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Strain Name:
C57BL/6J-MtgxR6281Btlr/Mmmh
Stock Number:
044451-MU
Citation ID:
RRID:MMRRC_044451-MU
Other Names:
R6281 (G1)
Major Collection:

Strain Information

Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: N-CoR, Rxrip13, A230020K14Rik, 5730405M06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
Znfx1
Name: zinc finger, NFX1-type containing 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 98999
Homologene: 10877
Ankib1
Name: ankyrin repeat and IBR domain containing 1
Synonyms: 2310061P20Rik, 4631416I11Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70797
Homologene: 19076
Rad23a
Name: RAD23 homolog A, nucleotide excision repair protein
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19358
HGNC: HGNC:9812
Homologene: 48322
Muc2
Name: mucin 2
Synonyms: 2010015E03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17831
HGNC: HGNC:7512
Homologene: 136755
Zfyve27
Name: zinc finger, FYVE domain containing 27
Synonyms: 9530077C24Rik, 2210011N02Rik, protrudin
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 319740
Homologene: 16939
Fancm
Name: Fanconi anemia, complementation group M
Synonyms: C730036B14Rik, D12Ertd364e
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104806
VEGA: 12
Homologene: 35378
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 19,104,478 bp
  • T to A, chromosome 1 at 37,123,982 bp
  • C to T, chromosome 1 at 183,322,290 bp
  • C to T, chromosome 2 at 76,941,828 bp
  • A to T, chromosome 2 at 103,471,769 bp
  • T to C, chromosome 2 at 111,369,549 bp
  • T to C, chromosome 2 at 154,206,465 bp
  • A to G, chromosome 2 at 167,055,885 bp
  • C to T, chromosome 3 at 146,624,577 bp
  • T to G, chromosome 4 at 44,691,955 bp
  • T to C, chromosome 5 at 3,701,965 bp
  • T to C, chromosome 5 at 71,034,762 bp
  • T to A, chromosome 5 at 83,294,606 bp
  • T to C, chromosome 5 at 145,165,071 bp
  • GAT to GATCAT, chromosome 6 at 4,756,449 bp
  • T to G, chromosome 6 at 49,469,180 bp
  • T to A, chromosome 6 at 108,664,462 bp
  • A to G, chromosome 7 at 3,911,973 bp
  • T to G, chromosome 7 at 108,630,402 bp
  • C to G, chromosome 7 at 141,752,403 bp
  • A to T, chromosome 8 at 84,838,110 bp
  • C to A, chromosome 8 at 121,531,881 bp
  • C to A, chromosome 8 at 122,633,409 bp
  • T to A, chromosome 10 at 22,665,320 bp
  • G to A, chromosome 10 at 93,973,946 bp
  • T to C, chromosome 11 at 62,373,545 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • T to C, chromosome 12 at 24,957,679 bp
  • T to C, chromosome 12 at 65,088,270 bp
  • C to A, chromosome 12 at 115,758,403 bp
  • T to A, chromosome 13 at 23,856,799 bp
  • G to A, chromosome 13 at 93,663,160 bp
  • T to A, chromosome 14 at 7,159,254 bp
  • G to A, chromosome 14 at 20,714,640 bp
  • G to T, chromosome 15 at 84,854,745 bp
  • A to T, chromosome 16 at 23,118,066 bp
  • A to G, chromosome 16 at 58,718,594 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • A to T, chromosome 18 at 37,757,373 bp
  • T to A, chromosome 19 at 42,182,755 bp
  • T to C, chromosome 19 at 46,724,923 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6281 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044451-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.