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Strain Name:
C57BL/6J-MtgxR6239Btlr/Mmmh
Stock Number:
044363-MU
Citation ID:
RRID:MMRRC_044363-MU
Other Names:
R6239 (G1)
Major Collection:

Strain Information

Ttr
Name: transthyretin
Synonyms: prealbumin
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 22139
VEGA: 18
Homologene: 317
Pald1
Name: phosphatase domain containing, paladin 1
Synonyms: paladin, X99384
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 27355
VEGA: 10
Homologene: 8453
Il4i1
Name: interleukin 4 induced 1
Synonyms: Fig1-ps, Fig1, H46, H4, H-46, H-4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14204
Homologene: 22567
Dnmbp
Name: dynamin binding protein
Synonyms: 2410003M15Rik, Tuba, 2410003L07Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71972
Homologene: 9061
Ptpn3
Name: protein tyrosine phosphatase, non-receptor type 3
Synonyms: PTPCL, 9530011I20Rik, PTP-H1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 545622
HGNC: HGNC:9655
Homologene: 74451
Mast4
Name: microtubule associated serine/threonine kinase family member 4
Synonyms: 4930420O11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 328329
Homologene: 42094
Fus
Name: fused in sarcoma
Synonyms: translocated in liposarcoma, Tls, hnRNP P2, pigpen, D930039C12Rik, D430004D17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233908
HGNC: HGNC:4010
Homologene: 134091
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 58,305,391 bp
  • G to A, chromosome 1 at 74,384,115 bp
  • T to A, chromosome 1 at 78,696,465 bp
  • T to C, chromosome 1 at 92,054,972 bp
  • A to G, chromosome 1 at 164,050,808 bp
  • A to T, chromosome 1 at 165,351,270 bp
  • A to T, chromosome 1 at 166,098,819 bp
  • A to T, chromosome 1 at 192,595,087 bp
  • T to C, chromosome 2 at 52,273,988 bp
  • A to G, chromosome 2 at 91,995,403 bp
  • A to T, chromosome 2 at 125,579,412 bp
  • T to C, chromosome 2 at 165,406,697 bp
  • A to T, chromosome 3 at 93,201,272 bp
  • A to G, chromosome 3 at 98,231,284 bp
  • T to A, chromosome 3 at 106,494,877 bp
  • A to T, chromosome 3 at 129,800,346 bp
  • C to A, chromosome 4 at 57,249,981 bp
  • A to C, chromosome 4 at 103,066,443 bp
  • T to A, chromosome 4 at 133,268,295 bp
  • A to G, chromosome 4 at 148,938,277 bp
  • G to T, chromosome 5 at 86,533,777 bp
  • G to A, chromosome 5 at 114,644,826 bp
  • A to G, chromosome 6 at 39,488,816 bp
  • A to T, chromosome 6 at 137,380,608 bp
  • G to A, chromosome 7 at 5,454,540 bp
  • C to A, chromosome 7 at 13,261,331 bp
  • A to T, chromosome 7 at 27,157,475 bp
  • A to G, chromosome 7 at 44,840,412 bp
  • C to T, chromosome 7 at 119,477,297 bp
  • A to T, chromosome 7 at 120,794,234 bp
  • C to T, chromosome 7 at 127,981,434 bp
  • A to T, chromosome 8 at 35,778,764 bp
  • G to A, chromosome 8 at 47,529,494 bp
  • G to T, chromosome 8 at 84,908,879 bp
  • T to C, chromosome 9 at 15,322,631 bp
  • T to C, chromosome 9 at 44,819,796 bp
  • A to G, chromosome 9 at 56,888,182 bp
  • T to A, chromosome 9 at 57,702,078 bp
  • T to C, chromosome 10 at 61,321,131 bp
  • A to G, chromosome 10 at 75,585,681 bp
  • A to T, chromosome 10 at 76,222,591 bp
  • A to G, chromosome 11 at 4,218,967 bp
  • T to C, chromosome 11 at 29,749,275 bp
  • C to T, chromosome 11 at 95,876,239 bp
  • G to A, chromosome 11 at 97,052,824 bp
  • C to T, chromosome 11 at 102,465,318 bp
  • A to T, chromosome 12 at 87,464,628 bp
  • A to T, chromosome 12 at 104,780,845 bp
  • A to G, chromosome 13 at 102,736,209 bp
  • A to T, chromosome 15 at 39,198,363 bp
  • T to C, chromosome 15 at 77,526,431 bp
  • T to A, chromosome 15 at 85,021,314 bp
  • T to C, chromosome 15 at 99,280,434 bp
  • T to C, chromosome 17 at 25,028,972 bp
  • C to T, chromosome 17 at 30,810,359 bp
  • T to C, chromosome 17 at 87,411,239 bp
  • T to C, chromosome 18 at 20,673,635 bp
  • T to C, chromosome 18 at 38,282,401 bp
  • T to A, chromosome 18 at 39,773,838 bp
  • G to T, chromosome 18 at 82,657,104 bp
  • C to T, chromosome 19 at 43,848,185 bp
  • A to G, chromosome 19 at 46,259,696 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6239 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044363-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.