Strain Name:
C57BL/6J-MtgxR5536Btlr/Mmmh
Stock Number:
043094-MU
Citation ID:
RRID:MMRRC_043094-MU
Other Names:
R5536 (G1), C57BL/6J-MtgxR5536Btlr
Major Collection:

Gene Information

Mthfr
Name: methylenetetrahydrofolate reductase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17769
HGNC: HGNC:7436
Homologene: 4349
Taf8
Name: TATA-box binding protein associated factor 8
Synonyms: Taf8, Tbn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 63856
VEGA: 17
Homologene: 11094
Lpp
Name: LIM domain containing preferred translocation partner in lipoma
Synonyms: B130055L10Rik, 9430020K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 210126
VEGA: 16
HGNC: HGNC:6679
Homologene: 4075
Plekha1
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Synonyms: TAPP1, C920009D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101476
Homologene: 11001
Srgap2
Name: SLIT-ROBO Rho GTPase activating protein 2
Synonyms: FBP2, 9930124L22Rik, Fnbp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14270
Homologene: 52683
Kdm3a
Name: lysine (K)-specific demethylase 3A
Synonyms: 1700105C21Rik, Tsga, C230043E16Rik, Jmjd1, Jmjd1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 104263
Homologene: 10196
Psmb2
Name: proteasome (prosome, macropain) subunit, beta type 2
Synonyms: HC7-I, D4Wsu33e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26445
HGNC: HGNC:9539
Homologene: 2088
Atp2a2
Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Synonyms: sarco/endoplasmic reticulum Ca2+-ATPase 2, SERCA2, 9530097L16Rik, D5Wsu150e, SERCA2B, Serca2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11938
HGNC: HGNC:812
Homologene: 80167
Tle1
Name: transducin-like enhancer of split 1
Synonyms: Grg1, Estm14, C230057C06Rik, Tle4l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21885
Homologene: 21058
Nol10
Name: nucleolar protein 10
Synonyms: LOC217431
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217431
VEGA: 12
Homologene: 5998
Tmem87a
Name: transmembrane protein 87A
Synonyms: A930025J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 211499
Homologene: 9165
Rad17
Name: RAD17 checkpoint clamp loader component
Synonyms: MmRad24, 9430035O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19356
VEGA: 13
HGNC: HGNC:9807
Homologene: 32117
Adrm1
Name: adhesion regulating molecule 1
Synonyms: ARM-1, 2510006J17Rik, Gp110, 1110063P18Rik, Rpn13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56436
Homologene: 10513
Zbtb40
Name: zinc finger and BTB domain containing 40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230848
Homologene: 8912
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: LOC381341, Dnahc7, Dnahc7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 627872
Homologene: 41287
Lama5
Name: laminin, alpha 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16776
HGNC: HGNC:6485
Homologene: 4060
Rhot1
Name: ras homolog family member T1
Synonyms: Miro1, 2210403N23Rik, FLJ11040, Arht1, C430039G08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 59040
Homologene: 56803
Cdan1
Name: congenital dyserythropoietic anemia, type I (human)
Synonyms: CDA-I, CDA1, 1500015A01Rik, codanin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68968
HGNC: HGNC:1713
Homologene: 16324
Zkscan8
Name: zinc finger with KRAB and SCAN domains 8
Synonyms: 2510038J07Rik, LD5-1, D430019P06Rik, Zfp192
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 93681
Homologene: 21268
Appl1
Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Synonyms: 7330406P05Rik, 2900057D21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 72993
Homologene: 32143
Raph1
Name: Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Synonyms: C730009O10Rik, 9430025M21Rik, lamellipodin, Lpd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77300
Homologene: 71030
Nup153
Name: nucleoporin 153
Synonyms: B130015D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218210
HGNC: HGNC:8062
Homologene: 68442
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Usf3
Name: upstream transcription factor family member 3
Synonyms: LOC207806, LOC385650, 5530400K22Rik, Gm608
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 207806
Homologene: 19504
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545370
Homologene: 23741
Marco
Name: macrophage receptor with collagenous structure
Synonyms: Scara2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17167
HGNC: HGNC:6895
Homologene: 4928
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241516
Homologene: 110349
1110002E22Rik
Name: RIKEN cDNA 1110002E22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 102634333
Homologene: 131709
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Gucy2g
Name: guanylate cyclase 2g
Synonyms: 2410077I05Rik, GC-G
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 73707
Homologene: 44544
Gpr179
Name: G protein-coupled receptor 179
Synonyms: 5330439C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217143
Homologene: 34917
Kmt2d
Name: lysine (K)-specific methyltransferase 2D
