Strain Name:
C57BL/6J-MtgxR5440Btlr/Mmmh
Stock Number:
043005-MU
Citation ID:
RRID:MMRRC_043005-MU
Other Names:
R5440 (G1), C57BL/6J-MtgxR5440Btlr
Major Collection:

Gene Information

Secisbp2
Name: SECIS binding protein 2
Synonyms: 2810012K13Rik, SBP2, 2210413N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75420
Homologene: 11415
Syngr1
Name: synaptogyrin 1
Synonyms: p29, Syngr1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20972
Homologene: 3456
Cwf19l2
Name: CWF19-like 2, cell cycle control (S. pombe)
Synonyms: 3230401L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244672
Homologene: 12366
Dtx4
Name: deltex 4, E3 ubiquitin ligase
Synonyms: RNF155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 207521
VEGA: 19
Homologene: 27781
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Arhgap35
Name: Rho GTPase activating protein 35
Synonyms: p190RhoGAP, p190A, 6430596G11Rik, P190 RhoGAP, Grlf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232906
HGNC: HGNC:4591
Homologene: 35136
Prpf4b
Name: pre-mRNA processing factor 4B
Synonyms: Prpk, Prp4k, Prp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19134
VEGA: 13
Homologene: 134085
Prom1
Name: prominin 1
Synonyms: AC133, CD133, Prom, Prom-1, 4932416E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19126
HGNC: HGNC:9454
Homologene: 4390
Syt9
Name: synaptotagmin IX
Synonyms: Sytv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 60510
Homologene: 11062
Pikfyve
Name: phosphoinositide kinase, FYVE type zinc finger containing
Synonyms: 5230400C17Rik, PipkIII, Pip5k3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18711
Homologene: 32115
Sphk1
Name: sphingosine kinase 1
Synonyms: 1110006G24Rik, SK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20698
Homologene: 39748
Cpsf2
Name: cleavage and polyadenylation specific factor 2
Synonyms: 2610024B04Rik, 100kDa, Cpsf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 51786
VEGA: 12
HGNC: HGNC:2325
Homologene: 6460
Pigr
Name: polymeric immunoglobulin receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18703
HGNC: HGNC:8968
Homologene: 1984
Ube2d1
Name: ubiquitin-conjugating enzyme E2D 1
Synonyms: UBCH5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216080
Homologene: 20714
Havcr1
Name: hepatitis A virus cellular receptor 1
Synonyms: Tim1, KIM-1, TIM-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 171283
Homologene: 136301
Pamr1
Name: peptidase domain containing associated with muscle regeneration 1
Synonyms: RAMP, E430002G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 210622
Homologene: 9134
Apc
Name: APC, WNT signaling pathway regulator
Synonyms: adenomatosis polyposis coli, CC1, Min
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 11789
HGNC: HGNC:583
Homologene: 30950
Bcl9
Name: B cell CLL/lymphoma 9
Synonyms: A330041G23Rik, 8030475K17Rik, 2610202E01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77578
HGNC: HGNC:1008
Homologene: 3191
Slc35e2
Name: solute carrier family 35, member E2
Synonyms: A530082C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320541
Homologene: 84987
Ropn1
Name: ropporin, rhophilin associated protein 1
Synonyms: 1700008N21Rik, ODF6, ropporin, RHPNAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 76378
Homologene: 57119
Atp6v0a4
Name: ATPase, H+ transporting, lysosomal V0 subunit A4
Synonyms: Atp6n1b, V-ATPase alpha 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 140494
HGNC: HGNC:866
Homologene: 39904
Pkp1
Name: plakophilin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18772
HGNC: HGNC:9023
Homologene: 253
Ttn
Name: titin
Synonyms: D830007G01Rik, connectin, 1100001C23Rik, 2310036G12Rik, mdm, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru, L56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Pcsk2
Name: proprotein convertase subtilisin/kexin type 2
Synonyms: prohormone convertase 2, PC2, Nec-2, Nec2, SPC2, Phpp-2, 6330411F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18549
HGNC: HGNC:8744
Homologene: 37640
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Itgb4
Name: integrin beta 4
Synonyms: CD104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192897
HGNC: HGNC:6158
Homologene: 179
Cd109
Name: CD109 antigen
Synonyms: Gov platelet alloantigens, 9930012E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235505
Homologene: 25183
Grin2b
Name: glutamate receptor, ionotropic, NMDA2B (epsilon 2)
Synonyms: GluRepsilon2, Nmdar2b, GluN2B, NR2B, NMDAR2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14812
HGNC: HGNC:4586
Homologene: 646
Zfp770
Name: zinc finger protein 770
Synonyms: 6430601A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228491
Homologene: 82354
Olfr517
Name: olfactory receptor 517
Synonyms: MOR268-4, GA_x6K02T2PBJ9-11199311-11198367
