Strain Name:
C57BL/6J-MtgxR5254Btlr/Mmmh
Stock Number:
042825-MU
Citation ID:
RRID:MMRRC_042825-MU
Other Names:
R5254 (G1), C57BL/6J-MtgxR5254Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, Myo5, flail, 9630007J19Rik, MyoVA, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: HALR, E330008K23Rik, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Vcan
Name: versican
Synonyms: hdf, DPEAAE, heart defect, 5430420N07Rik, PG-M, Cspg2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Nfia
Name: nuclear factor I/A
Synonyms: 9430022M17Rik, NF1A, NF1-A, 1110047K16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18027
HGNC: HGNC:7784
Homologene: 4086
Il21
Name: interleukin 21
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 60505
HGNC: HGNC:6005
Homologene: 11032
Dst
Name: dystonin
Synonyms: Macf2, A830042E19Rik, Bpag1, Bpag, bullous pemphigoid antigen 1, nmf203, athetoid, nmf339, BPAG1, BPAG1-n, 2310001O04Rik, bullous pemphigoid antigen 1, ah
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Bclaf1
Name: BCL2-associated transcription factor 1
Synonyms: 2810454G14Rik, 2610102K23Rik, 5730534O06Rik, 2700025J07Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72567
Homologene: 8832
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: IGF-1R, A330103N21Rik, hyft, CD221, line 186
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Hira
Name: histone cell cycle regulator
Synonyms: Gm15797, Tuple1, D16Ertd95e
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 15260
HGNC: HGNC:4916
Homologene: 48172
Daam1
Name: dishevelled associated activator of morphogenesis 1
Synonyms: 2310028E21Rik, 1700066F09Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 208846
VEGA: 12
Homologene: 36635
Ect2
Name: ect2 oncogene
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 13605
HGNC: HGNC:3155
Homologene: 7298
Mdc1
Name: mediator of DNA damage checkpoint 1
Synonyms: NFBD1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240087
Homologene: 67092
Chaf1a
Name: chromatin assembly factor 1, subunit A
Synonyms: CAF-1, p150
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 27221
VEGA: 17
HGNC: HGNC:1910
Homologene: 4003
Cald1
Name: caldesmon 1
Synonyms: C920027I18Rik, 4833423D12Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109624
HGNC: HGNC:1441
Homologene: 137254
Adam11
Name: a disintegrin and metallopeptidase domain 11
Synonyms: Mdc
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11488
HGNC: HGNC:189
Homologene: 7614
Eya3
Name: EYA transcriptional coactivator and phosphatase 3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14050
HGNC: HGNC:3521
Homologene: 1508
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Mbip
Name: MAP3K12 binding inhibitory protein 1
Synonyms: 4933408E06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217588
VEGA: 12
Homologene: 9576
Trp53inp1
Name: transformation related protein 53 inducible nuclear protein 1
Synonyms: Teap, Stinp, 2700057G22Rik, SIP18, SIP27, Tp53inp1, SIP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 60599
Homologene: 11065
Flt3
Name: FMS-like tyrosine kinase 3
Synonyms: wmfl, Flk-2, Flk2, Flt-3, CD135
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14255
HGNC: HGNC:3765
Homologene: 3040
Tnip2
Name: TNFAIP3 interacting protein 2
Synonyms: ABIN-2, 1810020H16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231130
Homologene: 11515
Atp4a
Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide
Synonyms: H+K+-transporting alpha 1, H+/K+-ATPase alpha
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11944
HGNC: HGNC:819
Homologene: 68081
Pde4a
Name: phosphodiesterase 4A, cAMP specific
Synonyms: Dpde2, dunce, D9Ertd60e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18577
HGNC: HGNC:8780
