Strain Name:
C57BL/6J-MtgxR5483Btlr/Mmmh
Stock Number:
043044-MU
Citation ID:
RRID:MMRRC_043044-MU
Other Names:
R5483 (G1), C57BL/6J-MtgxR5483Btlr
Major Collection:

Gene Information

Dnmt3a
Name: DNA methyltransferase 3A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 13435
HGNC: HGNC:2978
Homologene: 7294
Usp7
Name: ubiquitin specific peptidase 7
Synonyms: 2210010O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 252870
Homologene: 2592
Scrib
Name: scribbled planar cell polarity
Synonyms: Crc, Scrb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 105782
Homologene: 44228
Hira
Name: histone cell cycle regulator
Synonyms: Tuple1, Gm15797, D16Ertd95e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 15260
HGNC: HGNC:4916
Homologene: 48172
Ociad1
Name: OCIA domain containing 1
Synonyms: Imi2, Asrij, 6030432N09Rik, Emi2, TPA018, B230209J16Rik, expressed during mesenchymal induction 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 68095
Homologene: 9866
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: Mll, Mll1, All1, HTRX1, ALL-1, trithorax Drosophila, Cxxc7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Srrm2
Name: serine/arginine repetitive matrix 2
Synonyms: 5033413A03Rik, SRm300
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 75956
Homologene: null
Heatr1
Name: HEAT repeat containing 1
Synonyms: B130016L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 217995
VEGA: 13
Homologene: 34562
Ipo5
Name: importin 5
Synonyms: 1110011C18Rik, RanBP5, 5730478E03Rik, Kpnb3, IMB3, importin beta 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 70572
VEGA: 14
HGNC: HGNC:6402
Homologene: 1710
Vim
Name: vimentin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22352
Homologene: 2538
Rffl
Name: ring finger and FYVE like domain containing protein
Synonyms: Carp-2, 4930516L10Rik, 1700051E09Rik, rififylin, fring, Carp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 67338
Homologene: 12116
Vps39
Name: VPS39 HOPS complex subunit
Synonyms: Vam6, Vam6P, A230065P22Rik, mVam6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 269338
Homologene: 41025
Slc25a40
Name: solute carrier family 25, member 40
Synonyms: B230315F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 319653
Homologene: 6040
Dnttip2
Name: deoxynucleotidyltransferase, terminal, interacting protein 2
Synonyms: 4930588M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99480
Homologene: 124162
Mlh1
Name: mutL homolog 1
Synonyms: colon cancer, nonpolyposis type 2, 1110035C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17350
HGNC: HGNC:7127
Homologene: 208
Slc22a3
Name: solute carrier family 22 (organic cation transporter), member 3
Synonyms: Orct3, Oct3, EMT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 20519
VEGA: 17
Homologene: 22630
Pole
Name: polymerase (DNA directed), epsilon
Synonyms: pol-epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 18973
HGNC: HGNC:9177
Homologene: 4539
Lmbrd1
Name: LMBR1 domain containing 1
Synonyms: 0910001K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 68421
Homologene: 10156
Emc1
Name: ER membrane protein complex subunit 1
Synonyms: C230096C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230866
Homologene: 9002
Nbeal1
Name: neurobeachin like 1
Synonyms: ALS2CR17, 2310076G13Rik, A530083I02Rik, A530050O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 269198
Homologene: 16453
Pkd1l1
Name: polycystic kidney disease 1 like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 171395
Homologene: 51376
Rasgrp3
Name: RAS, guanyl releasing protein 3
Synonyms: LOC240168
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 240168
VEGA: 17
Homologene: 15019
Cfap206
Name: cilia and flagella associated protein 206
Synonyms: 1700003M02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69329
Homologene: 18713
Fbxo24
