Strain Name:
C57BL/6J-MtgxR5439Btlr/Mmmh
Stock Number:
043004-MU
Citation ID:
RRID:MMRRC_043004-MU
Other Names:
R5439 (G1), C57BL/6J-MtgxR5439Btlr
Major Collection:

Gene Information

Ppp2r2a
Name: protein phosphatase 2, regulatory subunit B, alpha
Synonyms: 2410004D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71978
VEGA: 14
HGNC: HGNC:9304
Homologene: 2035
Arid4b
Name: AT rich interactive domain 4B (RBP1-like)
Synonyms: RBBP1L1, SAP180, BRCAA1, 6720480E17Rik, 6330417L24Rik, Rbp1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 94246
Homologene: 12847
Cep83
Name: centrosomal protein 83
Synonyms: 2600001G24Rik, 5730513H21Rik, 4921537D05Rik, Ccdc41
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77048
VEGA: 10
Homologene: 9396
Timeless
Name: timeless circadian clock 1
Synonyms: tim
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 21853
Homologene: 31206
Ppp2r5e
Name: protein phosphatase 2, regulatory subunit B', epsilon
Synonyms: protein phosphatase 2A subunit beta, 4633401M22Rik, B56beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 26932
VEGA: 12
HGNC: HGNC:9313
Homologene: 55962
Nckap1
Name: NCK-associated protein 1
Synonyms: mh19, Hem-2, H19, Nap1, Hem2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 50884
HGNC: HGNC:7666
Homologene: 8384
Ctbp1
Name: C-terminal binding protein 1
Synonyms: D4S115h, D5H4S115, BARS, D5H4S115E, CtBP1-S, CtBP1-L, CtBP3/BARS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13016
HGNC: HGNC:2494
Homologene: 1015
G3bp1
Name: GTPase activating protein (SH3 domain) binding protein 1
Synonyms: GAP SH3 binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27041
Homologene: 38096
Abca1
Name: ATP-binding cassette, sub-family A (ABC1), member 1
Synonyms: ABC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11303
HGNC: HGNC:29
Homologene: 21130
Actr8
Name: ARP8 actin-related protein 8
Synonyms: ARP8, 5730542K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56249
VEGA: 14
Homologene: 11286
Sqle
Name: squalene epoxidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20775
Homologene: 2355
Jcad
Name: junctional cadherin 5 associated
Synonyms: 9430020K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240185
VEGA: 18
Homologene: 19370
Mcf2l
Name: mcf.2 transforming sequence-like
Synonyms: Dbs, C130040G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17207
Homologene: 11804
Kifc1
Name: kinesin family member C1
Synonyms: Tctex-7, Tctex-7A, Tctex7, Tctex7a, kinesin family c-terminal 5A, HSET, KNSL2, Kifc5a, Gm4137, Knsl2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 100502766
Homologene: 83229
C3
Name: complement component 3
Synonyms: complement factor 3, acylation stimulating protein, Plp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12266
HGNC: HGNC:1318
Homologene: 68031
Il22
Name: interleukin 22
Synonyms: IL-TIF, IL-22, Iltif
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 50929
Homologene: 9669
Pum3
Name: pumilio RNA-binding family member 3
Synonyms: 1110069H02Rik, D19Bwg1357e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 52874
VEGA: 19
Homologene: 5762
Extl3
Name: exostosin-like glycosyltransferase 3
Synonyms: 2900009G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 54616
VEGA: 14
HGNC: HGNC:3518
Homologene: 1103
Pold3
Name: polymerase (DNA-directed), delta 3, accessory subunit
Synonyms: P68, P66, 2410142G14Rik, C85233, GC12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67967
Homologene: 38202
Dnaaf10
Name: dynein axonemal assembly factor 10
Synonyms: Wdr92
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103784
Homologene: 16321
Zfp940
Name: zinc finger protein 940
Synonyms: BC027344
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233057
Homologene: 138421
Papln
Name: papilin, proteoglycan-like sulfated glycoprotein
Synonyms: E030033C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 170721
Homologene: 71541
Plvap
Name: plasmalemma vesicle associated protein
Synonyms: PV-1, MECA32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 84094
Homologene: 10578
Ddr2
Name: discoidin domain receptor family, member 2
Synonyms: Ntrkr3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18214
HGNC: HGNC:2731
Homologene: 68505
Nkx6-1
Name: NK6 homeobox 1
Synonyms: NKX6A, Nkx6.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18096
HGNC: HGNC:7839
Homologene: 4495
Cacna1c
Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
Synonyms: (alpha)1 subunit, Cchl1a1, Cav1.2, L-type Cav1.2, D930026N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12288
HGNC: HGNC:1390
Homologene: 55484
Tex15
Name: testis expressed gene 15
Synonyms: 2210014E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 104271
Homologene: 12837
Med12l
Name: mediator complex subunit 12-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329650
