Strain Name:
C57BL/6J-MtgxR5258Btlr/Mmmh
Stock Number:
042828-MU
Citation ID:
RRID:MMRRC_042828-MU
Other Names:
R5258 (G1), C57BL/6J-MtgxR5258Btlr
Major Collection:

Gene Information

Card11
Name: caspase recruitment domain family, member 11
Synonyms: BIMP3, 2410011D02Rik, CARMA1, 0610008L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108723
Homologene: 13024
Prdm16
Name: PR domain containing 16
Synonyms: 5730557K01Rik, csp1, Mel1, line 27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70673
Homologene: 11139
P2rx7
Name: purinergic receptor P2X, ligand-gated ion channel, 7
Synonyms: P2X7 receptor, P2X7R, P2X(7)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18439
HGNC: HGNC:8537
Homologene: 1925
Mrps6
Name: mitochondrial ribosomal protein S6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 121022
Homologene: 41871
Abcc8
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 8
Synonyms: D930031B21Rik, Sur, SUR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20927
HGNC: HGNC:59
Homologene: 68048
Mib1
Name: mindbomb E3 ubiquitin protein ligase 1
Synonyms: mind bomb-1, Mib, skeletrophin, E430019M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225164
Homologene: 10810
Tfdp1
Name: transcription factor Dp 1
Synonyms: Dp1, Drtf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 21781
Homologene: 38276
Ptprg
Name: protein tyrosine phosphatase, receptor type, G
Synonyms: 5430405N12Rik, RPTPgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19270
HGNC: HGNC:9671
Homologene: 2129
Asf1b
Name: anti-silencing function 1B histone chaperone
Synonyms: 1700003K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66929
Homologene: 56797
Tex2
Name: testis expressed gene 2
Synonyms: 4930568E07Rik, Taz4, Def-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21763
Homologene: 32414
Trim24
Name: tripartite motif-containing 24
Synonyms: A130082H20Rik, TIF1alpha, D430004I05Rik, Tif1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21848
Homologene: 20830
Ddx46
Name: DEAD box helicase 46
Synonyms: 2200005K02Rik, 8430438J23Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 212880
VEGA: 13
Homologene: 5430
Zfp407
Name: zinc finger protein 407
Synonyms: 6430585N13Rik, LOC240469, LOC381139
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240476
Homologene: 86402
Foxi2
Name: forkhead box I2
Synonyms: B130055A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 270004
Homologene: 18838
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 2310042E16Rik, C330002D13Rik, 1810042B05Rik, Coh1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Zfp958
Name: zinc finger protein 958
Synonyms: BC003267
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 233987
Homologene: 129582
Nle1
Name: notchless homolog 1
Synonyms: l11Jus4, notchless, Nle, l11Jus1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217011
Homologene: 5494
Abca16
Name: ATP-binding cassette, sub-family A (ABC1), member 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233810
Homologene: 132942
Mcoln1
Name: mucolipin 1
Synonyms: TRPML1, 2210015I05Rik, mucolipidin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94178
Homologene: 10744
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Invs
Name: inversin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16348
Homologene: 7786
Psca
Name: prostate stem cell antigen
Synonyms: 2210408B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 72373
HGNC: HGNC:9500
Homologene: 4150
Abca13
Name: ATP-binding cassette, sub-family A (ABC1), member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268379
Homologene: 27991
Lyzl6
Name: lysozyme-like 6
Synonyms: 1700023H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69444
Homologene: 10709
Dock3
Name: dedicator of cyto-kinesis 3
Synonyms: Moca, PBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208869
HGNC: HGNC:2989
Homologene: 21030
Stard9
Name: START domain containing 9
Synonyms: E230025N21Rik, N-3 kinesin, Kif16a, 4831403C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668880
Homologene: 130712
Nhsl1
Name: NHS-like 1
Synonyms: 5730409E15Rik, D10Bwg0940e, A630035H13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215819
Homologene: 27834
Cfap69
Name: cilia and flagella associated protein 69
Synonyms: A330021E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 207686
Homologene: 11718
Gm12887
Name: predicted gene 12887
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 666927
Homologene: 111034
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 214321
Homologene: 86950
Megf8
Name: multiple EGF-like-domains 8
Synonyms: Egfl4, b2b1702Clo, b2b288Clo, m687Ddg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Acpp
Name: acid phosphatase, prostate
Synonyms: PAP, A030005E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56318
HGNC: HGNC:125
Homologene: 55552
Pam
Name: peptidylglycine alpha-amidating monooxygenase
Synonyms: PHM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18484
HGNC: HGNC:8596
Homologene: 37369
Sspo
Name: SCO-spondin
Synonyms: Scospondin, C79529
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243369
Homologene: 45453
Map3k6
Name: mitogen-activated protein kinase kinase kinase 6
Synonyms: MAPKKK6, MEKK6, Ask2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53608
