Strain Name:
C57BL/6J-MtgxR5299Btlr/Mmmh
Stock Number:
042882-MU
Citation ID:
RRID:MMRRC_042882-MU
Other Names:
R5299 (G1), C57BL/6J-MtgxR5299Btlr
Major Collection:

Gene Information

Map2k6
Name: mitogen-activated protein kinase kinase 6
Synonyms: SAPKK3, MAP kinase kinase 6, Prkmk6, MKK6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 26399
HGNC: HGNC:6846
Homologene: 55686
Axin1
Name: axin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12005
HGNC: HGNC:903
Homologene: 2614
Arid1a
Name: AT rich interactive domain 1A (SWI-like)
Synonyms: Osa1, 1110030E03Rik, BAF250a, Smarcf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 93760
Homologene: 21216
Ube2g2
Name: ubiquitin-conjugating enzyme E2G 2
Synonyms: Ubc7p, D10Xrf369, 1110003O05Rik, UBC7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22213
Homologene: 6599
Exoc2
Name: exocyst complex component 2
Synonyms: 2410030I24Rik, Sec5, Sec5l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66482
Homologene: 10122
Bend3
Name: BEN domain containing 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 331623
VEGA: 10
Homologene: 19511
Mical3
Name: microtubule associated monooxygenase, calponin and LIM domain containing 3
Synonyms: C130040D16Rik, MICAL-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 194401
Homologene: 85288
Mcph1
Name: microcephaly, primary autosomal recessive 1
Synonyms: BRIT1, 5430437K10Rik, D030046N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244329
HGNC: HGNC:6954
Homologene: 32586
Wls
Name: wntless WNT ligand secretion mediator
Synonyms: 5031439A09Rik, Gpr177
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68151
Homologene: 11779
Wapl
Name: WAPL cohesin release factor
Synonyms: Wapal, A530089A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218914
Homologene: 41002
Slc12a3
Name: solute carrier family 12, member 3
Synonyms: TSC, NCC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20497
Homologene: 287
Ehmt2
Name: euchromatic histone lysine N-methyltransferase 2
Synonyms: KMT1C, G9a, D17Ertd710e, NG36, Bat8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 110147
Homologene: 48460
Ppp1r7
Name: protein phosphatase 1, regulatory subunit 7
Synonyms: 2310014J01Rik, SDS22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66385
HGNC: HGNC:9295
Homologene: 2032
Chodl
Name: chondrolectin
Synonyms: 3110074E07Rik, PRED12, MT75
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 246048
Homologene: 11795
Lgr6
Name: leucine-rich repeat-containing G protein-coupled receptor 6
Synonyms: A530037C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329252
Homologene: 49680
Ift81
Name: intraflagellar transport 81
Synonyms: CDV-1R, Cdv1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12589
Homologene: 7664
Spint2
Name: serine protease inhibitor, Kunitz type 2
Synonyms: HAI-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20733
Homologene: 7955
Serinc1
Name: serine incorporator 1
Synonyms: 1500011D18Rik, Tde1l, Tde2, TMS-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56442
Homologene: 41334
Nelfb
Name: negative elongation factor complex member B
Synonyms: Cobra1, A730008L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 58202
Homologene: 121600
Nbas
Name: neuroblastoma amplified sequence
Synonyms: 4933425L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 71169
VEGA: 12
Homologene: 41073
Abca13
Name: ATP-binding cassette, sub-family A (ABC1), member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268379
Homologene: 27991
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, Dnahc2, Dnhd3, 2900022L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Grk2
Name: G protein-coupled receptor kinase 2
Synonyms: beta-AR kinase-1, beta-adrenergic receptor kinase-1, beta ARK1, betaARK1, Bark-1, Adrbk1, beta ARK, Adrbk-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 110355
HGNC: HGNC:289
Homologene: 1223
Otog
Name: otogelin
Synonyms: Otgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18419
HGNC: HGNC:8516
Homologene: 8421
Ppp2r1b
Name: protein phosphatase 2, regulatory subunit A, beta
Synonyms: 2410091N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 73699
HGNC: HGNC:9303
Homologene: 70244
Ubxn8
Name: UBX domain protein 8
Synonyms: Rep8h, Ubxd6, D0H8S2298E, Rep-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108159
