Strain Name:
C57BL/6J-MtgxR5522Btlr/Mmmh
Stock Number:
043081-MU
Citation ID:
RRID:MMRRC_043081-MU
Other Names:
R5522 (G1), C57BL/6J-MtgxR5522Btlr
Major Collection:

Gene Information

Epha2
Name: Eph receptor A2
Synonyms: Sek-2, Eck, Sek2, Myk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13836
HGNC: HGNC:3386
Homologene: 20929
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, DPEAAE, Cspg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77595
Homologene: 28122
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: CD221, IGF-1R, line 186, hyft, A330103N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Taok3
Name: TAO kinase 3
Synonyms: A430105I05Rik, 2900006A08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330177
Homologene: 83279
Tmem104
Name: transmembrane protein 104
Synonyms: C630005D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 320534
Homologene: 9802
Taf3
Name: TATA-box binding protein associated factor 3
Synonyms: mTAFII140, 4933439M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 209361
Homologene: 35415
Snrnp70
Name: small nuclear ribonucleoprotein 70 (U1)
Synonyms: Rnulp70, U1-70, 2700022N21Rik, 3200002N22Rik, Srnp70, Snrp70
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20637
Homologene: 20672
Tango6
Name: transport and golgi organization 6
Synonyms: Tmco7, Tango6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 272538
Homologene: 52121
Grb10
Name: growth factor receptor bound protein 10
Synonyms: Meg1, maternally expressed gene 1, 5730571D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14783
HGNC: HGNC:4564
Homologene: 3882
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Usp24
Name: ubiquitin specific peptidase 24
Synonyms: 2810030C21Rik, 2700066K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329908
Homologene: 35420
Nuf2
Name: NUF2, NDC80 kinetochore complex component
Synonyms: 2410003C07Rik, Nuf2R, Cdca1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66977
Homologene: 40205
Rars
Name: arginyl-tRNA synthetase
Synonyms: 2610037E21Rik, 2610011N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 104458
HGNC: HGNC:9870
Homologene: 68281
Adgrl1
Name: adhesion G protein-coupled receptor L1
Synonyms: Lec2, lectomedin-2, 2900070I05Rik, Lphn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330814
Homologene: 8951
Unc5b
Name: unc-5 netrin receptor B
Synonyms: 6330415E02Rik, Unc5h2, D10Bwg0792e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 107449
VEGA: 10
Homologene: 32538
Scamp3
Name: secretory carrier membrane protein 3
Synonyms: Sc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 24045
Homologene: 4164
Zranb1
Name: zinc finger, RAN-binding domain containing 1
Synonyms: D7Wsu87e, 9330160G10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 360216
Homologene: 9728
Ipmk
Name: inositol polyphosphate multikinase
Synonyms: 2410017C19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 69718
Homologene: 76214
Cyp4x1
Name: cytochrome P450, family 4, subfamily x, polypeptide 1
Synonyms: Cyp4a28-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 81906
Homologene: 75853
Pbrm1
Name: polybromo 1
Synonyms: BAF180, 2610016F04Rik, 2310032M22Rik, Pb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66923
Homologene: 10044
Zcchc2
Name: zinc finger, CCHC domain containing 2
Synonyms: 9930114B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227449
Homologene: 9808
Tmem231
Name: transmembrane protein 231
Synonyms: 4932417I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234740
Homologene: 78035
Exph5
Name: exophilin 5
Synonyms: slac2-b, B130009M24Rik, AC079869.22gm5, Slac2b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320051
Homologene: 9007
Myo3a
Name: myosin IIIA
