Strain Name:
C57BL/6J-MtgxR5520Btlr/Mmmh
Stock Number:
043079-MU
Citation ID:
RRID:MMRRC_043079-MU
Other Names:
R5520 (G1), C57BL/6J-MtgxR5520Btlr
Major Collection:

Gene Information

Ulk2
Name: unc-51 like kinase 2
Synonyms: Unc51.2, A830085I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 29869
Homologene: 5891
E2f7
Name: E2F transcription factor 7
Synonyms: D10Ertd739e, E2F7, A630014C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 52679
Homologene: 18685
Cep97
Name: centrosomal protein 97
Synonyms: 4932439K18Rik, 2810403B08Rik, E130116N02Rik, Lrriq2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 74201
Homologene: 11579
Sfswap
Name: splicing factor SWAP
Synonyms: Sfrs8, 6330437E22Rik, 1190005N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231769
Homologene: 134548
Cc2d1b
Name: coiled-coil and C2 domain containing 1B
Synonyms: Freud2, A830039B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 319965
Homologene: 70951
Trim23
Name: tripartite motif-containing 23
Synonyms: 6330516O20Rik, Arfd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 81003
HGNC: HGNC:660
Homologene: 1251
Tmem245
Name: transmembrane protein 245
Synonyms: A630051L19Rik, D730040F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242474
HGNC: HGNC:1363
Homologene: 41841
Dclk2
Name: doublecortin-like kinase 2
Synonyms: Dcamkl2, Click-II, 6330415M09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 70762
Homologene: 69431
Lepr
Name: leptin receptor
Synonyms: Leprb, LEPROT, Modb1, OB-RGRP, leptin receptor gene-related protein, obl, obese-like, Obr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 16847
HGNC: HGNC:6554
Homologene: 1731
Plxnb2
Name: plexin B2
Synonyms: 1110007H23Rik, Debt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 140570
HGNC: HGNC:9104
Homologene: 66630
Eya3
Name: EYA transcriptional coactivator and phosphatase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 14050
HGNC: HGNC:3521
Homologene: 1508
Unkl
Name: unkempt family like zinc finger
Synonyms: 1300004G08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 74154
Homologene: 62673
Zfp534
Name: zinc finger protein 534
Synonyms: Gm13159
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100503584
Homologene: 133076
Nphp3
Name: nephronophthisis 3 (adolescent)
Synonyms: nephrocystin 3, pcy, D330020E01Rik, 3632410F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 74025
HGNC: HGNC:7907
Homologene: 32697
Grk1
Name: G protein-coupled receptor kinase 1
Synonyms: Rhok, RK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 24013
Homologene: 2197
AI467606
Name: expressed sequence AI467606
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 101602
Homologene: 18492
C87499
Name: expressed sequence C87499
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 381590
Ftsj3
Name: FtsJ RNA methyltransferase homolog 3 (E. coli)
Synonyms: C79843, Epcs3, D11Ertd400e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 56095
Homologene: 5451
Fbxw18
Name: F-box and WD-40 domain protein 18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 546161
Homologene: 110776
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 672511
Homologene: 45439
4931408C20Rik
Name: RIKEN cDNA 4931408C20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 210940
Homologene: 86827
Farp1
Name: FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived)
Synonyms: Cdep
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 223254
HGNC: HGNC:3591
Homologene: 38098
Arl9
Name: ADP-ribosylation factor-like 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 384185
Homologene: 66207
Cacng5
Name: calcium channel, voltage-dependent, gamma subunit 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 140723
HGNC: HGNC:1409
Homologene: 15424
Phf8-ps
Name: PHD finger protein 8, pseudogene
Synonyms: 4921501E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 74042
VEGA: 17
Homologene: 66275
Olfr1153
Name: olfactory receptor 1153
Synonyms: MOR177-7, GA_x6K02T2Q125-49395950-49396882
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258633
Homologene: 27230
Edn1
Name: endothelin 1
Synonyms: ET-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 13614
