Strain Name:
C57BL/6J-MtgxR5499Btlr/Mmmh
Stock Number:
043060-MU
Citation ID:
RRID:MMRRC_043060-MU
Other Names:
R5499 (G1), C57BL/6J-MtgxR5499Btlr
Major Collection:

Gene Information

Lrig2
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 269473
Homologene: 8882
Fn1
Name: fibronectin 1
Synonyms: Fn, Fn-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14268
HGNC: HGNC:3778
Homologene: 1533
Asf1a
Name: anti-silencing function 1A histone chaperone
Synonyms: 2310079C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66403
VEGA: 10
Homologene: 8528
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77595
Homologene: 28122
Ppp2r3c
Name: protein phosphatase 2, regulatory subunit B'', gamma
Synonyms: G5pr, G4-1, 5730412A08Rik, 4930511A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 59032
VEGA: 12
Homologene: 9915
Zfp101
Name: zinc finger protein 101
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22643
Homologene: 137226
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, Phr1, C130061D10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Zfp609
Name: zinc finger protein 609
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214812
Homologene: 72220
Anapc5
Name: anaphase-promoting complex subunit 5
Synonyms: 2510006G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 59008
Homologene: 41118
Cep290
Name: centrosomal protein 290
Synonyms: b2b1454Clo, b2b1752Clo, Kiaa, Nphp6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Ercc6
Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
Synonyms: CS group B correcting gene, C130058G22Rik, CSB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 319955
VEGA: 14
HGNC: HGNC:3438
Homologene: 133552
Vrk1
Name: vaccinia related kinase 1
Synonyms: 51PK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 22367
VEGA: 12
Homologene: 2541
Top2a
Name: topoisomerase (DNA) II alpha
Synonyms: DNA Topoisomerase II alpha, Top-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21973
Homologene: 830
Lamb2
Name: laminin, beta 2
Synonyms: Lams, Lamb-2, npht
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16779
HGNC: HGNC:6487
Homologene: 1723
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: Duplin, 5830451P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67772
Homologene: 72405
Sgcb
Name: sarcoglycan, beta (dystrophin-associated glycoprotein)
Synonyms: beta-SG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 24051
Homologene: 195
Kif13a
Name: kinesin family member 13A
Synonyms: 4930505I07Rik, N-3 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16553
VEGA: 13
Homologene: 22589
Ino80
Name: INO80 complex subunit
Synonyms: INO80, 2310079N15Rik, Inoc1, 4632409L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68142
Homologene: 75070
Vps51
Name: VPS51 GARP complex subunit
Synonyms: 3110057M17Rik, 1110014N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 68505
HGNC: HGNC:1172
Homologene: 34675
Hnrnpa3
Name: heterogeneous nuclear ribonucleoprotein A3
Synonyms: 2410013L13Rik, 2610510D13Rik, 2610209F03Rik, Hnrpa3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 229279
Homologene: 138303
Cyb5r1
Name: cytochrome b5 reductase 1
Synonyms: 1500005G05Rik, Nqo3a2, B5R.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72017
Homologene: 96059
Zcchc2
Name: zinc finger, CCHC domain containing 2
Synonyms: 9930114B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227449
Homologene: 9808
Zfc3h1
Name: zinc finger, C3H1-type containing
Synonyms: Ccdc131, Psrc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216345
Homologene: 17017
Tlr3
Name: toll-like receptor 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 142980
Homologene: 20696
Lct
Name: lactase
Synonyms: LPH, Lphl, LOC226413
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226413
HGNC: HGNC:6530
Homologene: 124204
Slc45a2
Name: solute carrier family 45, member 2
Synonyms: Aim-1, Oca4, bls, Dbr, Aim1, Matp, dominant brown, blanc-sale
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22293
Homologene: 9412
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: b2b1554Clo, CD91, A2mr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: Dnahc11, avc4, b2b598Clo, lrd, b2b1289Clo, b2b1727Clo, b2b1279Clo, b2b1203Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Tcea2
Name: transcription elongation factor A (SII), 2
Synonyms: Tceat, S-II-T1, SII-T1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21400
Homologene: 68304
Casc1
Name: cancer susceptibility candidate 1
Synonyms: A230084G12Rik, Las1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320662
Homologene: 41242
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242627
Homologene: 135888
Fbxl4
Name: F-box and leucine-rich repeat protein 4
Synonyms: FBL5, FBL4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269514
Homologene: 8128
Ptafr
Name: platelet-activating factor receptor
Synonyms: PAF receptor, PAFR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19204
HGNC: HGNC:9582
Homologene: 20260
Rpgrip1
Name: retinitis pigmentosa GTPase regulator interacting protein 1
Synonyms: A930002K18Rik, nmf247, 4930401L23Rik, 4930505G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 77945
Homologene: 10679
Mmp12
Name: matrix metallopeptidase 12
Synonyms: MMP12, Mmel, macrophage elastase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17381
