Strain Name:
C57BL/6J-MtgxR5472Btlr/Mmmh
Stock Number:
043033-MU
Citation ID:
RRID:MMRRC_043033-MU
Other Names:
R5472 (G1), C57BL/6J-MtgxR5472Btlr
Major Collection:

Strain Information

Tlr9
Name: toll-like receptor 9
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81897
Homologene: 68126
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Tns3
Name: tensin 3
Synonyms: TEM6, F830010I22Rik, Tens1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319939
Homologene: 49713
Sptan1
Name: spectrin alpha, non-erythrocytic 1
Synonyms: alpha-fodrin, Spna-2, 2610027H02Rik, Spna2, alphaII-spectrin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20740
Homologene: 2353
Odf2l
Name: outer dense fiber of sperm tails 2-like
Synonyms: 4733401D09Rik, 9630045K08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 52184
Homologene: 12011
Tsnax
Name: translin-associated factor X
Synonyms: Trax
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 53424
Homologene: 4374
S1pr3
Name: sphingosine-1-phosphate receptor 3
Synonyms: LPb3, S1P3, Edg3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13610
VEGA: 13
HGNC: HGNC:3167
Homologene: 3829
Cxcr4
Name: C-X-C motif chemokine receptor 4
Synonyms: PB-CKR, fusin, CD184, Cmkar4, Sdf1r, b2b220Clo
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12767
HGNC: HGNC:2561
Homologene: 20739
Lrsam1
Name: leucine rich repeat and sterile alpha motif containing 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227738
Homologene: 44526
Carmil1
Name: capping protein regulator and myosin 1 linker 1
Synonyms: 1110037D04Rik, Lrrc16, Carmil, Lrrc16a
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68732
Homologene: 9757
Lin9
Name: lin-9 DREAM MuvB core complex component
Synonyms: 2700022J23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72568
Homologene: 35252
Nup35
Name: nucleoporin 35
Synonyms: 5330402E05Rik, 2310006I24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69482
Homologene: 44517
Fancl
Name: Fanconi anemia, complementation group L
Synonyms: 2010322C19Rik, gcd, Pog, Phf9, B230118H11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67030
Homologene: 9987
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: 1200015F06Rik, Mypt1, D10Ertd625e, 5730577I22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855
Prpf8
Name: pre-mRNA processing factor 8
Synonyms: DBF3/PRP8, Prp8, D11Bwg0410e, Sfprp8l
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192159
Homologene: 4706
Mdh1
Name: malate dehydrogenase 1, NAD (soluble)
Synonyms: Mor-2, MDH-s, Mor2, B230377B03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17449
HGNC: HGNC:6970
Homologene: 4324
Msh6
Name: mutS homolog 6
Synonyms: Msh6, GTBP, Gtmbp
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17688
VEGA: 17
HGNC: HGNC:7329
Homologene: 149
Raf1
Name: v-raf-leukemia viral oncogene 1
Synonyms: Raf-1, 6430402F14Rik, v-Raf, sarcoma 3611 oncogene, c-Raf, Craf1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 110157
HGNC: HGNC:9829
Homologene: 48145
Gcnt2
Name: glucosaminyl (N-acetyl) transferase 2 (I blood group)
Synonyms: IGnTC, IGnTB, IGnTA, 5330430K10Rik, IGnT
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14538
VEGA: 13
HGNC: HGNC:4204
Homologene: 41535
Txndc5
Name: thioredoxin domain containing 5
Synonyms: PC-TRP, ERp46
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105245
Homologene: 11125
Heatr5b
Name: HEAT repeat containing 5B
Synonyms: D330050P16Rik, 2010013B10Rik, A230048G03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320473
VEGA: 17
Homologene: 25536
Akap11
Name: A kinase anchor protein 11
Synonyms: 6330501D17Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219181
HGNC: HGNC:369
Homologene: 8279
Jag1
Name: jagged 1
Synonyms: Serrate-1, Headturner, Htu, ABE2, Ozz, Gsfabe2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16449
HGNC: HGNC:6188
Homologene: 180
Ercc5
Name: excision repair cross-complementing rodent repair deficiency, complementation group 5
Synonyms: Xpg
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22592
HGNC: HGNC:3437
Homologene: 133551
Cacna1c
Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
Synonyms: (alpha)1 subunit, Cchl1a1, Cav1.2, L-type Cav1.2, D930026N18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12288
HGNC: HGNC:1390
Homologene: 55484
Cspg4b
Name: chondroitin sulfate proteoglycan 4B
Synonyms: BC067074
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 408066
Eif3f
Name: eukaryotic translation initiation factor 3, subunit F
Synonyms: 0610037M02Rik, Eif3s5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66085
HGNC: HGNC:3275
Homologene: 2783
Inhba
Name: inhibin beta-A
Synonyms: activin beta-A
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16323
VEGA: 13
HGNC: HGNC:6066
Homologene: 1653
Brinp3
Name: bone morphogenetic protein/retinoic acid inducible neural specific 3
Synonyms: B830045N13Rik, Fam5c
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 215378
Homologene: 17786
Madd
Name: MAP-kinase activating death domain
Synonyms: 9630059K23Rik, Rab3 GEP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228355
HGNC: HGNC:6766
Homologene: 14249
Slx4
Name: SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae)
Synonyms: D16Bwg1016e, Btbd12
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 52864
Homologene: 23770
Dpp7
Name: dipeptidylpeptidase 7
Synonyms: QPP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 83768
Homologene: 22748
Rasal3
Name: RAS protein activator like 3
Synonyms: A430107D22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320484
Homologene: 18901
Vwde
Name: von Willebrand factor D and EGF domains
Synonyms: LOC232585
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232585
Homologene: 35456
Gm973
Name: predicted gene 973
Synonyms: LOC381260
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381260
Homologene: 124277
Kcna2
Name: potassium voltage-gated channel, shaker-related subfamily, member 2
Synonyms: Kv1.2, Kca1-2, Mk-2, Akr6a4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16490
HGNC: HGNC:6220
Homologene: 21034
Sp7
Name: Sp7 transcription factor 7
Synonyms: Osx, 6430578P22Rik, osterix
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170574
VEGA: 15
Homologene: 15607
Col16a1
Name: collagen, type XVI, alpha 1
Synonyms: [a]1 (XVI) collagen, 2700007F12Rik, A530052M23Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 107581
HGNC: HGNC:2193
Homologene: 1397
Pcolce
Name: procollagen C-endopeptidase enhancer protein
Synonyms: Astt-2, Astt2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18542
HGNC: HGNC:8738
Homologene: 1946
Ube2q1
Name: ubiquitin-conjugating enzyme E2Q family member 1
Synonyms: 1110002C01Rik, PRO3094, NICE-5, 2310012M18Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70093
Homologene: 9730
Pramel15
Name: PRAME like 15
Synonyms: EG627009, Gm13125, Pramef20
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 627009
Homologene: 133194
Kcnh8
Name: potassium voltage-gated channel, subfamily H (eag-related), member 8
Synonyms: Kv12.1, ELK1, C130090D05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 211468
Homologene: 14332
Vmn2r28
Name: vomeronasal 2, receptor 28
Synonyms: EG665255
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 665255
Homologene: 129605
Or6c69c
Name: olfactory receptor family 6 subfamily C member 69C
Synonyms: GA_x6K02T2PULF-11745102-11746040, MOR113-2, Olfr822
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258666
Homologene: 133890
Zfp109
Name: zinc finger protein 109
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56869
Wdr35
Name: WD repeat domain 35
Synonyms: 4930459M12Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 74682
Homologene: 10814
Setd7
Name: SET domain containing (lysine methyltransferase) 7
Synonyms: 1600028F23Rik, Set7, KMT7, Set7/9
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 73251
Homologene: 12741
Ajm1
Name: apical junction component 1
Synonyms: LOC381353, Gm996
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 381353
Homologene: 19943
Tmem117
Name: transmembrane protein 117
Synonyms: B930062P21Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 320709
VEGA: 15
Homologene: 12992
Mdfic2
