Strain Name:
STOCK Ptf1atm1.1(cre)Cvw/Mmnc
Stock Number:
000435-UNC
Citation ID:
RRID:MMRRC_000435-UNC
Other Names:
PTF1-p48

Gene Information

Allele Symbol: Ptf1atm1.1(cre)Cvw (Mus musculus (mouse))
Name: pancreas specific transcription factor, 1a; targeted mutation 1.1, Christopher VE Wright
Synonyms: P48, P48-Cre, Ptf1a, Ptf1a-cre, Ptf1a
Chromosome: 2
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:2387812
Transgene: cre (Enterobacteria phage P1 (bacteriophage P1))
Name: cre recombinase
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations):
Gene Symbol: Ptf1a (Mus musculus (mouse))
Name: pancreas specific transcription factor, 1a
Synonyms: PTF1-p48, bHLHa29
Chromosome: 2
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:1328312
Genetic Alterations
Protein coding regions of Cre and Ptf1a were exchanged at translational initiation codons.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
    Phenotype
    Homozygous: altered pancreatic development with loss of exocrine acini.
    Mammalian Phenotype Terms
    Allelic Composition: Ptf1atm1.1(cre)Cvw/Ptf1atm1.1(cre)Cvw (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

    MeSH Terms
    • Animals
    • Animals, Genetically Modified
    • Cell Differentiation
    • Cell Lineage
    • Duodenum/cytology
    • Duodenum/embryology
    • Gene Expression Regulation
    • Homeodomain Proteins
    • Mice
    • Pancreas/cytology
    • Pancreas/embryology
    • Trans-Activators/metabolism
    • Transcription Factors/physiology
    Strain Development
    The floxed neo cassette was excised from mice carrying Ptf1atm1(cre)Cvw by in vivo cre mediated recombination. The previously inserted cre recombinase remained in place of the entire endogenous coding region.
    • Diabetes/Obesity
    • Endocrine Deficiency
    Donor
    Chris V. Wright, Ph.D., Vanderbilt University
    Primary Reference
    Kawaguchi Y, Cooper B, Gannon M, Ray M, MacDonald RJ, Wright CV. The role of the transcriptional regulator Ptf1a in converting intestinal to pancreatic progenitors. Nat Genet. 2002 Sep;32(1):128-34. Epub 2002 Aug 19. (Medline PMID: 12185368)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
    MMRRC Breeding System
    Backcross
    Breeding Scheme(s)
    • Line is maintained through backcross to B6D2
    Generation
    Unknown
    Overall Breeding Performance
    Unknown
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Unknown Unknown homozygotes are not viable according to donating investigator
    Homozygotes are fertile: Unknown Unknown
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Average litter size
    8
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    000435-UNC-SPERM Cryo-preserved spermatozoa $548.00 / $910.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity2
    000435-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / $4,696.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.