Name: F-box protein 24
Synonyms: 4933422D21Rik, Fbx24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 71176
Homologene: 14131
Enkur
Name: enkurin, TRPC channel interacting protein
Synonyms: 4933434I06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 71233
Homologene: 17022
Olfr323
Name: olfactory receptor 323
Synonyms: GA_x6K02T2NKPP-794386-795357, MOR107-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 258373
Homologene: 64865
Kctd16
Name: potassium channel tetramerisation domain containing 16
Synonyms: 4930434H12Rik, 2900055J20Rik, LOC383347
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 73001
VEGA: 18
Homologene: 44779
Klf11
Name: Kruppel-like factor 11
Synonyms: Tieg2b, Tieg2, Tieg3, D12Ertd427e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 194655
Homologene: 2668
Serpinb3a
Name: serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 3A
Synonyms: Sqn5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20248
Homologene: 76659
Sirpb1b
Name: signal-regulatory protein beta 1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 668101
Homologene: 82993
Olfr784
Name: olfactory receptor 784
Synonyms: GA_x6K02T2PULF-11068246-11069205, MOR110-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 258724
HGNC: null
Homologene: 138306
Socs5
Name: suppressor of cytokine signaling 5
Synonyms: 1810018L08Rik, Cish5, SOCS-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 56468
VEGA: 17
Homologene: 10495
Polh
Name: polymerase (DNA directed), eta (RAD 30 related)
Synonyms: RAD30A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 80905
VEGA: 17
HGNC: HGNC:9181
Homologene: 38189
Prss29
Name: protease, serine 29
Synonyms: mISP-2, Isp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 114662
VEGA: 17
Homologene: 70877
Pate13
Name: prostate and testis expressed 13
Synonyms: 9230113P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 77908
HGNC: null
Homologene: null
Olfr1038-ps
Name: olfactory receptor 1038, pseudogene
Synonyms: GA_x6K02T2Q125-47591072-47592031, MOR185-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 259015
Homologene: 17463
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 24,744,908 bp
  • G to A, chromosome 1 at 60,283,983 bp
  • T to A, chromosome 1 at 107,047,169 bp
  • T to G, chromosome 2 at 13,574,605 bp
  • A to T, chromosome 2 at 21,194,298 bp
  • C to T, chromosome 2 at 86,122,618 bp
  • A to T, chromosome 2 at 120,323,083 bp
  • T to C, chromosome 3 at 15,543,246 bp
  • A to T, chromosome 3 at 122,276,797 bp
  • G to T, chromosome 4 at 34,711,404 bp
  • A to G, chromosome 4 at 139,375,376 bp
  • A to T, chromosome 5 at 8,427,449 bp
  • T to C, chromosome 5 at 73,294,971 bp
  • T to A, chromosome 5 at 110,294,568 bp
  • C to T, chromosome 5 at 137,618,740 bp
  • A to G, chromosome 9 at 35,909,584 bp
  • A to T, chromosome 9 at 44,824,624 bp
  • G to A, chromosome 9 at 111,231,058 bp
  • T to C, chromosome 10 at 129,387,657 bp
  • C to T, chromosome 11 at 8,901,141 bp
  • T to A, chromosome 11 at 58,625,957 bp
  • G to T, chromosome 11 at 82,812,723 bp
  • A to G, chromosome 12 at 3,899,615 bp
  • T to A, chromosome 12 at 24,655,411 bp
  • A to G, chromosome 13 at 12,398,914 bp
  • T to A, chromosome 14 at 120,920,038 bp
  • A to G, chromosome 15 at 76,067,659 bp
  • T to A, chromosome 16 at 8,698,540 bp
  • T to A, chromosome 16 at 18,969,540 bp
  • C to T, chromosome 17 at 12,464,467 bp
  • T to A, chromosome 17 at 23,821,272 bp
  • A to G, chromosome 17 at 25,322,203 bp
  • G to T, chromosome 17 at 46,172,745 bp
  • T to G, chromosome 17 at 75,525,018 bp
  • T to G, chromosome 17 at 87,134,974 bp
  • A to G, chromosome 18 at 40,530,876 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5483 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043044-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.