Homologene: 43143
Ralgapa2
Name: Ral GTPase activating protein, alpha subunit 2 (catalytic)
Synonyms: A230067G21Rik, RGC2, AS250
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241694
Homologene: 28131
Iigp1
Name: interferon inducible GTPase 1
Synonyms: 2900074L10Rik, Irga6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 60440
VEGA: 18
Acss3
Name: acyl-CoA synthetase short-chain family member 3
Synonyms: LOC380660, 8430416H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 380660
Homologene: 11587
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Lrp4
Name: low density lipoprotein receptor-related protein 4
Synonyms: 6430526J12Rik, Megf7, mdig
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228357
HGNC: HGNC:6696
Homologene: 17964
Col19a1
Name: collagen, type XIX, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12823
HGNC: HGNC:2196
Homologene: 55608
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239420
Homologene: 65982
Egflam
Name: EGF-like, fibronectin type III and laminin G domains
Synonyms: nectican, pikachurin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 268780
Homologene: 65044
Grin2b
Name: glutamate receptor, ionotropic, NMDA2B (epsilon 2)
Synonyms: NMDAR2B, NR2B, Nmdar2b, GluRepsilon2, GluN2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14812
HGNC: HGNC:4586
Homologene: 646
Or10ak12
Name: olfactory receptor family 10 subfamily AK member 12
Synonyms: GA_x6K02T2QD9B-18726774-18727577, GA_x6K02T2QD9B-18723799-18724749, MOR259-5, MOR259-12, Olfr1335, Olfr1334-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 435804
Homologene: 138635
Golgb1
Name: golgi autoantigen, golgin subfamily b, macrogolgin 1
Synonyms: Giantin, C130074L01Rik, F730017E11Rik, 6330407A06Rik, Gm6840
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224139
HGNC: HGNC:4429
Homologene: 68401
Fam110c
Name: family with sequence similarity 110, member C
Synonyms: 9030611O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104943
VEGA: 12
Homologene: 75262
Kcnj2
Name: potassium inwardly-rectifying channel, subfamily J, member 2
Synonyms: IRK1, Kir2.1, Kcnf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16518
HGNC: HGNC:6263
Homologene: 20249
Or10n1
Name: olfactory receptor family 10 subfamily N member 1
Synonyms: M30, MOR224-4, GA_x6K02T2PVTD-33310486-33311418, Olfr148
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258498
VEGA: 9
Homologene: 128136
Or2n1
Name: olfactory receptor family 2 subfamily N member 1
Synonyms: MOR256-5, GA_x6K02T2PSCP-2623613-2624551, Olfr134
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258829
Homologene: 110603
Osbpl5
Name: oxysterol binding protein-like 5
Synonyms: Obph1, ORP5, 1110006M06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 79196
Homologene: 98024
Or6n1
Name: olfactory receptor family 6 subfamily N member 1
Synonyms: GA_x6K02T2P20D-21090094-21089156, MOR105-1, Olfr429
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258717
Homologene: 17366
Or10g1b
Name: olfactory receptor family 10 subfamily G member 1B
Synonyms: GA_x6K02T2RJGY-608749-609705, MOR223-9, Olfr1511
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258268
HGNC: HGNC:8170
Homologene: 87788
Lrrc14
Name: leucine rich repeat containing 14
Synonyms: E130306I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223664
Homologene: 40970
Brsk2
Name: BR serine/threonine kinase 2
Synonyms: SAD-A, 4833424K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75770
Homologene: 123829
Or8c15
Name: olfactory receptor family 8 subfamily C member 15
Synonyms: GA_x6K02T2PVTD-31889215-31890153, MOR170-11, Olfr893
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258333
Homologene: 133626
Ndufs3
Name: NADH:ubiquinone oxidoreductase core subunit S3
Synonyms: 0610010M09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68349
HGNC: HGNC:7710
Homologene: 3346
Donson
Name: downstream neighbor of SON
Synonyms: Donson, 1110025J21Rik, ORF60
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 60364
HGNC: HGNC:2993
Homologene: 32350
Zfp354c
Name: zinc finger protein 354C
Synonyms: Kid3, AJ18, 5330421P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 30944
Homologene: 56606
Aadacl4fm5
Name: AADACL4 family member 5
Synonyms: LOC329993, Gm438
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329993
Homologene: 135765
Lrrc3
Name: leucine rich repeat containing 3
Synonyms: 1300011L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237387
Homologene: 12789
Or7e165
Name: olfactory receptor family 7 subfamily E member 165
Synonyms: IG6, MOR146-7P, GA_x6K02T2PVTD-13523015-13523944, MOR146-3, Olfr58
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18358
Homologene: 134093
Slbp
Name: stem-loop binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20492