Synonyms: 9030416P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 667663
HGNC: HGNC:7601
Homologene: 49486
Fyco1
Name: FYVE and coiled-coil domain containing 1
Synonyms: Mem2, 2810409M01Rik, ZFYVE7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17281
Homologene: 11561
Sctr
Name: secretin receptor
Synonyms: 6530402O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 319229
Homologene: 68290
Nfatc1
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1
Synonyms: NFAT2, NFATc, NF-ATc, 2210017P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 18018
VEGA: 18
HGNC: HGNC:7775
Homologene: 32336
Plac8
Name: placenta-specific 8
Synonyms: onzin, C15, D5Wsu111e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231507
Homologene: 32328
Olfr183
Name: olfactory receptor 183
Synonyms: GA_x54KRFPKG5P-55229158-55230087, MOR183-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258478
Homologene: 27178
Xab2
Name: XPA binding protein 2
Synonyms: 0610041O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67439
Homologene: 5738
Vmn1r195
Name: vomeronasal 1 receptor 195
Synonyms: V1ri6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 171257
Homologene: 110880
Cerkl
Name: ceramide kinase-like
Synonyms: Rp26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228094
Homologene: 52396
Ugt2b37
Name: UDP glucuronosyltransferase 2 family, polypeptide B37
Synonyms: 0610033E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 112417
Homologene: 137225
Sh2d4a
Name: SH2 domain containing 4A
Synonyms: SH2A, 2210402M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72281
Homologene: 11117
Csmd2
Name: CUB and Sushi multiple domains 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329942
Homologene: 89034
Cd69
Name: CD69 antigen
Synonyms: VEA, AIM, 5830438K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12515
HGNC: HGNC:1694
Homologene: 128584
Tgfbr3
Name: transforming growth factor, beta receptor III
Synonyms: betaglycan, TBRIII, 1110036H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21814
Homologene: 2436
Cfap57
Name: cilia and flagella associated protein 57
Synonyms: C130004B06Rik, LOC384050, 1110020C03Rik, Wdr65
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68625
Homologene: 51350
Gemin6
Name: gem nuclear organelle associated protein 6
Synonyms: 2610019B15Rik, 2810470M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 67242
Homologene: 11711
Upf3a
Name: UPF3 regulator of nonsense transcripts homolog A (yeast)
Synonyms: 4930546M19Rik, RENT3A, UPF3, 2600001C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67031
Homologene: 23395
Plbd1
Name: phospholipase B domain containing 1
Synonyms: 1100001H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66857
Homologene: 11745
Mchr1
Name: melanin-concentrating hormone receptor 1
Synonyms: melanin-concentrating hormone receptor 1, MCH-1R, Gpr24-9, Mch1r, Gpr24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 207911
VEGA: 15
HGNC: HGNC:4479
Homologene: 21097
Dlgap1
Name: DLG associated protein 1
Synonyms: SAPAP1, DAP-1 beta, D17Bwg0511e, GKAP/SAPAP, Sapap1, 4933422O14Rik, Gkap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224997
HGNC: HGNC:2905
Homologene: 31258
Ceacam5
Name: carcinoembryonic antigen-related cell adhesion molecule 5
Synonyms: 1600029H12Rik, Psg30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73250
Homologene: 115938
Kti12
Name: KTI12 homolog, chromatin associated
Synonyms: 1110001A12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100087
Homologene: 6347
Olfr401
Name: olfactory receptor 401
Synonyms: GA_x6K02T2P1NL-4278037-4278984, MOR255-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258701
HGNC: HGNC:8282
Homologene: 73922
Pcdhb6
Name: protocadherin beta 6
