Strain Name:
B6.129S6-Ugcgtm4Rlp/Mmucd
Stock Number:
043532-UCD
Citation ID:
RRID:MMRRC_043532-UCD
Other Names:
floxed Ugcg, glucosylceramide synthase;Glct

Gene Information

Ugcgtm4Rlp
Name: UDP-glucose ceramide glucosyltransferase; targeted mutation 4, Richard L Proia
Synonyms: UgcgloxP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Conditional
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: GlcT-1, Epcs21, Ugcgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Conditional
NCBI: 22234
Homologene: 37763
Genetic Alterations
A loxP site was inserted into intron 6 and loxP sites surrounding the neocassette were placed into the 3' UTR.
Genotype Determination
ES Cell Line
TC1/TC-1 derived from 129S6/SvEvTac
Phenotype

Homozygous: None

Hetero/Hemizygous: None

Cre-excised Phenotype: Undetermined 

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

Strain Development
A genomic clone containing the Ugcg locus was isolated from a 129-mouse library (Genome Systems, St. Louis,MO) using a mouse Ugcg cDNA probe. An 8.5-kb BglII fragment containing exons 6, 7, 8, 9, and the 3' untranslated region was used to construct the pUgcgloxPneo targeting vector using the ploxPneo plasmid as a cloning vehicle. A loxP site was inserted into intron 6 and loxP sites surrounding the neocassette were placed into the 3'untranslated region. The pUgcgloxPneo vector was linearized and electroporated into TC-1 ES cells to target the Ugcg allele. Clones resistant to ganciclovir and G418 were screened by Southern blot analysis with a probe corresponding to exon 5 of Ugcg. The targeting procedure gave about a 3% targeting frequency. One of the targeted clones was used to establish chimeric males,which were bred to C57BL/6 females to obtain germline transmission.

The lab confirmed use of C57BL/6J for embryo generation. The MMRRC will continue to maintain the line using C57BL/6J at the repository (either as egg donors for IVF or to be used, if breeding).
Suggested Control Mice
Wild-type littermates
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes
  • Hematology
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Reproduction
  • Research Tools
  • Sensorineural
  • Virology
Donor
Richard Proia, Ph.D., National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
conventional
Generation
7
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months Greater than 12 months
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043532-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
043532-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
043532-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.