Synonyms: C430014K11Rik, Mll4, Mll2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 381022
HGNC: HGNC:7133
Homologene: 86893
Acap1
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Synonyms: Centb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216859
Homologene: 22835
AW551984
Name: expressed sequence AW551984
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244810
Zfp932
Name: zinc finger protein 932
Synonyms: 2310001H12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69504
Homologene: 137770
Zmat4
Name: zinc finger, matrin type 4
Synonyms: 9630048M01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320158
Homologene: 81899
Vmn2r54
Name: vomeronasal 2, receptor 54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 666085
Homologene: 104040
Tmtc4
Name: transmembrane and tetratricopeptide repeat containing 4
Synonyms: 5730419O14Rik, 4930403J22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 70551
Homologene: 32796
Aadac
Name: arylacetamide deacetylase
Synonyms: 5033417E09Rik, Aada
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67758
HGNC: HGNC:17
Homologene: 37436
Ido2
Name: indoleamine 2,3-dioxygenase 2
Synonyms: C230043N17Rik, Ido2, Indol1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 209176
Homologene: 48830
Nlrx1
Name: NLR family member X1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270151
VEGA: 9
Homologene: 11623
E2f6
Name: E2F transcription factor 6
Synonyms: EMA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 50496
VEGA: 12
HGNC: HGNC:3120
Homologene: 1473
Tprn
Name: taperin
Synonyms: C430004E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 97031
Homologene: 52156
Olfr1269
Name: olfactory receptor 1269
Synonyms: GA_x6K02T2Q125-51551618-51550689, MOR228-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258339
Homologene: 133727
Col23a1
Name: collagen, type XXIII, alpha 1
Synonyms: 2810458L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237759
Homologene: 72101
Lpo
Name: lactoperoxidase
Synonyms: 5830499B15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76113
HGNC: HGNC:6678
Homologene: 21240
Slc5a6
Name: solute carrier family 5 (sodium-dependent vitamin transporter), member 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330064
Homologene: 23277
Gm3095
Name: predicted gene 3095
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100041012
Homologene: 115686
Zfp551
Name: zinc fingr protein 551
Synonyms: 9630004E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 619331
Homologene: 64631
Pcdha7
Name: protocadherin alpha 7
Synonyms: Cnr4, Crnr4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12939
HGNC: HGNC:8673
Homologene: 117962
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 53,425,253 bp
  • C to A, chromosome 1 at 60,501,727 bp
  • T to C, chromosome 1 at 120,504,735 bp
  • T to A, chromosome 1 at 131,300,390 bp
  • T to A, chromosome 1 at 150,755,291 bp
  • G to A, chromosome 2 at 25,263,357 bp
  • A to T, chromosome 2 at 82,987,059 bp
  • A to G, chromosome 2 at 90,118,839 bp
  • C to A, chromosome 2 at 120,397,430 bp
  • A to G, chromosome 2 at 120,745,132 bp
  • A to G, chromosome 2 at 180,172,188 bp
  • C to T, chromosome 2 at 180,189,349 bp
  • T to G, chromosome 3 at 60,039,563 bp
  • G to T, chromosome 3 at 138,066,388 bp
  • G to A, chromosome 4 at 72,145,343 bp
  • T to A, chromosome 4 at 126,684,209 bp
  • G to T, chromosome 4 at 136,987,331 bp
  • T to C, chromosome 4 at 148,044,483 bp
  • A to G, chromosome 5 at 31,043,102 bp
  • T to A, chromosome 5 at 110,009,847 bp
  • T to C, chromosome 5 at 122,457,182 bp
  • A to C, chromosome 6 at 71,611,936 bp
  • A to T, chromosome 7 at 12,415,561 bp
  • A to C, chromosome 7 at 12,632,416 bp
  • G to T, chromosome 7 at 130,909,601 bp
  • T to C, chromosome 8 at 16,288,660 bp
  • T to C, chromosome 8 at 23,748,492 bp
  • T to C, chromosome 8 at 24,540,657 bp
  • A to G, chromosome 9 at 39,592,873 bp
  • A to T, chromosome 9 at 44,263,886 bp
  • C to A, chromosome 11 at 51,567,949 bp
  • A to G, chromosome 11 at 59,107,871 bp
  • C to T, chromosome 11 at 69,889,307 bp
  • C to A, chromosome 11 at 80,265,787 bp
  • T to C, chromosome 11 at 87,816,563 bp
  • T to A, chromosome 11 at 97,343,815 bp
  • T to G, chromosome 12 at 16,824,684 bp
  • C to A, chromosome 12 at 17,416,137 bp
  • T to C, chromosome 13 at 21,526,668 bp
  • T to C, chromosome 13 at 46,683,009 bp
  • C to A, chromosome 13 at 100,631,104 bp
  • G to T, chromosome 14 at 3,964,502 bp
  • G to C, chromosome 14 at 26,923,780 bp
  • G to A, chromosome 14 at 122,932,879 bp
  • G to A, chromosome 15 at 98,852,109 bp
  • A to G, chromosome 16 at 24,845,206 bp
  • A to G, chromosome 16 at 44,217,370 bp
  • C to A, chromosome 17 at 47,494,482 bp
  • G to A, chromosome 18 at 36,975,250 bp
  • T to A, chromosome 19 at 55,237,927 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5536 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043094-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.