Synonyms: Pcdhb5B, PcdhbF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93877
Homologene: 62177
Atp13a2
Name: ATPase type 13A2
Synonyms: 1110012E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74772
Homologene: 56940
Krt32
Name: keratin 32
Synonyms: mHa2, Krt1-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16670
HGNC: HGNC:6449
Homologene: 68246
Zfp189
Name: zinc finger protein 189
Synonyms: C430015I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230162
Homologene: 20737
Olfr781
Name: olfactory receptor 781
Synonyms: GA_x6K02T2PULF-11013616-11014551, MOR114-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258723
Homologene: 37000
Tssk3
Name: testis-specific serine kinase 3
Synonyms: Tssk-3, 4930594I21Rik, Stk22d, Stk22c, 1700014N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 58864
Homologene: 62163
Ighv1-66
Name: immunoglobulin heavy variable 1-66
Synonyms: Gm16901
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 380824
Vmn2r40
Name: vomeronasal 2, receptor 40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100042781
Homologene: 113703
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,257,873 bp
  • A to G, chromosome 1 at 106,023,696 bp
  • A to G, chromosome 1 at 120,036,416 bp
  • A to G, chromosome 1 at 169,498,884 bp
  • T to C, chromosome 2 at 9,941,005 bp
  • T to A, chromosome 2 at 22,574,341 bp
  • T to C, chromosome 2 at 79,392,984 bp
  • T to C, chromosome 3 at 89,177,622 bp
  • T to C, chromosome 3 at 90,154,665 bp
  • T to C, chromosome 4 at 32,685,783 bp
  • C to T, chromosome 4 at 49,529,739 bp
  • T to A, chromosome 4 at 106,372,721 bp
  • T to A, chromosome 4 at 108,848,423 bp
  • C to A, chromosome 4 at 115,121,977 bp
  • A to T, chromosome 4 at 118,595,888 bp
  • A to T, chromosome 4 at 128,059,134 bp
  • G to A, chromosome 4 at 129,489,550 bp
  • T to A, chromosome 4 at 141,004,360 bp
  • T to A, chromosome 4 at 141,308,556 bp
  • T to C, chromosome 5 at 87,240,900 bp
  • T to A, chromosome 5 at 100,562,718 bp
  • A to G, chromosome 5 at 107,142,478 bp
  • A to G, chromosome 5 at 117,273,757 bp
  • A to T, chromosome 6 at 129,271,416 bp
  • A to T, chromosome 6 at 136,617,300 bp
  • T to A, chromosome 7 at 8,908,204 bp
  • A to T, chromosome 7 at 17,715,080 bp
  • C to T, chromosome 7 at 45,377,177 bp
  • C to A, chromosome 7 at 68,183,510 bp
  • T to C, chromosome 7 at 132,983,949 bp
  • A to T, chromosome 8 at 3,611,718 bp
  • T to A, chromosome 8 at 13,795,497 bp
  • T to C, chromosome 8 at 68,296,697 bp
  • T to C, chromosome 8 at 83,923,075 bp
  • T to C, chromosome 8 at 106,695,598 bp
  • T to A, chromosome 8 at 111,918,410 bp
  • T to C, chromosome 9 at 53,374,313 bp
  • G to A, chromosome 9 at 123,794,771 bp
  • T to C, chromosome 10 at 60,778,195 bp
  • C to A, chromosome 10 at 71,363,474 bp
  • A to T, chromosome 10 at 129,332,929 bp
  • T to C, chromosome 11 at 11,936,746 bp
  • A to T, chromosome 11 at 35,817,368 bp
  • A to G, chromosome 11 at 74,121,658 bp
  • A to T, chromosome 11 at 100,086,671 bp
  • G to A, chromosome 11 at 115,188,323 bp
  • T to A, chromosome 12 at 115,593,135 bp
  • A to G, chromosome 13 at 22,278,950 bp
  • T to C, chromosome 13 at 89,691,810 bp
  • A to G, chromosome 14 at 31,089,563 bp
  • A to T, chromosome 15 at 81,238,010 bp
  • A to T, chromosome 16 at 58,999,905 bp
  • C to T, chromosome 16 at 81,434,878 bp
  • T to C, chromosome 17 at 70,516,998 bp
  • T to G, chromosome 17 at 80,227,749 bp
  • A to G, chromosome 18 at 37,334,349 bp
  • T to C, chromosome 18 at 80,653,529 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5522 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043081-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.