VEGA: 13
HGNC: HGNC:3176
Homologene: 1476
Clpx
Name: caseinolytic mitochondrial matrix peptidase chaperone subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 270166
HGNC: HGNC:2088
Homologene: 4851
Herc6
Name: hect domain and RLD 6
Synonyms: CEB1, 4930427L17Rik, Herc5, 1700121D12Rik, 2510038N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 67138
Homologene: 70768
Doxl2
Name: diamine oxidase-like protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 243376
Homologene: 19443
Ro60
Name: Ro60, Y RNA binding protein
Synonyms: SS-A/Ro, A530054J02Rik, Ssa, Trove2, 1810007I17Rik, Ssa2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20822
Homologene: 3383
Vmn1r43
Name: vomeronasal 1 receptor 43
Synonyms: V1ra5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 113847
Homologene: 130651
Ssx2ip
Name: synovial sarcoma, X 2 interacting protein
Synonyms: Adip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99167
Homologene: 8522
Olfr1434
Name: olfactory receptor 1434
Synonyms: Olfr1433, MOR214-4, GA_x6K02T2RE5P-2610001-2610414, GA_x6K02T2RE5P-2618516-2619454, MOR214-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 258680
Homologene: 45029
Gm438
Name: predicted gene 438
Synonyms: LOC329993
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 329993
Homologene: 135765
Olfr301
Name: olfactory receptor 301
Synonyms: MOR221-4, MOR221-1P, GA_x6K02T2NHDJ-9693313-9692378, Olfr1531-ps1, MOR221-1P, MOR211-8P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 257958
Homologene: 128067
Gpr162
Name: G protein-coupled receptor 162
Synonyms: Grca, A-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 14788
Homologene: 8400
C4a
Name: complement component 4A (Rodgers blood group)
Synonyms: Slp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 625018
Homologene: 36030
Gm17019
Name: predicted gene 17019
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 66773
Homologene: 69402
R3hcc1
Name: R3H domain and coiled-coil containing 1
Synonyms: 1700020M16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 71843
Homologene: 136250
Igkv1-135
Name: immunoglobulin kappa variable 1-135
Synonyms: Igkv1-135
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 243420
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 26,685,819 bp
  • A to T, chromosome 1 at 143,770,771 bp
  • T to C, chromosome 2 at 87,896,409 bp
  • A to G, chromosome 3 at 86,919,840 bp
  • G to A, chromosome 3 at 146,437,311 bp
  • A to G, chromosome 4 at 56,906,293 bp
  • A to C, chromosome 4 at 88,630,040 bp
  • T to C, chromosome 4 at 101,745,537 bp
  • T to C, chromosome 4 at 108,626,359 bp
  • T to C, chromosome 4 at 132,670,205 bp
  • T to A, chromosome 4 at 144,778,024 bp
  • T to C, chromosome 4 at 147,675,430 bp
  • A to T, chromosome 5 at 15,032,805 bp
  • T to C, chromosome 5 at 77,006,593 bp
  • C to A, chromosome 5 at 129,571,098 bp
  • C to A, chromosome 6 at 48,975,794 bp
  • A to T, chromosome 6 at 57,647,120 bp
  • C to T, chromosome 6 at 67,610,340 bp
  • T to C, chromosome 6 at 89,869,746 bp
  • T to C, chromosome 6 at 124,860,913 bp
  • T to A, chromosome 7 at 86,412,856 bp
  • C to T, chromosome 7 at 127,092,826 bp
  • T to A, chromosome 8 at 13,409,305 bp
  • A to T, chromosome 9 at 65,317,448 bp
  • A to G, chromosome 9 at 104,024,673 bp
  • G to T, chromosome 9 at 109,691,521 bp
  • A to G, chromosome 10 at 110,759,945 bp
  • T to G, chromosome 11 at 61,808,144 bp
  • T to C, chromosome 11 at 106,255,588 bp
  • T to C, chromosome 11 at 107,877,422 bp
  • C to A, chromosome 11 at 119,433,499 bp
  • T to C, chromosome 13 at 42,301,960 bp
  • C to T, chromosome 13 at 104,187,527 bp
  • T to A, chromosome 14 at 69,698,608 bp
  • C to T, chromosome 14 at 121,235,077 bp
  • T to G, chromosome 15 at 89,167,543 bp
  • A to G, chromosome 16 at 55,915,296 bp
  • G to A, chromosome 17 at 25,205,610 bp
  • A to G, chromosome 17 at 33,065,393 bp
  • T to A, chromosome 17 at 34,813,545 bp
  • C to A, chromosome 19 at 12,283,106 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5520 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043079-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.