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Strain Name:
C57BL/6J-MtgxR6405Btlr/Mmmh
Stock Number:
044550-MU
Citation ID:
RRID:MMRRC_044550-MU
Other Names:
R6405 (G1)
Major Collection:

Strain Information

Wnt5b
Name: wingless-type MMTV integration site family, member 5B
Synonyms: Wnt-5b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22419
Homologene: 22530
Dhx35
Name: DEAH-box helicase 35
Synonyms: Ddx35, 1200009D07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71715
Homologene: 6406
Inpp5k
Name: inositol polyphosphate 5-phosphatase K
Synonyms: C62, PI-5-phosphatase related, putative PI-5-phosphatase, Pps
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19062
Homologene: 75059
Lgals9
Name: lectin, galactose binding, soluble 9
Synonyms: LGALS35, galectin-9, gal-9, Lgals5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16859
Homologene: 134662
Asxl2
Name: ASXL transcriptional regulator 2
Synonyms: 4930556B16Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 75302
Homologene: 10102
Tmem87a
Name: transmembrane protein 87A
Synonyms: A930025J12Rik, Elkin1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 211499
Homologene: 9165
Arhgap32
Name: Rho GTPase activating protein 32
Synonyms: p200RhoGAP, GC-GAP, PX-RICS, 3426406O18Rik, Grit
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330914
Homologene: 8812
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,345,771 bp
  • A to T, chromosome 1 at 53,347,995 bp
  • G to T, chromosome 2 at 32,781,396 bp
  • A to G, chromosome 2 at 82,990,086 bp
  • A to G, chromosome 2 at 120,379,750 bp
  • T to A, chromosome 2 at 158,794,919 bp
  • A to G, chromosome 3 at 83,354,263 bp
  • A to G, chromosome 3 at 122,173,662 bp
  • T to C, chromosome 4 at 44,926,032 bp
  • A to T, chromosome 4 at 117,000,624 bp
  • A to T, chromosome 5 at 145,862,420 bp
  • C to CTCG, chromosome 6 at 4,756,453 bp
  • G to T, chromosome 6 at 41,674,668 bp
  • TG to TGG, chromosome 6 at 108,664,857 bp
  • T to C, chromosome 6 at 119,433,496 bp
  • T to A, chromosome 7 at 27,172,954 bp
  • A to G, chromosome 7 at 35,372,439 bp
  • T to C, chromosome 7 at 35,454,639 bp
  • T to A, chromosome 7 at 64,372,486 bp
  • T to A, chromosome 8 at 45,941,697 bp
  • T to C, chromosome 8 at 71,483,030 bp
  • T to A, chromosome 9 at 32,248,488 bp
  • A to T, chromosome 10 at 60,716,026 bp
  • T to C, chromosome 11 at 36,864,859 bp
  • T to C, chromosome 11 at 75,633,178 bp
  • C to A, chromosome 11 at 78,971,385 bp
  • T to A, chromosome 12 at 3,493,758 bp
  • T to C, chromosome 12 at 103,168,336 bp
  • A to T, chromosome 12 at 112,773,337 bp
  • T to C, chromosome 14 at 47,243,867 bp
  • T to C, chromosome 15 at 47,820,371 bp
  • T to G, chromosome 15 at 98,480,751 bp
  • T to C, chromosome 16 at 4,851,878 bp
  • CGTCTGGATG to CG, chromosome 16 at 31,956,343 bp
  • C to T, chromosome 16 at 96,678,425 bp
  • A to C, chromosome 17 at 20,279,099 bp
  • G to T, chromosome 17 at 22,618,235 bp
  • A to G, chromosome 17 at 37,660,123 bp
  • T to A, chromosome 18 at 10,501,076 bp
  • C to A, chromosome 18 at 33,464,305 bp
  • A to G, chromosome 18 at 74,359,606 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6405 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044550-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.