Strain Name:
C57BL/6J-MtgxR5657Btlr/Mmmh
Stock Number:
043171-MU
Citation ID:
RRID:MMRRC_043171-MU
Other Names:
R5657 (G1), C57BL/6J-MtgxR5657Btlr
Major Collection:

Gene Information

Epha2
Name: Eph receptor A2
Synonyms: Sek-2, Eck, Sek2, Myk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13836
HGNC: HGNC:3386
Homologene: 20929
Foxm1
Name: forkhead box M1
Synonyms: Mpm2, WIN, HFH-11B, Trident, Fkh16, Foxm1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14235
HGNC: HGNC:3818
Homologene: 7318
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66860
Homologene: 18946
Ptpra
Name: protein tyrosine phosphatase, receptor type, A
Synonyms: RPTRalpha, PTPalpha, PTP[a], Ptpa, RPTPalpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19262
HGNC: HGNC:9664
Homologene: 20621
Mapkapk3
Name: mitogen-activated protein kinase-activated protein kinase 3
Synonyms: MK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102626
HGNC: HGNC:6888
Homologene: 55836
Prpf38a
Name: PRP38 pre-mRNA processing factor 38 (yeast) domain containing A
Synonyms: 2410002M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230596
Homologene: 13146
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Bloc1s6
Name: biogenesis of lysosomal organelles complex-1, subunit 6, pallidin
Synonyms: BLOC-1 subunit, BLOC-1, Pldn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18457
HGNC: HGNC:8549
Homologene: 40841
Ticam1
Name: toll-like receptor adaptor molecule 1
Synonyms: Trif, TICAM-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106759
Homologene: 8605
Sun2
Name: Sad1 and UNC84 domain containing 2
Synonyms: B230369L08Rik, Unc84b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223697
Homologene: 9113
Clhc1
Name: clathrin heavy chain linker domain containing 1
Synonyms: 1700034F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 73324
Homologene: 17569
Bach1
Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
Synonyms: 6230421P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12013
HGNC: HGNC:935
Homologene: 916
Sugp2
Name: SURP and G patch domain containing 2
Synonyms: Sfrs14, Srsf14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234373
Homologene: 8923
Tor1aip1
Name: torsin A interacting protein 1
Synonyms: LAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208263
Homologene: 9208
Galnt12
Name: polypeptide N-acetylgalactosaminyltransferase 12
Synonyms: A630062B03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230145
Homologene: 11637
Dnajc13
Name: DnaJ heat shock protein family (Hsp40) member C13
Synonyms: Rme8, LOC382100, D030002L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235567
Homologene: 13574
Trpc6
Name: transient receptor potential cation channel, subfamily C, member 6
Synonyms: mtrp6, Trrp6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22068
VEGA: 9
Homologene: 37944
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545370
Homologene: 23741
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, mKIAA0811, Fath2, EMI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: lrd, b2b1203Clo, b2b1279Clo, b2b598Clo, b2b1289Clo, b2b1727Clo, Dnahc11, avc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Col27a1
Name: collagen, type XXVII, alpha 1
Synonyms: 5730512J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 373864
Homologene: 69400
Vmn2r100
Name: vomeronasal 2, receptor 100
Synonyms: EG627537
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 627537
Homologene: 129750
Naip6
Name: NLR family, apoptosis inhibitory protein 6
Synonyms: Naip-rs4A, Naip-rs4, Birc1f
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17952
HGNC: HGNC:7634
Homologene: 113589
Ahnak
Name: AHNAK nucleoprotein (desmoyokin)
Synonyms: DY6, 2310047C17Rik, 1110004P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Grin2b
Name: glutamate receptor, ionotropic, NMDA2B (epsilon 2)
Synonyms: NMDAR2B, NR2B, Nmdar2b, GluRepsilon2, GluN2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14812
HGNC: HGNC:4586
Homologene: 646
Zfp787
Name: zinc finger protein 787
Synonyms: 2210018M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67109
Homologene: 18983
Col6a4
Name: collagen, type VI, alpha 4
Synonyms: EG235580, 1110001D15Rik, Dvwa, Vwa6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68553