Homologene: 4520
Abhd8
Name: abhydrolase domain containing 8
Synonyms: 0910001L24Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 64296
Homologene: 11188
Cfap46
Name: cilia and flagella associated protein 46
Synonyms: Ttc40, 9330101J02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 212124
H2-K1
Name: histocompatibility 2, K1, K region
Synonyms: H-2K
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14972
Homologene: 128352
Tarbp1
Name: TAR RNA binding protein 1
Synonyms: Gm17296
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 212728
Homologene: 38082
Fat2
Name: FAT atypical cadherin 2
Synonyms: EMI2, mKIAA0811, LOC245827, Fath2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Exph5
Name: exophilin 5
Synonyms: AC079869.22gm5, slac2-b, B130009M24Rik, Slac2b
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320051
Homologene: 9007
Polq
Name: polymerase (DNA directed), theta
Synonyms: A430110D14Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 77782
HGNC: HGNC:9186
Homologene: 32727
Gbgt1
Name: globoside alpha-1,3-N-acetylgalactosaminyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227671
Homologene: 110677
Cdsn
Name: corneodesmosin
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 386463
HGNC: HGNC:1802
Or14a259
Name: olfactory receptor family 14 subfamily A member 259
Synonyms: MOR219-2, Olfr305, GA_x6K02T2NHDJ-9744055-9745014
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258609
Homologene: 128128
Muc5b
Name: mucin 5, subtype B, tracheobronchial
Synonyms: MUC5, 2300002I04Rik, MUC9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74180
HGNC: HGNC:7516
Homologene: 136756
Dcaf10
Name: DDB1 and CUL4 associated factor 10
Synonyms: Wdr32
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242418
Homologene: 32581
Umodl1
Name: uromodulin-like 1
Synonyms: D17Ertd488e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 52020
VEGA: 17
Homologene: 45466
Ankhd1
Name: ankyrin repeat and KH domain containing 1
Synonyms: 9130019P20Rik, 4933432B13Rik, A530027J04Rik, 1110004O12Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 108857
Homologene: 87006
Vmn2r25
Name: vomeronasal 2, receptor 25
Synonyms: EG545874
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 545874
Homologene: 135915
Slc5a4a
Name: solute carrier family 5, member 4a
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 64452
VEGA: 10
Myo5b
Name: myosin VB
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17919
HGNC: HGNC:7603
Homologene: 49481
Slc22a30
Name: solute carrier family 22, member 30
Synonyms: C730048C13Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 319800
Homologene: 77136
Fam20b
Name: FAM20B, glycosaminoglycan xylosylkinase
Synonyms: C530043G21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 215015
Homologene: 8909
Tmprss11f
Name: transmembrane protease, serine 11f
Synonyms: 4732406D01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243083
Homologene: 65356
Tsfm
Name: Ts translation elongation factor, mitochondrial
Synonyms: EF-TS, EF-Tsmt, 2310050B20Rik, 9430024O13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66399
Homologene: 4184
Wrap73
Name: WD repeat containing, antisense to Trp73
Synonyms: Wdr8, DD57, 5330425N03Rik, 2610044M17Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 59002
Homologene: 9857
Ttc28
Name: tetratricopeptide repeat domain 28
Synonyms: 2310015L07Rik, TPRBK
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209683
Homologene: 41023
Krtap16-1
Name: keratin associated protein 16-1
Synonyms: AI450886
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100504183
Homologene: 99988
Mog
Name: myelin oligodendrocyte glycoprotein
Synonyms: B230317G11Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17441
HGNC: HGNC:7197