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258136
Homologene: 79413
Gucy2e
Name: guanylate cyclase 2e
Synonyms: ROS-GC1, GC1, GC-E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14919
HGNC: HGNC:4689
Homologene: 55442
Myo5c
Name: myosin VC
Synonyms: 9130003O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208943
VEGA: 9
HGNC: HGNC:7604
Homologene: 135711
Lipo2
Name: lipase, member O2
Synonyms: Gm8981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 101055671
Homologene: 103863
P2rx1
Name: purinergic receptor P2X, ligand-gated ion channel, 1
Synonyms: Pdcd3, RP-2, P2X1 receptor, P2x
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18436
HGNC: HGNC:8533
Homologene: 1921
Hspa4l
Name: heat shock protein 4 like
Synonyms: 94kDa, Osp94, APG-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18415
Homologene: 22610
D7Ertd443e
Name: DNA segment, Chr 7, ERATO Doi 443, expressed
Synonyms: 4933400E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71007
Homologene: 18998
Fam186b
Name: family with sequence similarity 186, member B
Synonyms: EG545136
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 545136
VEGA: 15
Homologene: 69502
Ifna9
Name: interferon alpha 9
Synonyms: Ifa9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15972
HGNC: HGNC:5425
Homologene: 68536
Gzme
Name: granzyme E
Synonyms: CCP3, Ctla6, Ctla-6, MCSP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14942
VEGA: 14
Homologene: 133275
Terb1
Name: telomere repeat binding bouquet formation protein 1
Synonyms: Ccdc79, 4930532D21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320022
Homologene: 18330
Apol11b
Name: apolipoprotein L 11b
Synonyms: A330102K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 328563
Homologene: 129975
Fzd4
Name: frizzled class receptor 4
Synonyms: Fz4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14366
HGNC: HGNC:4042
Homologene: 7325
Prr33
Name: proline rich 33
Synonyms: Gm14492, ENSMUSG00000043795
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 677289
Hint3
Name: histidine triad nucleotide binding protein 3
Synonyms: HINT4, 0610010I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66847
Homologene: 12046
Gm1113
Name: predicted gene 1113
Synonyms: LOC382069
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Vmn2r41
Name: vomeronasal 2, receptor 41
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100042848
Homologene: 113703
Gm17669
Name: predicted gene, 17669
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 108168363
VEGA: 18
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 65,205,598 bp
  • A to T, chromosome 1 at 130,849,622 bp
  • A to T, chromosome 1 at 135,882,492 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • A to T, chromosome 2 at 76,909,256 bp
  • T to C, chromosome 2 at 102,614,936 bp
  • T to C, chromosome 2 at 114,196,115 bp
  • T to A, chromosome 2 at 143,546,543 bp
  • A to G, chromosome 3 at 40,781,576 bp
  • G to A, chromosome 3 at 97,210,565 bp
  • A to G, chromosome 4 at 88,591,811 bp
  • C to T, chromosome 4 at 155,610,026 bp
  • T to A, chromosome 5 at 44,058,646 bp
  • A to C, chromosome 6 at 38,092,817 bp
  • C to T, chromosome 6 at 135,736,306 bp
  • T to A, chromosome 7 at 8,138,363 bp
  • C to T, chromosome 7 at 16,562,924 bp
  • G to T, chromosome 7 at 89,408,118 bp
  • T to G, chromosome 7 at 107,502,123 bp
  • T to C, chromosome 7 at 108,868,626 bp
  • G to A, chromosome 7 at 134,349,275 bp
  • T to C, chromosome 7 at 142,492,483 bp
  • T to C, chromosome 8 at 104,488,499 bp
  • G to A, chromosome 9 at 3,475,549 bp
  • T to A, chromosome 9 at 35,516,896 bp
  • T to A, chromosome 9 at 75,258,125 bp
  • T to C, chromosome 9 at 78,680,164 bp
  • T to A, chromosome 9 at 79,614,363 bp
  • A to T, chromosome 10 at 30,618,351 bp
  • A to G, chromosome 10 at 71,255,852 bp
  • A to G, chromosome 10 at 76,723,454 bp
  • A to T, chromosome 11 at 46,752,370 bp
  • T to C, chromosome 11 at 69,223,646 bp
  • A to G, chromosome 11 at 73,008,503 bp
  • C to T, chromosome 11 at 115,984,157 bp
  • T to G, chromosome 11 at 116,534,888 bp
  • C to G, chromosome 12 at 101,996,879 bp
  • T to C, chromosome 13 at 34,884,093 bp
  • T to C, chromosome 13 at 51,662,680 bp
  • T to A, chromosome 14 at 56,118,453 bp
  • T to A, chromosome 15 at 77,635,593 bp
  • A to G, chromosome 15 at 80,098,018 bp
  • C to T, chromosome 15 at 99,273,853 bp
  • C to A, chromosome 16 at 34,671,172 bp
  • G to T, chromosome 18 at 34,221,160 bp
  • C to T, chromosome 18 at 67,562,456 bp
  • G to A, chromosome 19 at 12,492,317 bp
  • A to G, chromosome 19 at 33,720,858 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5440 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043005-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.