Strain Name:
129-Sirt1tm3.1Mcby/Mmjax
Stock Number:
043520-JAX
Citation ID:
RRID:MMRRC_043520-JAX
Other Names:
Sirt1ER/ER, Sirt1ER

Gene Information

Allele Symbol: Sirt1tm3.1Mcby (Mus musculus (mouse))
Name: sirtuin 1; targeted mutation 3.1,Michael W McBurney
Synonyms: sirt1
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:5903422
Gene Symbol: Sirt1 (Mus musculus (mouse))
Name: sirtuin 1
Synonyms: Sir2alpha, Sir2
Chromosome: 10
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:2135607
Genetic Alterations
We used homologous recombination in the R1 line of embryonic stem (ES) cells to modify the sirt1 gene. A knock-in vector was constructed that replaced all but the 3’ 130 bases of the 1.8 kb 3’UTR of the SIRT1 transcript with a region encoding the hormone-binding domain of the mouse estrogen receptor alpha (ERα) containing a point mutation (G525R) that confers selective binding to 4-hydroxy-tamoxifen (4OHT). This 4OHT-binding domain (referred to hereafter as ER), derived from codons 281 to 599 of the mouse ERα, was fused in frame to the C-terminal end of the SIRT1 coding region. The result is that the level of Sirt1-ER protein and activity can be reversibly modulated.
ES Cell Line
R1
Phenotype

Homozygous: Sirt1ER mice have some mild craniofacial differences (ocular hypotelorism and shortened snout), males are subfertile, and exhibit some lymphocytic infiltration in the salivary and lacrimal glands.

Hetero/Hemizygous: Same as Wild-type.

Cre-excised Phenotype: Undetermined. 

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
    Strain Development
    Sirt1+/ER embryonic stem cells were aggregated with morulae from CD1 mice. After culture to the blastocyst stage, blastocysts were transferred to pseudopregnant CD1 females. Chimeras were identified by coat pigmentation. Male chimeras were bred to 129/sv females. Resulting females carrying the Sirt1ER allele were bred to male mice carrying the cre recombinase under control of the protamine promoter. Resulting males with both the Sirt1ER allele and the prm-cre transgene were bred to 129/sv females. The resulting offspring had deleted pgk-puro that had been inserted downstream of the SirtER allele. Five backcrosses were done into the 129/sv background. Sirt1+/ER mice were intercrossed and resulting homozygous Sirt1ER/ER mice were maintained by brother-sister crosses.
    Suggested Control Mice
    Wild-type littermates
    • Cancer
    • Cell Biology
    • Developmental Biology
    • Diabetes
    • Immunology and Inflammation
    • Metabolism
    • Obesity
    • Research Tools
    Donor
    Michael McBurney, Ph.D., Ottawa Hospital Research Institute.

    Colony and Husbandry Informationon

    For more information about this colony's health status contact csmmrrc@jax.org
    Coat Color
    brown
    MMRRC Breeding System
    Sib-mating
    Generation
    N5, >F10
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Reduced
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 8 to 12 months 8 to 12 months
    Bred to Homozygosity
    No
    Average litter size
    4-6 pups
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    043520-JAX-HET-F
    043520-JAX-HET-M
    043520-JAX-WT-F
    043520-JAX-WT-M
    Heterozygous / Hemizygous Female
    Heterozygous / Hemizygous Male
    Wild Type Female
    Wild Type Male
    $218.00 / Non-Profit Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.