Homologene: 130754
Plekha6
Name: pleckstrin homology domain containing, family A member 6
Synonyms: Pepp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240753
Homologene: 135779
Cracr2a
Name: calcium release activated channel regulator 2A
Synonyms: LOC381812, LOC243645, Efcab4b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 381812
Homologene: 41883
Jade2
Name: jade family PHD finger 2
Synonyms: 1200017K05Rik, Phf15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76901
Homologene: 9082
Clca3b
Name: chloride channel accessory 3B
Synonyms: Clca4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229927
Homologene: 135610
Itih3
Name: inter-alpha trypsin inhibitor, heavy chain 3
Synonyms: Intin3, Itih-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16426
HGNC: HGNC:6168
Homologene: 1669
Rabl2
Name: RAB, member RAS oncogene family-like 2
Synonyms: 1110031N17Rik, Rabl2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 68708
Homologene: 90913
Agbl5
Name: ATP/GTP binding protein-like 5
Synonyms: 9430057O19Rik, Ccp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231093
Homologene: 11053
Olfr393
Name: olfactory receptor 393
Synonyms: GA_x6K02T2P1NL-4004140-4003208, MOR135-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259010
Homologene: 79335
Kif12
Name: kinesin family member 12
Synonyms: N-9 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16552
Homologene: 7796
Dpf3
Name: D4, zinc and double PHD fingers, family 3
Synonyms: cer-d4, 2810403B03Rik, CERD4, Gm18872
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 70127
Homologene: 129842
Plppr2
Name: phospholipid phosphatase related 2
Synonyms: PRG-4, BC018242, Lppr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235044
Homologene: 11229
Cyb561d1
Name: cytochrome b-561 domain containing 1
Synonyms: 1600010M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72023
Homologene: 45651
Slc26a10
Name: solute carrier family 26, member 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216441
VEGA: 10
Homologene: 66953
Reep1
Name: receptor accessory protein 1
Synonyms: D6Ertd253e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 52250
Homologene: 41504
Gm2396
Name: predicted gene 2396
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 105245043
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 133,272,307 bp
  • A to G, chromosome 1 at 150,658,562 bp
  • G to T, chromosome 1 at 156,007,488 bp
  • A to G, chromosome 2 at 59,834,707 bp
  • T to A, chromosome 2 at 122,738,657 bp
  • G to A, chromosome 2 at 130,504,284 bp
  • A to G, chromosome 3 at 108,200,692 bp
  • C to A, chromosome 3 at 144,827,383 bp
  • T to C, chromosome 4 at 47,104,150 bp
  • T to C, chromosome 4 at 63,169,347 bp
  • T to C, chromosome 4 at 63,225,310 bp
  • T to C, chromosome 4 at 108,568,424 bp
  • T to C, chromosome 4 at 141,323,494 bp
  • A to G, chromosome 5 at 30,894,046 bp
  • A to G, chromosome 6 at 71,761,374 bp
  • G to A, chromosome 6 at 127,604,007 bp
  • A to T, chromosome 6 at 128,373,388 bp
  • T to A, chromosome 6 at 135,733,087 bp
  • T to C, chromosome 7 at 6,133,054 bp
  • GC to GCGGCGGCGTC, chromosome 7 at 97,579,934 bp
  • T to A, chromosome 8 at 70,248,416 bp
  • C to T, chromosome 9 at 8,609,807 bp
  • T to C, chromosome 9 at 21,947,615 bp
  • A to T, chromosome 9 at 88,921,089 bp
  • A to G, chromosome 9 at 104,228,537 bp
  • A to G, chromosome 9 at 106,072,198 bp
  • T to C, chromosome 9 at 107,284,904 bp
  • T to C, chromosome 10 at 127,174,964 bp
  • A to G, chromosome 11 at 29,561,431 bp
  • A to G, chromosome 11 at 51,816,987 bp
  • G to T, chromosome 11 at 55,310,681 bp
  • T to C, chromosome 11 at 73,847,540 bp
  • T to C, chromosome 12 at 83,325,011 bp
  • T to A, chromosome 12 at 117,883,617 bp
  • C to A, chromosome 13 at 100,300,401 bp
  • T to C, chromosome 14 at 30,921,418 bp
  • C to A, chromosome 15 at 79,727,949 bp
  • T to C, chromosome 15 at 89,588,213 bp
  • A to G, chromosome 16 at 87,719,285 bp
  • T to A, chromosome 17 at 19,504,916 bp
  • TCACACA to TCACA, chromosome 17 at 56,270,629 bp
  • T to C, chromosome 19 at 9,014,615 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5657 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043171-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.