HGNC: HGNC:7158
Homologene: 20547
Ctse
Name: cathepsin E
Synonyms: CE, CatE, A430072O03Rik, C920004C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13034
HGNC: HGNC:2530
Homologene: 37551
Myocd
Name: myocardin
Synonyms: Srfcp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 214384
Homologene: 17043
Stk-ps2
Name: serine/threonine kinase 2
Synonyms: Gm4776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 212225
Palmd
Name: palmdelphin
Synonyms: 4631423C22Rik, PALML
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 114301
Homologene: 9804
Adam10
Name: a disintegrin and metallopeptidase domain 10
Synonyms: kuz, kuzbanian, 1700031C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11487
HGNC: HGNC:188
Homologene: 865
Klk1b21
Name: kallikrein 1-related peptidase b21
Synonyms: mGk-21, Klk21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16616
HGNC: HGNC:6364
Homologene: 68141
Phtf1
Name: putative homeodomain transcription factor 1
Synonyms: Phft
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18685
HGNC: HGNC:8939
Homologene: 4817
4933409G03Rik
Name: RIKEN cDNA 4933409G03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227998
Slfn8
Name: schlafen 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 276950
Homologene: 45432
Atxn1l
Name: ataxin 1-like
Synonyms: C330011L24Rik, D8Ertd587e, Boat, ENSMUSG00000069895
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 52335
Homologene: 19471
Fstl4
Name: follistatin-like 4
Synonyms: B230374F23Rik, SPIG1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 320027
Homologene: 18543
Olfr748
Name: olfactory receptor 748
Synonyms: GA_x6K02T2PMLR-6454789-6455712, MOR106-9P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258113
Homologene: 134081
Olfr366
Name: olfactory receptor 366
Synonyms: GA_x6K02T2NLDC-33902472-33903401, MOR138-5P, MOR138-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 236509
Homologene: 114744
H2-Q7
Name: histocompatibility 2, Q region locus 7
Synonyms: Ped, Qa-7, H-2Q7, Qa7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15018
HGNC: HGNC:4931
Homologene: 128352
Galnt17
Name: polypeptide N-acetylgalactosaminyltransferase 17
Synonyms: Wbscr17, Gcap8, E330012B09Rik, Galnt19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 212996
Homologene: 49707
Olfr675
Name: olfactory receptor 675
Synonyms: GA_x6K02T2PBJ9-7653782-7652841, MOR32-9P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258147
Homologene: 133595
Herpud1
Name: homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
Synonyms: Herp, Mifl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 64209
Homologene: 40973
Diras2
Name: DIRAS family, GTP-binding RAS-like 2
Synonyms: 2900052J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68203
VEGA: 13
Homologene: 56777
Gm14471
Name: predicted gene 14471
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 46,030,224 bp
  • A to G, chromosome 1 at 71,618,658 bp
  • C to T, chromosome 1 at 106,030,592 bp
  • T to A, chromosome 1 at 128,286,677 bp
  • T to A, chromosome 1 at 131,672,513 bp
  • T to C, chromosome 1 at 134,408,500 bp
  • T to A, chromosome 2 at 30,322,589 bp
  • T to C, chromosome 2 at 37,219,765 bp
  • G to A, chromosome 2 at 68,601,793 bp
  • A to G, chromosome 2 at 75,665,240 bp
  • A to T, chromosome 2 at 119,441,647 bp
  • A to G, chromosome 2 at 181,680,434 bp
  • T to C, chromosome 3 at 90,174,370 bp
  • T to A, chromosome 3 at 103,991,175 bp
  • T to A, chromosome 3 at 104,461,557 bp
  • T to A, chromosome 3 at 116,923,832 bp
  • A to G, chromosome 4 at 22,386,017 bp
  • A to G, chromosome 4 at 113,942,503 bp
  • A to G, chromosome 4 at 132,579,335 bp
  • T to C, chromosome 5 at 73,644,405 bp
  • C to T, chromosome 5 at 122,788,413 bp
  • T to A, chromosome 5 at 130,900,628 bp
  • A to T, chromosome 6 at 145,177,431 bp
  • A to G, chromosome 7 at 44,105,676 bp
  • A to T, chromosome 7 at 105,024,977 bp
  • C to T, chromosome 8 at 45,398,814 bp
  • C to T, chromosome 8 at 94,389,413 bp
  • A to G, chromosome 8 at 109,731,632 bp
  • C to A, chromosome 9 at 7,353,000 bp
  • A to G, chromosome 9 at 65,702,855 bp
  • A to T, chromosome 9 at 70,740,117 bp
  • T to C, chromosome 9 at 108,487,802 bp
  • T to A, chromosome 10 at 53,606,170 bp
  • G to A, chromosome 10 at 100,537,653 bp
  • T to A, chromosome 10 at 115,410,693 bp
  • C to T, chromosome 10 at 127,572,944 bp
  • G to T, chromosome 11 at 53,068,547 bp
  • A to G, chromosome 11 at 65,178,749 bp
  • C to A, chromosome 11 at 83,004,216 bp
  • C to T, chromosome 11 at 99,022,376 bp
  • T to C, chromosome 12 at 55,288,626 bp
  • A to G, chromosome 12 at 106,051,765 bp
  • A to T, chromosome 12 at 118,106,474 bp
  • A to G, chromosome 13 at 46,832,736 bp
  • C to T, chromosome 13 at 52,507,750 bp
  • G to A, chromosome 14 at 32,516,959 bp
  • G to A, chromosome 14 at 50,710,867 bp
  • T to A, chromosome 14 at 52,140,585 bp
  • A to G, chromosome 14 at 52,204,431 bp
  • T to C, chromosome 14 at 103,242,179 bp
  • C to T, chromosome 15 at 11,027,785 bp
  • T to C, chromosome 17 at 33,382,344 bp
  • T to A, chromosome 17 at 35,439,940 bp
  • T to G, chromosome 19 at 6,071,033 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5499 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043060-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.