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Strain Name:
C57BL/6J-MtgxR5395Btlr/Mmmh
Stock Number:
042967-MU
Citation ID:
RRID:MMRRC_042967-MU
Other Names:
R5395 (G1), C57BL/6J-MtgxR5395Btlr
Major Collection:

Strain Information

Tyr
Name: tyrosinase
Synonyms: skc35, Oca1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22173
Homologene: 30969
Itgb8
Name: integrin beta 8
Synonyms: 4832412O06Rik, D630049N15
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320910
HGNC: HGNC:6163
Homologene: 18567
Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Smyd1
Name: SET and MYND domain containing 1
Synonyms: Bop, 4632404M21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12180
Homologene: 7645
Gdnf
Name: glial cell line derived neurotrophic factor
Synonyms: glial cell line-derived neurotrophic factor
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14573
VEGA: 15
HGNC: HGNC:4232
Homologene: 433
Snd1
Name: staphylococcal nuclease and tudor domain containing 1
Synonyms: p100 co-activator, Tudor-SN
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56463
Homologene: 8665
Rangap1
Name: RAN GTPase activating protein 1
Synonyms: Fug1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19387
VEGA: 15
HGNC: HGNC:9854
Homologene: 55700
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,882,664 bp
  • T to C, chromosome 1 at 75,197,939 bp
  • T to A, chromosome 1 at 87,596,675 bp
  • G to T, chromosome 1 at 172,598,720 bp
  • A to T, chromosome 1 at 173,183,680 bp
  • T to A, chromosome 1 at 175,755,598 bp
  • T to A, chromosome 2 at 29,737,965 bp
  • A to G, chromosome 2 at 30,714,940 bp
  • A to T, chromosome 2 at 37,802,128 bp
  • A to T, chromosome 2 at 60,365,111 bp
  • A to T, chromosome 2 at 129,208,337 bp
  • T to A, chromosome 2 at 154,864,007 bp
  • T to A, chromosome 2 at 167,467,722 bp
  • G to A, chromosome 2 at 177,832,984 bp
  • C to A, chromosome 3 at 87,971,766 bp
  • A to G, chromosome 3 at 122,080,941 bp
  • A to T, chromosome 3 at 123,556,756 bp
  • T to C, chromosome 4 at 4,016,277 bp
  • G to A, chromosome 4 at 41,118,392 bp
  • C to T, chromosome 4 at 103,211,375 bp
  • T to C, chromosome 4 at 140,570,290 bp
  • C to A, chromosome 5 at 30,890,338 bp
  • T to C, chromosome 5 at 93,269,398 bp
  • A to G, chromosome 5 at 137,528,526 bp
  • A to G, chromosome 5 at 147,354,823 bp
  • T to A, chromosome 6 at 28,526,184 bp
  • T to G, chromosome 6 at 37,957,744 bp
  • T to G, chromosome 6 at 71,219,390 bp
  • T to C, chromosome 6 at 119,271,418 bp
  • T to C, chromosome 7 at 12,651,947 bp
  • A to G, chromosome 7 at 85,750,897 bp
  • C to A, chromosome 7 at 87,472,490 bp
  • A to T, chromosome 7 at 99,935,160 bp
  • G to A, chromosome 8 at 22,243,691 bp
  • G to A, chromosome 8 at 75,123,026 bp
  • T to A, chromosome 8 at 78,435,072 bp
  • A to G, chromosome 8 at 86,017,468 bp
  • T to A, chromosome 8 at 124,545,221 bp
  • T to C, chromosome 9 at 7,861,174 bp
  • T to C, chromosome 9 at 26,889,632 bp
  • G to A, chromosome 9 at 57,105,673 bp
  • G to A, chromosome 9 at 57,753,339 bp
  • A to G, chromosome 9 at 74,849,180 bp
  • A to T, chromosome 10 at 53,538,692 bp
  • T to A, chromosome 10 at 74,185,287 bp
  • T to C, chromosome 10 at 107,817,138 bp
  • T to C, chromosome 10 at 127,595,297 bp
  • G to A, chromosome 11 at 3,688,232 bp
  • T to C, chromosome 11 at 53,433,797 bp
  • A to T, chromosome 11 at 59,177,489 bp
  • T to A, chromosome 11 at 67,897,358 bp
  • C to T, chromosome 11 at 70,690,438 bp
  • T to C, chromosome 11 at 85,825,249 bp
  • T to G, chromosome 11 at 94,091,751 bp
  • T to A, chromosome 11 at 98,558,213 bp
  • A to T, chromosome 11 at 106,382,473 bp
  • T to C, chromosome 12 at 50,391,432 bp
  • T to A, chromosome 12 at 72,941,701 bp
  • T to A, chromosome 12 at 80,170,703 bp
  • T to G, chromosome 12 at 98,715,117 bp
  • A to C, chromosome 12 at 119,170,741 bp
  • T to A, chromosome 13 at 67,783,846 bp
  • T to C, chromosome 13 at 74,480,546 bp
  • T to A, chromosome 13 at 107,414,493 bp
  • A to G, chromosome 14 at 7,883,881 bp
  • C to A, chromosome 14 at 54,733,277 bp
  • A to C, chromosome 14 at 68,570,903 bp
  • G to T, chromosome 14 at 70,395,397 bp
  • T to A, chromosome 15 at 7,834,684 bp
  • C to T, chromosome 15 at 11,027,785 bp
  • A to G, chromosome 15 at 81,706,446 bp
  • G to A, chromosome 15 at 81,772,501 bp
  • A to T, chromosome 16 at 29,420,888 bp
  • A to G, chromosome 16 at 29,456,604 bp
  • C to T, chromosome 17 at 32,669,853 bp
  • C to A, chromosome 17 at 57,105,592 bp
  • G to A, chromosome 18 at 80,636,020 bp
  • T to C, chromosome 19 at 42,760,572 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5395 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042967-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.