Homologene: 111009
Arrdc5
Name: arrestin domain containing 5
Synonyms: 1700013E09Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76920
VEGA: 17
Homologene: 66277
Galnt15
Name: polypeptide N-acetylgalactosaminyltransferase 15
Synonyms: 4631401E18Rik, Galntl2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 78754
Homologene: 14286
Nkd1
Name: naked cuticle 1
Synonyms: 9030215G15Rik, 2810434J10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93960
Homologene: 12343
Or4k15b
Name: olfactory receptor family 4 subfamily K member 15B
Synonyms: MOR246-3, Olfr725, MOR246-7_p, GA_x6K02T2PMLR-5725741-5724776
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 258314
Homologene: 44957
Vmn2r124
Name: vomeronasal 2, receptor 124
Synonyms: Vmn2r-ps113, Gm7196
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 637021
Homologene: 115024
Pcdhb17
Name: protocadherin beta 17
Synonyms: PcdhbQ, Pcdhb16
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93888
Homologene: 81881
Or5an1c
Name: olfactory receptor family 5 subfamily AN member 1C
Synonyms: MOR214-9, Olfr262, GA_x6K02T2N4A9-18144-19082, MOR214-1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258683
Map2k1
Name: mitogen-activated protein kinase kinase 1
Synonyms: Prkmk1, Mek1, MAP kinase kinase 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 26395
HGNC: HGNC:6840
Homologene: 2063
Ggt1
Name: gamma-glutamyltransferase 1
Synonyms: Ggtp, CD224, dwg, GGT
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14598
Homologene: 68450
Or5j3
Name: olfactory receptor family 5 subfamily J member 3
Synonyms: MOR172-1, GA_x6K02T2Q125-47777498-47778436, Olfr1052
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259012
Homologene: 79679
Or8k24
Name: olfactory receptor family 8 subfamily K member 24
Synonyms: Olfr1058, MOR190-2, GA_x6K02T2Q125-47855818-47854868
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258386
Homologene: 74093
Krt1
Name: keratin 1
Synonyms: Krt-2.1, Krt2-1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16678
VEGA: 15
HGNC: HGNC:6412
Homologene: 38146
Or8g55
Name: olfactory receptor family 8 subfamily G member 55
Synonyms: MOR171-17, Olfr972, GA_x6K02T2PVTD-33572803-33573747
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258603
VEGA: 9
Homologene: 121532
Asic5
Name: acid-sensing ion channel family member 5
Synonyms: Accn5, BLINaC, brain-liver-intestine amiloride-sensitive sodium channel
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 58170
Homologene: 41171
Tas2r134
Name: taste receptor, type 2, member 134
Synonyms: T2R134, Tas2r34
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 387511
Homologene: 66001
Cd200r4
Name: CD200 receptor 4
Synonyms: F630107N04Rik, MCD200RLa
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239849
Homologene: 45557
Tmprss11a
Name: transmembrane protease, serine 11a
Synonyms: LOC194597
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 194597
Homologene: 62723
Gm2058
Name: predicted gene 2058
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100039133
Or52z14
Name: olfactory receptor family 52 subfamily Z member 14
Synonyms: Olfr619, GA_x6K02T2PBJ9-6326488-6327450, MOR31-5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259080
Homologene: 45095
Chil4
Name: chitinase-like 4
Synonyms: Chi3l4, Ym2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 104183
Homologene: 74931
Epm2a
Name: epilepsy, progressive myoclonic epilepsy, type 2 gene alpha
Synonyms: laforin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13853
HGNC: HGNC:3413
Homologene: 38087
Tgfb2
Name: transforming growth factor, beta 2
Synonyms: Tgf-beta2, Tgfb-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21808
Homologene: 2432
Fndc11
Name: fibronectin type III domain containing 11