Homologene: 4149
Traf3ip3
Name: TRAF3 interacting protein 3
Synonyms: 6030423D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 215243
Homologene: 11885
Atxn1
Name: ataxin 1
Synonyms: Sca1, 2900016G23Rik, Atx1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20238
VEGA: 13
Homologene: 281
Kcnip1
Name: Kv channel-interacting protein 1
Synonyms: KCHIP1, 2900046L02Rik, 3202002F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70357
Homologene: 22824
Rhof
Name: ras homolog family member F (in filopodia)
Synonyms: Ifld1, Arhf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 23912
Homologene: 41304
Ipo4
Name: importin 4
Synonyms: 8430408O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75751
Homologene: 5835
Rsbn1
Name: rosbin, round spermatid basic protein 1
Synonyms: C230004D03Rik, Rsbp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229675
Homologene: 10154
Igtp
Name: interferon gamma induced GTPase
Synonyms: Irgm3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16145
Homologene: 23118
Zfp503
Name: zinc finger protein 503
Synonyms: Nolz-1, Nolz1, B830002A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218820
Homologene: 41888
Slc25a24
Name: solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 24
Synonyms: 2610016M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229731
Homologene: 92693
Proca1
Name: protein interacting with cyclin A1
Synonyms: 4933404M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216974
Homologene: 51856
Skint4
Name: selection and upkeep of intraepithelial T cells 4
Synonyms: 9530098N22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320640
Vmn1r38
Name: vomeronasal 1 receptor 38
Synonyms: V1rc13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171186
Homologene: 110800
Vmn1r200
Name: vomeronasal 1 receptor 200
Synonyms: V1rh3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 171246
Homologene: 110880
Mdfi
Name: MyoD family inhibitor
Synonyms: I-mf, I-mfa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17240
HGNC: HGNC:6967
Homologene: 21150
Wfdc6a
Name: WAP four-disulfide core domain 6A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 209351
Homologene: 128601
Ighv1-15
Name: immunoglobulin heavy variable 1-15
Synonyms: Gm16858
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 629865
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 93,352,626 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • T to A, chromosome 1 at 193,178,175 bp
  • A to G, chromosome 2 at 25,210,745 bp
  • T to C, chromosome 2 at 164,580,391 bp
  • G to C, chromosome 3 at 103,914,490 bp
  • A to G, chromosome 3 at 109,166,352 bp
  • A to T, chromosome 3 at 159,911,766 bp
  • A to T, chromosome 4 at 112,136,006 bp
  • A to T, chromosome 4 at 133,687,226 bp
  • T to C, chromosome 5 at 122,607,056 bp
  • A to G, chromosome 5 at 123,120,548 bp
  • T to A, chromosome 6 at 66,776,698 bp
  • A to G, chromosome 6 at 120,959,512 bp
  • G to A, chromosome 7 at 29,263,726 bp
  • A to G, chromosome 7 at 46,288,851 bp
  • T to C, chromosome 8 at 18,652,580 bp
  • C to A, chromosome 8 at 33,641,919 bp
  • A to G, chromosome 8 at 94,351,789 bp
  • A to G, chromosome 9 at 50,857,040 bp
  • G to A, chromosome 10 at 43,493,690 bp
  • A to G, chromosome 10 at 57,523,051 bp
  • T to C, chromosome 10 at 77,644,545 bp
  • A to G, chromosome 11 at 9,431,861 bp
  • G to A, chromosome 11 at 33,643,397 bp
  • T to C, chromosome 11 at 58,207,133 bp
  • C to T, chromosome 11 at 69,458,920 bp
  • T to C, chromosome 11 at 78,205,252 bp
  • A to G, chromosome 11 at 110,492,963 bp
  • C to T, chromosome 12 at 13,441,925 bp
  • T to C, chromosome 12 at 114,657,378 bp
  • T to A, chromosome 13 at 22,395,775 bp
  • A to T, chromosome 13 at 30,871,918 bp
  • A to T, chromosome 13 at 45,557,254 bp
  • A to G, chromosome 14 at 21,985,439 bp
  • G to A, chromosome 14 at 34,733,808 bp
  • A to G, chromosome 14 at 55,627,879 bp
  • C to T, chromosome 16 at 78,941,408 bp
  • A to G, chromosome 17 at 26,173,734 bp
  • C to T, chromosome 17 at 34,899,091 bp
  • A to G, chromosome 17 at 47,820,834 bp
  • T to C, chromosome 19 at 4,292,771 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5299 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042882-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.