Strain Name:
C57BL/6J-MtgxR5454Btlr/Mmmh
Stock Number:
043018-MU
Citation ID:
RRID:MMRRC_043018-MU
Other Names:
R5454 (G1), C57BL/6J-MtgxR5454Btlr
Major Collection:

Strain Information

Prkar1a
Name: protein kinase, cAMP dependent regulatory, type I, alpha
Synonyms: Tse1, Tse-1, 1300018C22Rik, RIalpha
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19084
HGNC: HGNC:9388
Homologene: 37664
Mga
Name: MAX gene associated
Synonyms: Mga, D030062C11Rik, C130042M01Rik, Mad5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Srgap2
Name: SLIT-ROBO Rho GTPase activating protein 2
Synonyms: 9930124L22Rik, FBP2, Fnbp2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14270
Homologene: 52683
Ints3
Name: integrator complex subunit 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229543
Homologene: 11309
Ankfy1
Name: ankyrin repeat and FYVE domain containing 1
Synonyms: Ankhzn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11736
Homologene: 9491
Utp6
Name: UTP6 small subunit processome component
Synonyms: HCA66, 4732497O03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216987
Homologene: 41265
Slc10a7
Name: solute carrier family 10 (sodium/bile acid cotransporter family), member 7
Synonyms: 2410193C02Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76775
Homologene: 81628
Ttk
Name: Ttk protein kinase
Synonyms: Esk1, Mps1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22137
Homologene: 2489
Kctd9
Name: potassium channel tetramerisation domain containing 9
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105440
Homologene: 9754
Dcaf13
Name: DDB1 and CUL4 associated factor 13
Synonyms: LOC223499, Wdsof1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223499
Homologene: 6430
Ccdc80
Name: coiled-coil domain containing 80
Synonyms: Urb, DRO1, Ssg1, 2610001E17Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67896
Homologene: 12206
Frzb
Name: frizzled-related protein
Synonyms: frzb-1, Frp, fritz, Sfrp3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20378
HGNC: HGNC:3959
Homologene: 1126
Tor1b
Name: torsin family 1, member B
Synonyms: torsinB, 2610016F05Rik, DQ1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 30934
Homologene: 56677
Apobec1
Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11810
HGNC: HGNC:604
Homologene: 1243
Pcx
Name: pyruvate carboxylase
Synonyms: Pc
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18563
VEGA: 19
HGNC: HGNC:8636
Homologene: 5422
Gcat
Name: glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase)
Synonyms: Kbl, aminoacetone synthase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 26912
VEGA: 15
HGNC: HGNC:4188
Homologene: 8475
Fgfr3
Name: fibroblast growth factor receptor 3
Synonyms: HBGFR, sam3, Fgfr-3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14184
HGNC: HGNC:3690
Homologene: 55437
Enpp7
Name: ectonucleotide pyrophosphatase/phosphodiesterase 7
Synonyms: LOC238011, Alk-SMase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 238011
Homologene: 110852
Pcmt1
Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase 1
Synonyms: PIMT, protein carboxyl methyltransferase
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18537
HGNC: HGNC:8728
Homologene: 135759
Gmds
Name: GDP-mannose 4, 6-dehydratase
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218138
HGNC: HGNC:4369
Homologene: 75968
Esco1
Name: establishment of sister chromatid cohesion N-acetyltransferase 1
Synonyms: A930014I12Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 77805
Homologene: 62166
Mtmr4
Name: myotubularin related protein 4
Synonyms: FYVE zinc finger phosphatase, ESTM44, ZFYVE11, FYVE-DSP2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 170749
HGNC: HGNC:7452
Homologene: 3440
Cd209b
Name: CD209b antigen
Synonyms: SIGNR1, mSIGNR1, 1810030I22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69165
Homologene: 84858
Pmch
Name: pro-melanin-concentrating hormone
