Strain Name:
B6J.B6N-Tmem35atm1(KOMP)Vlcg/TranMmnc
Stock Number:
043504-UNC
Citation ID:
RRID:MMRRC_043504-UNC
Other Names:
C57B6, B6

Gene Information

Allele Symbol: Tmem35atm1(KOMP)Vlcg (Mus musculus (mouse))
Name: transmembrane protein 35A; targeted mutation 1, Velocigene
Chromosome: X
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:4399235
Gene Symbol: Tmem35a (Mus musculus (mouse))
Name: transmembrane protein 35A
Synonyms: 9030603L14Rik, Tmem35
Chromosome: X
Alteration at locus: Knockout
Monarch Initiative (Disease and Phenotype Associations): MGI:1914814
Genetic Alterations
Deletion of Tmem35a (Gene ID:67564) that results in a truncated (non-functional) protein.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    VG11586; Parental Line VGB6
    Phenotype

    Homozygous: Viable, fertile, impaired cognition memory, heightened pain sensitivity.

    Hetero/Hemizygous: Unknown

    Cre-excised Phenotype: Undetermined

    MeSH Terms
      Strain Development

      The mouse knockout (KO) was generated with VG11586 ES cell (targeting the Tmem35 gene) obtained through the University of California (UC) Davis Knock-Out Mouse Project (KOMP) Repository (www.komp.org). Generation of chimeric mice and identification of germ line-targeted founders were performed by the UC Davis Mouse Biology Program. The NeoR cassette was removed from the gene-targeting vector by mating tmem35 heterozygous females (C57BL/6N) to EIIa-Cre transgenic males (FVB, Jackson Stock #003314). The removal of the NeoR cassette was confirmed by the absence of PCR-amplified DNA, using PCR primers specific to the Neo gene. Mice were then backcrossed to C57BL6/J strain by mating tmem35 heterozygous females to wild-type males for 10 generations.

      Suggested Control Mice
      Wild-type littermates
      • Cardiovascular
      • Neurobiology
      • Reproduction
      • Research Tools
      • Sensorineural
      Donor
      Phu Tran, Ph.D., University of Minnesota.

      Colony and Husbandry Informationon

      Colony Surveillance Program and Current Health Reports

      Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
      Coat Color
      Black
      MMRRC Breeding System
      Backcross
      Generation
      >20 generations
      Overall Breeding Performance
      Excellent
      NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
      Viability and Fertility: Female Male Comments
      Homozygotes are viable: Yes Yes
      Homozygotes are fertile: Yes Yes
      Hetero/Hemizygotes are fertile: Yes Yes
      Age Reproductive Decline: Less than 4 months 8 to 12 months
      Bred to Homozygosity
      No
      Average litter size
      5-9
      Recommended wean age
      3 weeks

      Order Request Information

      Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

      Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

      The donor or their institution limits the distribution to non-profit institutions only.

      Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

      Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
      MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
      043504-UNC-EMBRYO Cryo-preserved embryos $1,363.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
      043504-UNC-SPERM Cryo-preserved spermatozoa $548.00 / Non-Profit Aliquot Approximate quantity2
      043504-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

      1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

      2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

      3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

      To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.