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Strain Name:
C57BL/6J-MtgxR6353Btlr/Mmmh
Stock Number:
044505-MU
Citation ID:
RRID:MMRRC_044505-MU
Other Names:
R6353 (G1)
Major Collection:

Strain Information

Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Mcoln3
Name: mucolipin 3
Synonyms: varitint-waddler, Va, 6720490O21Rik, TRPML3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 171166
Homologene: 10118
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, D230038D11Rik, 9530077A04Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Nphs1
Name: nephrosis 1, nephrin
Synonyms: nephrin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54631
HGNC: HGNC:7908
Homologene: 20974
Itga5
Name: integrin alpha 5 (fibronectin receptor alpha)
Synonyms: Fnra, Cd49e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16402
VEGA: 15
HGNC: HGNC:6141
Homologene: 20508
Ttc39b
Name: tetratricopeptide repeat domain 39B
Synonyms: 9130422G05Rik, 1810054D07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69863
Homologene: 25228
Usp32
Name: ubiquitin specific peptidase 32
Synonyms: 6430526O11Rik, 2900074J03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237898
Homologene: 13066
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 43,797,250 bp
  • T to C, chromosome 1 at 51,181,826 bp
  • T to C, chromosome 1 at 53,347,779 bp
  • T to C, chromosome 1 at 74,280,828 bp
  • A to G, chromosome 1 at 163,704,111 bp
  • A to T, chromosome 2 at 76,841,809 bp
  • G to T, chromosome 2 at 119,776,896 bp
  • A to T, chromosome 3 at 30,896,022 bp
  • A to T, chromosome 3 at 40,653,708 bp
  • A to T, chromosome 3 at 59,752,108 bp
  • A to T, chromosome 3 at 79,941,340 bp
  • T to C, chromosome 3 at 146,131,154 bp
  • G to A, chromosome 4 at 40,186,367 bp
  • C to G, chromosome 4 at 83,230,493 bp
  • T to C, chromosome 4 at 96,585,898 bp
  • C to T, chromosome 4 at 149,160,752 bp
  • T to A, chromosome 5 at 23,493,245 bp
  • A to G, chromosome 5 at 109,340,253 bp
  • C to T, chromosome 5 at 144,282,088 bp
  • C to A, chromosome 6 at 43,116,136 bp
  • T to A, chromosome 6 at 128,982,932 bp
  • A to G, chromosome 7 at 23,541,519 bp
  • A to G, chromosome 7 at 30,474,544 bp
  • C to A, chromosome 7 at 79,935,345 bp
  • G to A, chromosome 7 at 105,734,118 bp
  • T to C, chromosome 8 at 123,893,687 bp
  • T to A, chromosome 9 at 21,679,149 bp
  • T to C, chromosome 9 at 36,723,959 bp
  • T to C, chromosome 9 at 38,498,816 bp
  • C to A, chromosome 9 at 53,414,342 bp
  • T to C, chromosome 9 at 57,716,237 bp
  • A to G, chromosome 9 at 114,597,546 bp
  • T to C, chromosome 11 at 85,022,281 bp
  • A to T, chromosome 12 at 8,009,421 bp
  • G to A, chromosome 12 at 85,301,829 bp
  • A to G, chromosome 13 at 27,773,726 bp
  • A to G, chromosome 14 at 16,416,671 bp
  • T to C, chromosome 14 at 55,894,303 bp
  • T to C, chromosome 15 at 44,420,762 bp
  • T to C, chromosome 15 at 103,352,523 bp
  • A to T, chromosome 17 at 24,442,245 bp
  • A to G, chromosome 19 at 34,234,120 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6353 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044505-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.