Strain Name:
Stock Number:
Citation ID:
Other Names:
Sirt1ER/ER, Sirt1ER

Gene Information

Allele Symbol: Sirt1tm3.1Mcby (Mus musculus (mouse))
Name: sirtuin 1; targeted mutation 3.1,Michael W McBurney
Synonyms: sirt1
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:5903422
Gene Symbol: Sirt1 (Mus musculus (mouse))
Name: sirtuin 1
Synonyms: Sir2alpha, Sir2
Chromosome: 10
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:2135607
Genetic Alterations
We used homologous recombination in the R1 line of embryonic stem (ES) cells to modify the sirt1 gene. A knock-in vector was constructed that replaced all but the 3’ 130 bases of the 1.8 kb 3’UTR of the SIRT1 transcript with a region encoding the hormone-binding domain of the mouse estrogen receptor alpha (ERα) containing a point mutation (G525R) that confers selective binding to 4-hydroxy-tamoxifen (4OHT). This 4OHT-binding domain (referred to hereafter as ER), derived from codons 281 to 599 of the mouse ERα, was fused in frame to the C-terminal end of the SIRT1 coding region. The result is that the level of Sirt1-ER protein and activity can be reversibly modulated.
ES Cell Line

Homozygous: Sirt1ER mice have some mild craniofacial differences (ocular hypotelorism and shortened snout), males are subfertile, and exhibit some lymphocytic infiltration in the salivary and lacrimal glands.

Hetero/Hemizygous: Same as Wild-type.

Cre-excised Phenotype: Undetermined. 

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
    Strain Development
    Sirt1+/ER embryonic stem cells were aggregated with morulae from CD1 mice. After culture to the blastocyst stage, blastocysts were transferred to pseudopregnant CD1 females. Chimeras were identified by coat pigmentation. Male chimeras were bred to 129/sv females. Resulting females carrying the Sirt1ER allele were bred to male mice carrying the cre recombinase under control of the protamine promoter. Resulting males with both the Sirt1ER allele and the prm-cre transgene were bred to 129/sv females. The resulting offspring had deleted pgk-puro that had been inserted downstream of the SirtER allele. Five backcrosses were done into the 129/sv background. Sirt1+/ER mice were intercrossed and resulting homozygous Sirt1ER/ER mice were maintained by brother-sister crosses.
    Suggested Control Mice
    Wild-type littermates
    • Cancer
    • Cell Biology
    • Developmental Biology
    • Diabetes
    • Immunology and Inflammation
    • Metabolism
    • Obesity
    • Research Tools
    Michael McBurney, Ph.D., Ottawa Hospital Research Institute.

    Colony and Husbandry Information

    For more information about this colony's health status contact
    Coat Color
    MMRRC Breeding System
    N5, >F10
    Overall Breeding Performance
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Reduced
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 8 to 12 months 8 to 12 months
    Bred to Homozygosity
    Average litter size
    4-6 pups
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at for more details.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

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    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    Heterozygous / Hemizygous Female
    Heterozygous / Hemizygous Male
    Wild Type Female
    Wild Type Male
    $218.00 / Non-Profit Per Mouse The may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

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