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Strain Name:
C57BL/6J-MtgxR5729Btlr/Mmmh
Stock Number:
043346-MU
Citation ID:
RRID:MMRRC_043346-MU
Other Names:
R5729 (G1)
Major Collection:

Strain Information

Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Pitpnc1
Name: phosphatidylinositol transfer protein, cytoplasmic 1
Synonyms: C330017I21Rik, 5830436L09Rik, 1110020B03Rik, RDGB-BETA, RDGBB1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71795
Homologene: 138301
Pigv
Name: phosphatidylinositol glycan anchor biosynthesis, class V
Synonyms: D430024F16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230801
Homologene: 5354
Dnajc21
Name: DnaJ heat shock protein family (Hsp40) member C21
Synonyms: 9930116P15Rik, 4930461P20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78244
Homologene: 6752
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Mat2b
Name: methionine adenosyltransferase 2 non-catalytic beta subunit methionine
Synonyms: MATIIbeta, 2410018D16Rik, 1110064C04Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 108645
HGNC: HGNC:6905
Homologene: 8323
Spop
Name: speckle-type BTB/POZ protein
Synonyms: TEF2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20747
Homologene: 68354
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to G, chromosome 1 at 85,589,118 bp
  • C to T, chromosome 1 at 87,936,332 bp
  • A to G, chromosome 1 at 172,293,371 bp
  • A to T, chromosome 1 at 177,796,258 bp
  • T to C, chromosome 2 at 4,763,274 bp
  • C to T, chromosome 2 at 18,715,714 bp
  • A to T, chromosome 2 at 65,317,021 bp
  • A to G, chromosome 2 at 66,741,957 bp
  • A to G, chromosome 2 at 70,105,739 bp
  • G to A, chromosome 2 at 174,093,499 bp
  • A to G, chromosome 4 at 3,870,971 bp
  • C to A, chromosome 4 at 117,268,472 bp
  • A to G, chromosome 4 at 123,924,852 bp
  • T to A, chromosome 4 at 126,830,415 bp
  • A to T, chromosome 4 at 133,664,823 bp
  • T to A, chromosome 5 at 15,935,039 bp
  • A to T, chromosome 5 at 65,277,452 bp
  • T to C, chromosome 5 at 136,093,162 bp
  • T to A, chromosome 5 at 138,290,223 bp
  • T to C, chromosome 6 at 14,719,763 bp
  • T to G, chromosome 6 at 58,939,307 bp
  • A to G, chromosome 6 at 115,965,877 bp
  • T to C, chromosome 6 at 127,607,236 bp
  • T to C, chromosome 7 at 12,600,806 bp
  • A to T, chromosome 7 at 17,210,534 bp
  • G to T, chromosome 7 at 19,005,247 bp
  • A to G, chromosome 7 at 30,712,426 bp
  • A to G, chromosome 7 at 45,220,742 bp
  • A to T, chromosome 7 at 79,600,623 bp
  • A to T, chromosome 7 at 126,764,807 bp
  • G to A, chromosome 7 at 127,885,454 bp
  • A to T, chromosome 7 at 132,558,255 bp
  • T to C, chromosome 8 at 33,268,778 bp
  • G to A, chromosome 8 at 122,454,890 bp
  • A to T, chromosome 9 at 44,933,329 bp
  • G to C, chromosome 9 at 90,207,872 bp
  • A to T, chromosome 11 at 40,682,546 bp
  • A to G, chromosome 11 at 41,967,623 bp
  • G to A, chromosome 11 at 52,118,029 bp
  • A to G, chromosome 11 at 95,485,849 bp
  • A to T, chromosome 11 at 102,883,078 bp
  • G to T, chromosome 11 at 107,337,438 bp
  • G to T, chromosome 11 at 114,864,267 bp
  • T to C, chromosome 12 at 55,565,905 bp
  • A to G, chromosome 14 at 52,160,160 bp
  • A to T, chromosome 14 at 57,722,735 bp
  • A to G, chromosome 14 at 79,355,780 bp
  • A to T, chromosome 15 at 10,449,596 bp
  • G to T, chromosome 15 at 98,066,531 bp
  • T to G, chromosome 15 at 101,476,548 bp
  • T to C, chromosome 17 at 45,426,569 bp
  • A to T, chromosome 17 at 87,078,516 bp
  • A to T, chromosome 18 at 36,667,443 bp
  • A to T, chromosome 18 at 38,202,946 bp
  • C to T, chromosome 18 at 47,281,343 bp
  • A to G, chromosome 18 at 76,858,883 bp
  • A to G, chromosome 19 at 4,802,549 bp
  • A to T, chromosome 19 at 12,035,908 bp
  • C to G, chromosome X at 9,450,750 bp
  • A to T, chromosome Y at 1,381,339 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5729 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043346-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.