Synonyms: BC051628, 1700026M20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 332713
Homologene: 11442
Mrgprx3-ps
Name: MAS-related GPR, member X3, pseudogene
Synonyms: Gm19419, LOC269919, Gm660
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100502859
Pcdhga8
Name: protocadherin gamma subfamily A, 8
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93716
HGNC: HGNC:8706
Homologene: 57162
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 34,177,931 bp
  • C to T, chromosome 1 at 156,705,740 bp
  • A to G, chromosome 1 at 186,704,483 bp
  • A to G, chromosome 2 at 28,505,208 bp
  • T to G, chromosome 2 at 51,627,547 bp
  • A to T, chromosome 2 at 86,297,921 bp
  • A to T, chromosome 2 at 86,386,140 bp
  • A to G, chromosome 2 at 181,222,163 bp
  • T to C, chromosome 3 at 27,130,070 bp
  • T to C, chromosome 3 at 37,227,735 bp
  • T to A, chromosome 3 at 82,020,987 bp
  • T to A, chromosome 3 at 106,219,452 bp
  • T to A, chromosome 4 at 11,165,075 bp
  • T to A, chromosome 4 at 45,370,415 bp
  • T to A, chromosome 4 at 98,014,297 bp
  • T to A, chromosome 4 at 132,694,754 bp
  • A to G, chromosome 4 at 154,155,346 bp
  • G to A, chromosome 5 at 25,314,594 bp
  • G to T, chromosome 5 at 34,503,578 bp
  • C to A, chromosome 5 at 86,411,806 bp
  • T to A, chromosome 5 at 86,538,033 bp
  • C to T, chromosome 5 at 111,271,238 bp
  • T to A, chromosome 5 at 147,375,690 bp
  • A to G, chromosome 6 at 34,746,416 bp
  • C to G, chromosome 6 at 123,825,318 bp
  • A to T, chromosome 7 at 30,715,530 bp
  • A to T, chromosome 7 at 39,589,234 bp
  • T to A, chromosome 7 at 47,309,436 bp
  • T to A, chromosome 7 at 68,207,319 bp
  • A to T, chromosome 7 at 86,364,190 bp
  • T to G, chromosome 7 at 103,603,789 bp
  • T to A, chromosome 7 at 139,678,514 bp
  • C to T, chromosome 7 at 141,864,540 bp
  • G to T, chromosome 8 at 71,458,398 bp
  • A to G, chromosome 8 at 88,589,194 bp
  • G to A, chromosome 8 at 126,467,156 bp
  • G to T, chromosome 9 at 21,201,258 bp
  • A to T, chromosome 9 at 39,873,445 bp
  • A to T, chromosome 9 at 53,337,930 bp
  • A to T, chromosome 9 at 64,187,745 bp
  • A to T, chromosome 9 at 75,130,120 bp
  • C to A, chromosome 10 at 11,457,345 bp
  • T to A, chromosome 10 at 20,323,536 bp
  • T to A, chromosome 10 at 75,579,198 bp
  • C to A, chromosome 10 at 76,182,738 bp
  • C to A, chromosome 10 at 127,011,761 bp
  • T to C, chromosome 11 at 55,281,175 bp
  • G to A, chromosome 11 at 99,985,598 bp
  • T to A, chromosome 11 at 102,774,272 bp
  • A to G, chromosome 12 at 56,337,443 bp
  • A to T, chromosome 12 at 71,946,576 bp
  • A to T, chromosome 13 at 13,683,070 bp
  • A to T, chromosome 13 at 89,691,600 bp
  • C to T, chromosome 14 at 32,058,287 bp
  • C to A, chromosome 14 at 50,034,678 bp
  • A to T, chromosome 15 at 101,846,368 bp
  • T to C, chromosome 16 at 18,916,583 bp
  • A to T, chromosome 16 at 37,089,319 bp
  • A to C, chromosome 16 at 44,832,090 bp
  • T to A, chromosome 17 at 18,063,077 bp
  • T to G, chromosome 17 at 30,980,359 bp
  • T to C, chromosome 17 at 33,997,462 bp
  • T to A, chromosome 17 at 35,552,202 bp
  • T to A, chromosome 17 at 35,847,922 bp
  • T to G, chromosome 17 at 37,012,372 bp
  • T to C, chromosome 17 at 56,062,606 bp
  • A to G, chromosome 17 at 56,297,897 bp
  • T to A, chromosome 17 at 67,756,716 bp
  • A to G, chromosome 18 at 36,656,715 bp
  • A to T, chromosome 18 at 37,486,825 bp
  • A to T, chromosome 18 at 37,726,620 bp
  • A to T, chromosome 18 at 74,700,606 bp
  • G to A, chromosome 19 at 8,344,393 bp
  • A to G, chromosome 19 at 12,241,248 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5254 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042825-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.