Strain Name:
C57BL/6J-MtgxR5444Btlr/Mmmh
Stock Number:
043009-MU
Citation ID:
RRID:MMRRC_043009-MU
Other Names:
R5444 (G1), C57BL/6J-MtgxR5444Btlr
Major Collection:

Gene Information

Gene Symbol: Nfatc2 [MGI:102463] (Mus musculus (mouse))
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1-D, NFAT1, NFATp
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Lamb1 [MGI:96743] (Mus musculus (mouse))
Name: laminin B1
Synonyms: D130003D08Rik, Lamb-1, Lamb1-1, C81607, C80098
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Cdk17 [MGI:97517] (Mus musculus (mouse))
Name: cyclin-dependent kinase 17
Synonyms: 6430598J10Rik, Pctk2
Chromosome: 10
Alteration at locus: Chemically Induced
Gene Symbol: Itgb8 [MGI:1338035] (Mus musculus (mouse))
Name: integrin beta 8
Synonyms: 4832412O06Rik
Chromosome: 12
Alteration at locus: Chemically Induced
Gene Symbol: Nnat [MGI:104716] (Mus musculus (mouse))
Name: neuronatin
Synonyms: Peg5, 5730414I02Rik
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Atic [MGI:1351352] (Mus musculus (mouse))
Name: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Synonyms: 2610509C24Rik
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Mpc1 [MGI:1915240] (Mus musculus (mouse))
Name: mitochondrial pyruvate carrier 1
Synonyms: 0610006G08Rik, 3830411I18Rik, Brp44l
Chromosome: 17
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 15,711,492 bp
  • G to T, chromosome 1 at 36,269,490 bp
  • T to C, chromosome 1 at 71,576,717 bp
  • T to A, chromosome 1 at 85,589,148 bp
  • C to T, chromosome 1 at 105,726,384 bp
  • T to C, chromosome 1 at 170,375,251 bp
  • A to C, chromosome 1 at 172,072,839 bp
  • A to T, chromosome 2 at 85,759,919 bp
  • T to C, chromosome 2 at 157,561,217 bp
  • A to G, chromosome 2 at 168,534,890 bp
  • T to A, chromosome 3 at 35,976,742 bp
  • T to C, chromosome 3 at 36,612,050 bp
  • T to C, chromosome 3 at 89,223,385 bp
  • T to C, chromosome 3 at 100,056,152 bp
  • A to G, chromosome 4 at 99,741,579 bp
  • T to A, chromosome 4 at 118,830,111 bp
  • G to A, chromosome 4 at 133,971,136 bp
  • A to G, chromosome 5 at 26,479,737 bp
  • A to G, chromosome 5 at 74,016,731 bp
  • T to C, chromosome 5 at 87,979,288 bp
  • A to T, chromosome 5 at 109,675,636 bp
  • G to A, chromosome 5 at 137,957,547 bp
  • A to T, chromosome 5 at 138,260,998 bp
  • C to T, chromosome 5 at 149,746,520 bp
  • T to A, chromosome 6 at 5,431,040 bp
  • T to A, chromosome 6 at 35,189,189 bp
  • A to T, chromosome 6 at 100,287,270 bp
  • A to T, chromosome 6 at 121,226,101 bp
  • T to C, chromosome 6 at 123,828,492 bp
  • A to T, chromosome 7 at 3,712,750 bp
  • A to T, chromosome 7 at 13,836,019 bp
  • T to C, chromosome 7 at 64,288,401 bp
  • T to A, chromosome 7 at 67,041,899 bp
  • T to C, chromosome 7 at 73,473,085 bp
  • T to A, chromosome 7 at 83,982,291 bp
  • C to T, chromosome 7 at 102,604,869 bp
  • G to C, chromosome 7 at 107,209,837 bp
  • G to A, chromosome 7 at 119,454,598 bp
  • C to T, chromosome 7 at 141,264,819 bp
  • T to C, chromosome 8 at 63,926,556 bp
  • A to G, chromosome 9 at 109,106,453 bp
  • T to A, chromosome 9 at 111,392,958 bp
  • T to G, chromosome 10 at 93,217,961 bp
  • A to G, chromosome 11 at 4,135,843 bp
  • T to C, chromosome 11 at 74,053,977 bp
  • T to C, chromosome 11 at 79,443,959 bp
  • C to T, chromosome 11 at 83,035,287 bp
  • T to C, chromosome 11 at 102,288,240 bp
  • A to G, chromosome 11 at 102,772,848 bp
  • G to A, chromosome 11 at 121,421,604 bp
  • T to C, chromosome 12 at 31,298,909 bp
  • T to A, chromosome 12 at 111,838,688 bp
  • T to C, chromosome 12 at 119,237,838 bp
  • T to C, chromosome 14 at 24,454,941 bp
  • T to C, chromosome 15 at 36,015,627 bp
  • T to C, chromosome 15 at 77,735,763 bp
  • G to T, chromosome 15 at 82,792,538 bp
  • T to A, chromosome 15 at 92,770,925 bp
  • G to A, chromosome 16 at 5,249,095 bp
  • A to G, chromosome 17 at 8,296,508 bp
  • A to G, chromosome 17 at 50,735,967 bp
  • A to T, chromosome 17 at 75,503,375 bp
  • A to T, chromosome 19 at 13,234,958 bp
  • A to G, chromosome 19 at 18,581,313 bp
  • A to G, chromosome 19 at 58,673,163 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5444 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043009-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.