Name: MyoD family inhibitor domain containing 2
Synonyms: LOC330390, Gm765
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330390
Homologene: 66631
Mrps22
Name: mitochondrial ribosomal protein S22
Synonyms: 3100002P07Rik, Rpms22
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 64655
Homologene: 57030
Tmem45b
Name: transmembrane protein 45b
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235135
VEGA: 9
Homologene: 16349
Ifi213
Name: interferon activated gene 213
Synonyms: E030037K03Rik, Pydc4, Pyr-A
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 623121
HGNC: HGNC:5395
Homologene: 115929
Cdk2ap2
Name: cyclin dependent kinase 2 associated protein 2
Synonyms: 5830466O21Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 52004
Homologene: 4272
Gm10044
Name: predicted gene 10044
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218695
Homologene: 141165
Ighv1-20
Name: immunoglobulin heavy variable V1-20
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 668497
HGNC: HGNC:5553
Nlrp5-ps
Name: NLR family, pyrin domain containing 5, pseudogene
Synonyms: Gm18756
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100417675
Irx3os
Name: iroquois homeobox 3, opposite strand
Synonyms: D230002A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319388
Gm11733
Name: predicted gene 11733
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 432615
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 44,180,872 bp
  • A to G, chromosome 1 at 59,628,287 bp
  • C to A, chromosome 1 at 128,589,625 bp
  • A to T, chromosome 1 at 146,901,459 bp
  • A to T, chromosome 1 at 173,567,272 bp
  • A to G, chromosome 1 at 180,688,382 bp
  • A to G, chromosome 2 at 25,353,751 bp
  • T to C, chromosome 2 at 25,579,702 bp
  • T to A, chromosome 2 at 30,030,423 bp
  • ACC to AC, chromosome 2 at 32,945,858 bp
  • C to T, chromosome 2 at 80,658,401 bp
  • G to A, chromosome 2 at 91,162,730 bp
  • C to A, chromosome 2 at 137,084,995 bp
  • G to A, chromosome 3 at 51,521,465 bp
  • A to G, chromosome 3 at 89,777,241 bp
  • T to C, chromosome 3 at 107,105,309 bp
  • G to T, chromosome 3 at 145,146,866 bp
  • T to C, chromosome 4 at 123,450,061 bp
  • T to C, chromosome 4 at 130,092,771 bp
  • T to C, chromosome 4 at 144,377,157 bp
  • G to A, chromosome 5 at 137,605,581 bp
  • A to T, chromosome 6 at 13,193,118 bp
  • A to T, chromosome 6 at 98,238,276 bp
  • A to G, chromosome 6 at 115,626,706 bp
  • A to G, chromosome 6 at 118,638,446 bp
  • C to T, chromosome 7 at 5,487,944 bp
  • G to A, chromosome 7 at 14,580,975 bp
  • A to T, chromosome 7 at 24,228,621 bp
  • A to G, chromosome 7 at 108,938,396 bp
  • G to T, chromosome 8 at 91,799,480 bp
  • A to G, chromosome 8 at 125,015,762 bp
  • T to A, chromosome 9 at 31,428,044 bp
  • C to T, chromosome 9 at 98,598,122 bp
  • T to A, chromosome 9 at 106,224,313 bp
  • C to T, chromosome 10 at 108,240,112 bp
  • A to T, chromosome 10 at 130,075,029 bp
  • A to G, chromosome 11 at 8,451,092 bp
  • A to T, chromosome 11 at 21,559,786 bp
  • G to T, chromosome 11 at 26,469,677 bp
  • A to C, chromosome 11 at 75,503,643 bp
  • A to T, chromosome 11 at 117,484,496 bp
  • T to A, chromosome 12 at 9,016,619 bp
  • A to T, chromosome 12 at 114,723,851 bp
  • A to G, chromosome 13 at 16,026,786 bp
  • C to T, chromosome 13 at 24,155,471 bp
  • G to A, chromosome 13 at 38,513,125 bp
  • T to C, chromosome 13 at 40,953,579 bp
  • T to A, chromosome 13 at 51,419,647 bp
  • A to G, chromosome 13 at 113,319,169 bp
  • A to G, chromosome 14 at 7,791,483 bp
  • T to C, chromosome 14 at 78,513,429 bp
  • T to A, chromosome 15 at 95,094,513 bp
  • G to A, chromosome 15 at 102,359,314 bp
  • C to T, chromosome 16 at 3,991,540 bp
  • C to A, chromosome 17 at 32,396,669 bp
  • A to T, chromosome 17 at 52,977,816 bp
  • A to T, chromosome 17 at 78,801,660 bp
  • G to A, chromosome 17 at 87,984,561 bp
  • C to A, chromosome 19 at 4,098,048 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5472 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043033-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.