Synonyms: melanin-concentrating hormone, MCH, A230109K23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 110312
Homologene: 37653
Strbp
Name: spermatid perinuclear RNA binding protein
Synonyms: 6430510M02Rik, Spnr, C230082I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20744
Homologene: 7548
Atp2b4
Name: ATPase, Ca++ transporting, plasma membrane 4
Synonyms: PMCA4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381290
HGNC: HGNC:817
Homologene: 48034
Sox6
Name: SRY (sex determining region Y)-box 6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20679
Homologene: 22631
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Itga9
Name: integrin alpha 9
Synonyms: 6720458D17Rik, 2610002H11Rik, D9Ertd428e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 104099
HGNC: HGNC:6145
Homologene: 1664
Plekhg4
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 4
Synonyms: 4931414L13Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102075
Homologene: 18516
Snrk
Name: SNF related kinase
Synonyms: SNRK, 2010012F07Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20623
Homologene: 9797
Nlrp9c
Name: NLR family, pyrin domain containing 9C
Synonyms: Nalp-zeta, Nalp9c
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330490
Homologene: 116072
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20192
Homologene: 68151
Synpo2l
Name: synaptopodin 2-like
Synonyms: Chap, 1110054M18Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68760
Homologene: 23499
Umodl1
Name: uromodulin-like 1
Synonyms: D17Ertd488e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 52020
VEGA: 17
Homologene: 45466
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 81877
Homologene: 49589
Usp13
Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
Synonyms: 2700071E21Rik, IsoT-3, ISOT3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72607
Homologene: 68372
Gtdc1
Name: glycosyltransferase-like domain containing 1
Synonyms: E330008O22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227835
Homologene: 32590
Tnrc18
Name: trinucleotide repeat containing 18
Synonyms: Zfp469, EG381742
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231861
Homologene: 45603
Itih2
Name: inter-alpha trypsin inhibitor, heavy chain 2
Synonyms: Itih-2, Intin2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16425
HGNC: HGNC:6167
Homologene: 1668
Zfp687
Name: zinc finger protein 687
Synonyms: 4931408L03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 78266
Homologene: 10827
Or5p54
Name: olfactory receptor family 5 subfamily P member 54
Synonyms: MOR204-20, GA_x6K02T2PBJ9-10283869-10284801, Olfr474
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258488
Dhcr7
Name: 7-dehydrocholesterol reductase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13360
HGNC: HGNC:2860
Homologene: 1042
Otud4
Name: OTU domain containing 4
Synonyms: D8Ertd69e, 4930431L18Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 73945
Homologene: 35370
Il5
Name: interleukin 5
Synonyms: Il-5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16191
HGNC: HGNC:6016
Homologene: 679
Ceacam14
Name: CEA cell adhesion molecule 14
Synonyms: 1600021E03Rik, 1600025E09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67084
Homologene: 135962
4930579C12Rik
Name: RIKEN cDNA 4930579C12 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319213
Cope
Name: coatomer protein complex, subunit epsilon
Synonyms: 1110005D17Rik, Cope1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 59042
HGNC: HGNC:2234
Homologene: 5254
Nckipsd
Name: NCK interacting protein with SH3 domain
Synonyms: WISH, ORF1, AF3P21, SPIN90, DIP1, Wasbp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 80987
Homologene: 9514
Vps45
Name: vacuolar protein sorting 45
Synonyms: mVps45
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 22365
Homologene: 5250
Gm13030
Name: predicted gene 13030
