1 at 135,257,239 bp
  • C to A, chromosome 2 at 4,752,978 bp
  • T to C, chromosome 2 at 31,457,073 bp
  • T to C, chromosome 2 at 118,703,255 bp
  • A to G, chromosome 4 at 117,128,093 bp
  • A to T, chromosome 4 at 134,578,369 bp
  • C to A, chromosome 4 at 138,096,794 bp
  • A to C, chromosome 5 at 98,976,462 bp
  • A to T, chromosome 5 at 114,021,033 bp
  • T to C, chromosome 5 at 115,588,067 bp
  • A to G, chromosome 5 at 123,794,172 bp
  • T to C, chromosome 5 at 136,370,173 bp
  • C to T, chromosome 5 at 140,714,846 bp
  • T to C, chromosome 5 at 145,365,697 bp
  • T to A, chromosome 6 at 37,536,014 bp
  • C to T, chromosome 6 at 83,068,930 bp
  • C to T, chromosome 6 at 101,153,311 bp
  • T to A, chromosome 7 at 16,908,720 bp
  • C to T, chromosome 7 at 20,844,244 bp
  • A to G, chromosome 7 at 79,722,699 bp
  • C to A, chromosome 7 at 143,685,785 bp
  • T to A, chromosome 8 at 41,196,999 bp
  • A to T, chromosome 8 at 117,577,874 bp
  • T to C, chromosome 9 at 3,000,937 bp
  • A to G, chromosome 9 at 67,945,553 bp
  • T to A, chromosome 9 at 69,364,141 bp
  • T to C, chromosome 9 at 124,294,076 bp
  • A to G, chromosome 10 at 79,194,040 bp
  • A to T, chromosome 11 at 50,942,589 bp
  • T to C, chromosome 11 at 80,095,062 bp
  • A to T, chromosome 11 at 83,187,549 bp
  • A to G, chromosome 11 at 99,004,205 bp
  • T to C, chromosome 11 at 119,081,928 bp
  • C to T, chromosome 12 at 8,713,458 bp
  • T to C, chromosome 12 at 112,852,148 bp
  • A to G, chromosome 13 at 38,186,770 bp
  • G to A, chromosome 13 at 38,205,117 bp
  • C to T, chromosome 13 at 60,896,332 bp
  • A to G, chromosome 14 at 37,072,353 bp
  • A to G, chromosome 14 at 120,022,253 bp
  • C to T, chromosome 15 at 8,642,225 bp
  • T to C, chromosome 15 at 76,710,129 bp
  • T to A, chromosome 15 at 97,703,223 bp
  • G to T, chromosome 15 at 98,776,614 bp
  • A to G, chromosome 16 at 13,625,552 bp
  • G to T, chromosome 16 at 29,248,279 bp
  • T to C, chromosome 16 at 56,007,148 bp
  • A to G, chromosome 17 at 21,588,243 bp
  • T to A, chromosome 17 at 24,534,133 bp
  • A to T, chromosome 17 at 34,533,287 bp
  • A to T, chromosome 17 at 46,983,270 bp
  • T to A, chromosome 17 at 68,764,722 bp
  • C to T, chromosome 18 at 33,810,313 bp
  • T to C, chromosome 18 at 37,752,524 bp
  • C to A, chromosome 18 at 57,879,020 bp
  • A to G, chromosome 19 at 6,346,504 bp
  • A to G, chromosome 19 at 10,867,144 bp
  • A to C, chromosome 19 at 46,898,629 bp
  • G to T, chromosome 19 at 57,650,335 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5226 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042799-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.