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Strain Name:
C57BL/6J-MtgxR5846Btlr
Stock Number:
044064-MU
Citation ID:
RRID:MMRRC_044064-MU
Other Names:
R5846 (G1)
Major Collection:

Strain Information

Tk1
Name: thymidine kinase 1
Synonyms: Tk-1, D530002A18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21877
Homologene: 37749
Pi4k2a
Name: phosphatidylinositol 4-kinase type 2 alpha
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 84095
VEGA: 19
Homologene: 101681
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: WWP3, Gukmi1, AIP3, BAP1, Baiap1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69697
Homologene: 18966
BC004004
Name: cDNA sequence BC004004
Synonyms: 2400006G15Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 80748
Homologene: 12728
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 34,195,861 bp
  • T to C, chromosome 2 at 10,097,903 bp
  • T to A, chromosome 2 at 67,509,243 bp
  • T to C, chromosome 2 at 68,732,033 bp
  • T to A, chromosome 2 at 76,903,468 bp
  • T to C, chromosome 2 at 150,608,421 bp
  • T to C, chromosome 2 at 181,152,983 bp
  • C to T, chromosome 3 at 88,581,072 bp
  • A to G, chromosome 3 at 108,881,391 bp
  • A to T, chromosome 4 at 56,903,241 bp
  • T to C, chromosome 4 at 140,995,596 bp
  • T to C, chromosome 5 at 62,649,773 bp
  • C to A, chromosome 5 at 107,140,655 bp
  • T to A, chromosome 5 at 124,823,373 bp
  • C to T, chromosome 5 at 137,394,376 bp
  • C to G, chromosome 5 at 137,633,657 bp
  • T to C, chromosome 5 at 142,114,393 bp
  • T to C, chromosome 5 at 144,879,380 bp
  • A to G, chromosome 6 at 3,376,754 bp
  • C to T, chromosome 6 at 35,512,290 bp
  • A to T, chromosome 6 at 36,523,450 bp
  • A to G, chromosome 6 at 93,685,603 bp
  • G to A, chromosome 6 at 124,540,953 bp
  • T to A, chromosome 6 at 124,631,164 bp
  • A to T, chromosome 6 at 124,857,194 bp
  • A to T, chromosome 7 at 4,523,687 bp
  • A to T, chromosome 7 at 12,670,565 bp
  • A to G, chromosome 7 at 44,943,988 bp
  • G to A, chromosome 7 at 99,472,887 bp
  • A to G, chromosome 7 at 104,357,371 bp
  • TTCCTC to TTC, chromosome 7 at 119,951,021 bp
  • T to A, chromosome 7 at 139,875,280 bp
  • A to G, chromosome 8 at 3,603,980 bp
  • T to A, chromosome 8 at 26,044,327 bp
  • T to A, chromosome 8 at 56,307,667 bp
  • T to C, chromosome 9 at 104,190,385 bp
  • T to A, chromosome 9 at 109,386,761 bp
  • A to G, chromosome 10 at 105,271,441 bp
  • A to G, chromosome 10 at 123,181,742 bp
  • A to T, chromosome 10 at 129,704,887 bp
  • T to C, chromosome 11 at 23,001,354 bp
  • T to C, chromosome 11 at 46,469,516 bp
  • A to G, chromosome 11 at 52,213,054 bp
  • A to G, chromosome 11 at 59,038,609 bp
  • A to T, chromosome 11 at 59,758,554 bp
  • A to T, chromosome 11 at 105,998,362 bp
  • G to T, chromosome 11 at 117,815,922 bp
  • A to T, chromosome 12 at 40,817,736 bp
  • A to C, chromosome 12 at 51,773,835 bp
  • G to T, chromosome 12 at 76,028,124 bp
  • G to T, chromosome 12 at 83,876,747 bp
  • A to T, chromosome 12 at 101,602,766 bp
  • T to C, chromosome 13 at 23,757,232 bp
  • T to C, chromosome 13 at 63,565,454 bp
  • T to A, chromosome 13 at 68,738,588 bp
  • T to C, chromosome 13 at 98,355,041 bp
  • T to A, chromosome 14 at 32,463,923 bp
  • A to G, chromosome 15 at 75,747,778 bp
  • A to T, chromosome 17 at 29,282,308 bp
  • G to T, chromosome 19 at 10,053,033 bp
  • A to G, chromosome 19 at 42,115,038 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5846 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044064-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.