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 105734733
Htra2
Name: HtrA serine peptidase 2
Synonyms: Prss25, mnd2, HtrA2, OMI
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 64704
Homologene: 113300
Pcdhga12
Name: protocadherin gamma subfamily A, 12
Synonyms: Pcdh13, pc2c
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93724
HGNC: HGNC:8699
Homologene: 134588
Obox3-ps8
Name: oocyte specific homeobox 3, pseudogene 8
Synonyms: Gm4830
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224752
1700094J05Rik
Name: RIKEN cDNA 1700094J05 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67523
Loricrin
Name: loricrin cornified envelope precursor protein
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16939
HGNC: HGNC:6663
Ankrd46
Name: ankyrin repeat domain 46
Synonyms: 1110054N06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68839
VEGA: 15
Homologene: 27876
Pcdhgc3
Name: protocadherin gamma subfamily C, 3
Synonyms: Pcdh2, PC43
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93706
HGNC: HGNC:8716
Homologene: 31099
Zfp771
Name: zinc finger protein 771
Synonyms: G630024C07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244216
Homologene: 41151
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 131,289,737 bp
  • C to A, chromosome 1 at 133,729,872 bp
  • T to C, chromosome 2 at 10,097,993 bp
  • GGACG to GG, chromosome 2 at 30,956,945 bp
  • T to C, chromosome 2 at 37,645,483 bp
  • T to C, chromosome 2 at 44,565,502 bp
  • T to C, chromosome 2 at 80,417,915 bp
  • T to C, chromosome 2 at 112,730,302 bp
  • T to A, chromosome 2 at 119,903,329 bp
  • G to T, chromosome 3 at 32,905,436 bp
  • G to A, chromosome 3 at 90,408,527 bp
  • A to G, chromosome 3 at 92,081,482 bp
  • A to G, chromosome 3 at 95,009,146 bp
  • G to A, chromosome 3 at 96,019,657 bp
  • A to T, chromosome 4 at 138,873,509 bp
  • G to A, chromosome 5 at 33,723,298 bp
  • T to C, chromosome 5 at 142,771,691 bp
  • G to A, chromosome 6 at 83,054,014 bp
  • T to C, chromosome 6 at 122,581,368 bp
  • T to G, chromosome 7 at 17,814,185 bp
  • T to A, chromosome 7 at 26,385,712 bp
  • A to T, chromosome 7 at 107,954,889 bp
  • A to T, chromosome 7 at 115,701,773 bp
  • T to A, chromosome 7 at 127,254,276 bp
  • C to T, chromosome 7 at 141,648,813 bp
  • T to G, chromosome 7 at 143,837,839 bp
  • T to C, chromosome 8 at 3,925,396 bp
  • T to C, chromosome 8 at 70,304,656 bp
  • T to C, chromosome 8 at 78,686,624 bp
  • C to T, chromosome 8 at 79,651,042 bp
  • T to C, chromosome 8 at 105,376,113 bp
  • T to C, chromosome 9 at 83,843,740 bp
  • T to C, chromosome 9 at 89,168,988 bp
  • G to T, chromosome 9 at 108,815,028 bp
  • A to G, chromosome 9 at 118,698,372 bp
  • T to C, chromosome 9 at 122,166,299 bp
  • A to G, chromosome 10 at 7,640,745 bp
  • A to T, chromosome 10 at 76,389,547 bp
  • A to G, chromosome 10 at 88,091,845 bp
  • A to G, chromosome 11 at 53,723,799 bp
  • A to T, chromosome 11 at 72,746,931 bp
  • C to T, chromosome 11 at 79,959,127 bp
  • A to T, chromosome 11 at 87,611,042 bp
  • A to G, chromosome 11 at 109,660,060 bp
  • A to T, chromosome 11 at 118,988,808 bp
  • A to G, chromosome 13 at 32,128,041 bp
  • C to A, chromosome 14 at 20,662,292 bp
  • T to C, chromosome 14 at 67,740,387 bp
  • C to T, chromosome 15 at 36,479,301 bp
  • C to A, chromosome 15 at 39,124,364 bp
  • A to G, chromosome 15 at 79,036,410 bp
  • T to A, chromosome 16 at 45,127,225 bp
  • A to T, chromosome 17 at 30,986,465 bp
  • A to G, chromosome 17 at 34,709,625 bp
  • A to T, chromosome 17 at 36,453,011 bp
  • T to C, chromosome 18 at 10,584,327 bp
  • T to A, chromosome 18 at 37,766,261 bp
  • A to G, chromosome 18 at 37,808,496 bp
  • G to T, chromosome 19 at 4,602,476 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5454 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043018-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.