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Strain Name:
C57BL/6J-MtgxR5605Btlr/Mmmh
Stock Number:
043270-MU
Citation ID:
RRID:MMRRC_043270-MU
Other Names:
R5605 (G1)
Major Collection:

Strain Information

D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Ylpm1
Name: YLP motif containing 1
Synonyms: ZAP, Zap3, A930013E17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56531
Homologene: 87707
Stk4
Name: serine/threonine kinase 4
Synonyms: Ysk3, Kas-2, Mst1, sterile 20-like kinase 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58231
Homologene: 55965
Nfyc
Name: nuclear transcription factor-Y gamma
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18046
HGNC: HGNC:7806
Homologene: 7440
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 46,798,235 bp
  • A to T, chromosome 1 at 54,415,926 bp
  • A to T, chromosome 1 at 140,574,743 bp
  • A to G, chromosome 1 at 172,081,150 bp
  • G to A, chromosome 2 at 25,408,945 bp
  • C to T, chromosome 2 at 69,523,299 bp
  • G to A, chromosome 2 at 121,397,052 bp
  • T to C, chromosome 2 at 128,738,307 bp
  • T to C, chromosome 2 at 143,749,245 bp
  • T to C, chromosome 2 at 164,079,566 bp
  • A to G, chromosome 3 at 58,625,119 bp
  • G to A, chromosome 3 at 90,268,368 bp
  • T to C, chromosome 3 at 129,917,009 bp
  • T to C, chromosome 3 at 142,501,276 bp
  • A to G, chromosome 4 at 9,281,751 bp
  • T to C, chromosome 4 at 32,765,664 bp
  • A to G, chromosome 4 at 45,018,723 bp
  • A to G, chromosome 4 at 120,790,489 bp
  • A to T, chromosome 5 at 62,615,067 bp
  • T to C, chromosome 5 at 115,283,717 bp
  • T to C, chromosome 6 at 24,505,621 bp
  • A to T, chromosome 6 at 38,191,764 bp
  • C to T, chromosome 6 at 58,857,727 bp
  • C to T, chromosome 7 at 6,480,326 bp
  • T to C, chromosome 7 at 17,747,236 bp
  • T to C, chromosome 7 at 98,006,300 bp
  • T to C, chromosome 7 at 104,840,640 bp
  • C to T, chromosome 7 at 110,946,002 bp
  • C to T, chromosome 7 at 127,215,426 bp
  • T to C, chromosome 8 at 126,949,419 bp
  • T to C, chromosome 9 at 67,049,035 bp
  • A to G, chromosome 9 at 77,045,412 bp
  • A to G, chromosome 10 at 9,769,746 bp
  • C to T, chromosome 10 at 34,403,695 bp
  • C to A, chromosome 10 at 117,045,900 bp
  • A to T, chromosome 11 at 70,944,070 bp
  • T to C, chromosome 11 at 75,771,960 bp
  • A to T, chromosome 12 at 16,708,726 bp
  • G to A, chromosome 12 at 85,028,853 bp
  • T to A, chromosome 12 at 88,011,998 bp
  • T to A, chromosome 12 at 103,199,904 bp
  • T to C, chromosome 12 at 104,241,040 bp
  • T to A, chromosome 14 at 31,035,992 bp
  • A to T, chromosome 15 at 9,609,520 bp
  • A to T, chromosome 15 at 12,592,350 bp
  • C to T, chromosome 15 at 100,697,225 bp
  • G to T, chromosome 15 at 102,503,865 bp
  • A to G, chromosome 17 at 18,136,501 bp
  • T to C, chromosome 17 at 24,700,759 bp
  • T to C, chromosome 17 at 34,309,833 bp
  • C to A, chromosome 17 at 47,623,144 bp
  • T to G, chromosome 17 at 50,047,407 bp
  • C to T, chromosome 18 at 20,399,539 bp
  • T to C, chromosome 18 at 65,174,244 bp
  • C to A, chromosome 18 at 67,442,355 bp
  • A to T, chromosome 19 at 10,658,038 bp
  • T to A, chromosome 19 at 41,582,863 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5605 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043270-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.