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Strain Name:
C57BL/6J-MtgxR5258Btlr/Mmmh
Stock Number:
042828-MU
Citation ID:
RRID:MMRRC_042828-MU
Other Names:
R5258 (G1), C57BL/6J-MtgxR5258Btlr
Major Collection:

Strain Information

Card11
Name: caspase recruitment domain family, member 11
Synonyms: CARMA1, BIMP3, 2410011D02Rik, 0610008L17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108723
Homologene: 13024
Prdm16
Name: PR domain containing 16
Synonyms: Mel1, 5730557K01Rik, line 27, csp1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 70673
Homologene: 11139
P2rx7
Name: purinergic receptor P2X, ligand-gated ion channel, 7
Synonyms: P2X7 receptor, P2X7R, P2X(7)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18439
HGNC: HGNC:8537
Homologene: 1925
Mrps6
Name: mitochondrial ribosomal protein S6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 121022
Homologene: 41871
Abcc8
Name: ATP-binding cassette, sub-family C member 8
Synonyms: SUR1, Sur, D930031B21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20927
HGNC: HGNC:59
Homologene: 68048
Mib1
Name: MIB E3 ubiquitin protein ligase 1
Synonyms: E430019M12Rik, Mib, skeletrophin, mind bomb-1, mindbomb
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225164
Homologene: 10810
Tfdp1
Name: transcription factor Dp 1
Synonyms: Dp1, Drtf1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 21781
Homologene: 38276
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 86,305,565 bp
  • G to A, chromosome 1 at 97,840,754 bp
  • A to T, chromosome 2 at 88,093,418 bp
  • T to A, chromosome 2 at 120,699,343 bp
  • C to G, chromosome 3 at 86,987,647 bp
  • A to G, chromosome 4 at 48,396,374 bp
  • T to C, chromosome 4 at 121,615,700 bp
  • T to C, chromosome 4 at 133,247,642 bp
  • A to T, chromosome 4 at 154,367,214 bp
  • T to C, chromosome 5 at 5,604,271 bp
  • A to G, chromosome 5 at 122,681,003 bp
  • G to A, chromosome 5 at 140,876,425 bp
  • A to T, chromosome 6 at 37,919,400 bp
  • T to C, chromosome 6 at 48,476,494 bp
  • T to A, chromosome 6 at 69,016,827 bp
  • T to A, chromosome 6 at 131,689,541 bp
  • T to A, chromosome 7 at 6,066,623 bp
  • T to A, chromosome 7 at 25,348,326 bp
  • T to C, chromosome 7 at 46,108,387 bp
  • C to T, chromosome 7 at 46,157,148 bp
  • C to T, chromosome 7 at 105,674,037 bp
  • T to G, chromosome 7 at 120,436,769 bp
  • C to T, chromosome 7 at 135,410,527 bp
  • G to A, chromosome 8 at 3,512,718 bp
  • A to T, chromosome 8 at 4,628,456 bp
  • C to T, chromosome 8 at 13,369,529 bp
  • C to A, chromosome 8 at 22,627,015 bp
  • C to T, chromosome 8 at 83,969,267 bp
  • T to C, chromosome 9 at 50,764,333 bp
  • T to C, chromosome 9 at 104,309,475 bp
  • T to C, chromosome 9 at 106,996,925 bp
  • C to A, chromosome 10 at 18,524,322 bp
  • T to A, chromosome 10 at 39,922,021 bp
  • C to T, chromosome 10 at 41,894,988 bp
  • A to T, chromosome 11 at 9,249,684 bp
  • A to C, chromosome 11 at 50,923,090 bp
  • G to C, chromosome 11 at 60,711,563 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • T to C, chromosome 11 at 82,904,946 bp
  • T to A, chromosome 11 at 103,635,073 bp
  • T to A, chromosome 11 at 106,567,759 bp
  • A to G, chromosome 12 at 4,236,481 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • T to C, chromosome 13 at 55,653,024 bp
  • A to G, chromosome 14 at 12,142,431 bp
  • T to A, chromosome 14 at 32,663,309 bp
  • C to T, chromosome 14 at 53,554,273 bp
  • A to G, chromosome 15 at 35,794,421 bp
  • A to T, chromosome 15 at 74,716,391 bp
  • T to A, chromosome 16 at 92,099,655 bp
  • A to G, chromosome 17 at 17,712,852 bp
  • G to A, chromosome 18 at 10,795,856 bp
  • C to T, chromosome 18 at 84,315,926 bp
  • A to G, chromosome 19 at 8,884,089 bp
  • T to A, chromosome 19 at 33,613,843 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5258 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042828-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.