Homologene: 31389
Hspb1
Name: heat shock protein 1
Synonyms: Hsp25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 15507
HGNC: HGNC:5246
Homologene: 1180
Sult2a8
Name: sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 8
Synonyms: 2810007J24Rik, mL-STL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76971
Sh3tc2
Name: SH3 domain and tetratricopeptide repeats 2
Synonyms: D430044G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225608
VEGA: 18
Homologene: 11596
Trim31
Name: tripartite motif-containing 31
Synonyms: HCG1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224762
Homologene: 17137
Spata48
Name: spermatogenesis associated 48
Synonyms: 4930415F15Rik, post meiotic spermatogenesis 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 73862
Homologene: 87632
S100a3
Name: S100 calcium binding protein A3
Synonyms: S100E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20197
Homologene: 2223
Ttc39c
Name: tetratricopeptide repeat domain 39C
Synonyms: 1700008N02Rik, 2810439F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72747
Homologene: 124438
Pstk
Name: phosphoseryl-tRNA kinase
Synonyms: 5430423O14Rik, LOC381976, 5730458D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 214580
Homologene: 45167
Lce1h
Name: late cornified envelope 1H
Synonyms: 2310066F03Rik, Sprrl9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67718
Gm1988
Name: predicted gene 1988
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100038967
Homologene: 80005
Wbp4
Name: WW domain binding protein 4
Synonyms: FBP21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 22380
VEGA: 14
Homologene: 38287
Gm17041
Name: predicted gene 17041
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 24,293,112 bp
  • A to G, chromosome 1 at 170,004,729 bp
  • A to G, chromosome 1 at 174,089,975 bp
  • T to C, chromosome 2 at 80,512,690 bp
  • T to A, chromosome 2 at 90,902,346 bp
  • C to A, chromosome 2 at 91,497,073 bp
  • T to C, chromosome 2 at 146,342,510 bp
  • G to A, chromosome 3 at 59,263,213 bp
  • G to A, chromosome 3 at 90,601,823 bp
  • A to G, chromosome 3 at 92,763,720 bp
  • C to A, chromosome 4 at 53,042,381 bp
  • G to A, chromosome 4 at 118,809,363 bp
  • A to T, chromosome 4 at 144,778,113 bp
  • A to G, chromosome 5 at 33,248,527 bp
  • T to A, chromosome 5 at 33,641,913 bp
  • A to G, chromosome 5 at 101,661,832 bp
  • A to T, chromosome 5 at 135,889,332 bp
  • A to T, chromosome 6 at 118,654,372 bp
  • C to T, chromosome 6 at 135,736,306 bp
  • T to G, chromosome 7 at 14,425,514 bp
  • C to A, chromosome 7 at 29,845,433 bp
  • T to C, chromosome 7 at 39,171,638 bp
  • T to C, chromosome 7 at 100,121,353 bp
  • G to A, chromosome 7 at 131,374,036 bp
  • G to A, chromosome 7 at 141,998,473 bp
  • A to G, chromosome 7 at 143,741,696 bp
  • G to A, chromosome 8 at 12,926,646 bp
  • T to C, chromosome 8 at 33,574,171 bp
  • A to C, chromosome 8 at 71,511,451 bp
  • A to C, chromosome 9 at 19,783,865 bp
  • A to G, chromosome 9 at 38,209,754 bp
  • A to G, chromosome 9 at 39,613,620 bp
  • G to T, chromosome 9 at 107,300,606 bp
  • C to A, chromosome 10 at 77,901,465 bp
  • G to A, chromosome 10 at 94,789,738 bp
  • A to T, chromosome 10 at 106,937,147 bp
  • G to T, chromosome 10 at 118,209,461 bp
  • A to G, chromosome 10 at 128,241,735 bp
  • A to T, chromosome 11 at 11,490,244 bp
  • A to G, chromosome 11 at 17,212,031 bp
  • G to T, chromosome 11 at 50,815,770 bp
  • A to T, chromosome 11 at 55,497,987 bp
  • C to A, chromosome 11 at 59,000,128 bp
  • G to T, chromosome 11 at 111,072,231 bp
  • A to G, chromosome 12 at 31,074,407 bp
  • T to C, chromosome 12 at 75,493,476 bp
  • T to C, chromosome 12 at 83,776,350 bp
  • A to G, chromosome 13 at 14,187,696 bp
  • T to G, chromosome 14 at 29,986,995 bp
  • A to G, chromosome 14 at 52,390,125 bp
  • A to G, chromosome 14 at 65,054,626 bp
  • A to G, chromosome 14 at 67,022,323 bp
  • C to A, chromosome 14 at 79,472,397 bp
  • C to T, chromosome 15 at 7,224,663 bp
  • T to C, chromosome 15 at 48,791,931 bp
  • A to G, chromosome 15 at 59,330,904 bp
  • C to A, chromosome 15 at 76,715,600 bp
  • A to G, chromosome 16 at 36,900,508 bp
  • A to T, chromosome 16 at 91,679,926 bp
  • C to T, chromosome 17 at 33,886,665 bp
  • A to T, chromosome 17 at 36,905,905 bp
  • T to A, chromosome 17 at 38,176,026 bp
  • T to C, chromosome 17 at 57,204,502 bp
  • T to C, chromosome 18 at 4,675,790 bp
  • A to T, chromosome 18 at 12,695,371 bp
  • A to G, chromosome 18 at 60,390,257 bp
  • T to A, chromosome 18 at 61,989,633 bp
  • G to T, chromosome 19 at 27,412,259 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5439 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043004-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.