HGNC: HGNC:6858
Homologene: 3435
Gm8978
Name: predicted gene 8978
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 668106
Homologene: 86428
Vmn2r90
Name: vomeronasal 2, receptor 90
Synonyms: EG626942
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 626942
3425401B19Rik
Name: RIKEN cDNA 3425401B19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100504518
VEGA: 14
Homologene: 54908
Olfr1164
Name: olfactory receptor 1164
Synonyms: MOR174-11, GA_x6K02T2Q125-49585842-49584862
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258634
Homologene: 74077
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Nlrp4c
Name: NLR family, pyrin domain containing 4C
Synonyms: Rnh2, Nalp-alpha, Nalp4c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 83564
Homologene: 75315
Ptrhd1
Name: peptidyl-tRNA hydrolase domain containing 1
Synonyms: 2410017P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 69709
VEGA: 12
Homologene: 13963
B3gnt7
Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
Synonyms: beta-3GnT7, C330001H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227327
Homologene: 17049
Dkk4
Name: dickkopf WNT signaling pathway inhibitor 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234130
HGNC: HGNC:2894
Homologene: 8680
Cd1d2
Name: CD1d2 antigen
Synonyms: CD1.2, Cd1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12480
HGNC: HGNC:1637
Olfr1
Name: olfactory receptor 1
Synonyms: MOR135-13, GA_x6K02T2P1NL-3556334-3555390, I54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258923
Homologene: 115482
Sesn1
Name: sestrin 1
Synonyms: PA26, SEST1, 1110002G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 140742
Homologene: 8697
Zfp354b
Name: zinc finger protein 354B
Synonyms: Kid2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27274
Homologene: 32187
1110032A03Rik
Name: RIKEN cDNA 1110032A03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68721
HGNC: HGNC:1163
Homologene: 11242
Mfsd4b2
Name: major facilitator superfamily domain containing 4B2
Synonyms: 2010001E11Rik, Mfsd4b2, Mfsd4b2-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 72045
Homologene: 129931
Tas2r114
Name: taste receptor, type 2, member 114
Synonyms: mGR14, mt2r46, Tas2r14, T2R14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387346
Homologene: 128355
Igkv4-80
Name: immunoglobulin kappa variable 4-80
Synonyms: Gm16729
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 545848
Lbhd1
Name: LBH domain containing 1
Synonyms: Gm21743
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 102308570
Homologene: 75203
Trav14-1
Name: T cell receptor alpha variable 14-1
Synonyms: Gm5770
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 436467
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 86,305,565 bp
  • G to A, chromosome 1 at 97,840,754 bp
  • A to T, chromosome 2 at 88,093,418 bp
  • T to A, chromosome 2 at 120,699,343 bp
  • C to G, chromosome 3 at 86,987,647 bp
  • A to G, chromosome 4 at 48,396,374 bp
  • T to C, chromosome 4 at 121,615,700 bp
  • T to C, chromosome 4 at 133,247,642 bp
  • A to T, chromosome 4 at 154,367,214 bp
  • T to C, chromosome 5 at 5,604,271 bp
  • A to G, chromosome 5 at 122,681,003 bp
  • G to A, chromosome 5 at 140,876,425 bp
  • A to T, chromosome 6 at 37,919,400 bp
  • T to C, chromosome 6 at 48,476,494 bp
  • T to A, chromosome 6 at 69,016,827 bp
  • T to A, chromosome 6 at 131,689,541 bp
  • T to A, chromosome 7 at 6,066,623 bp
  • T to A, chromosome 7 at 25,348,326 bp
  • T to C, chromosome 7 at 46,108,387 bp
  • C to T, chromosome 7 at 46,157,148 bp
  • C to T, chromosome 7 at 105,674,037 bp
  • T to G, chromosome 7 at 120,436,769 bp
  • C to T, chromosome 7 at 135,410,527 bp
  • G to A, chromosome 8 at 3,512,718 bp
  • A to T, chromosome 8 at 4,628,456 bp
  • C to T, chromosome 8 at 13,369,529 bp
  • C to A, chromosome 8 at 22,627,015 bp
  • C to T, chromosome 8 at 83,969,267 bp
  • T to C, chromosome 9 at 50,764,333 bp
  • T to C, chromosome 9 at 104,309,475 bp
  • T to C, chromosome 9 at 106,996,925 bp
  • C to A, chromosome 10 at 18,524,322 bp
  • T to A, chromosome 10 at 39,922,021 bp
  • C to T, chromosome 10 at 41,894,988 bp
  • A to T, chromosome 11 at 9,249,684 bp
  • A to C, chromosome 11 at 50,923,090 bp
  • G to C, chromosome 11 at 60,711,563 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • T to C, chromosome 11 at 82,904,946 bp
  • T to A, chromosome 11 at 103,635,073 bp
  • T to A, chromosome 11 at 106,567,759 bp
  • A to G, chromosome 12 at 4,236,481 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • T to C, chromosome 13 at 55,653,024 bp
  • A to G, chromosome 14 at 12,142,431 bp
  • T to A, chromosome 14 at 32,663,309 bp
  • C to T, chromosome 14 at 53,554,273 bp
  • A to G, chromosome 15 at 35,794,421 bp
  • A to T, chromosome 15 at 74,716,391 bp
  • T to A, chromosome 16 at 92,099,655 bp
  • A to G, chromosome 17 at 17,712,852 bp
  • G to A, chromosome 18 at 10,795,856 bp
  • C to T, chromosome 18 at 84,315,926 bp
  • A to G, chromosome 19 at 8,884,089 bp
  • T to A, chromosome 19 at 33,613,843 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5258 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042828-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.