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Strain Name:
C57BL/6J-MtgxR6493Btlr/Mmmh
Stock Number:
044625-MU
Citation ID:
RRID:MMRRC_044625-MU
Other Names:
R6493 (G1)
Major Collection:

Strain Information

Plin2
Name: perilipin 2
Synonyms: Adrp, adipophilin, ADPH, Adfp
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11520
HGNC: HGNC:248
Homologene: 872
Hsd17b12
Name: hydroxysteroid (17-beta) dehydrogenase 12
Synonyms: KIK-I, 2610510O05Rik, keratoadhesin, keratonectin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56348
Homologene: 95094
Kcnj8
Name: potassium inwardly-rectifying channel, subfamily J, member 8
Synonyms: Kir6.1, sltr, slmbr, gnite
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16523
HGNC: HGNC:6269
Homologene: 3654
Shroom3
Name: shroom family member 3
Synonyms: D5Ertd287e, Shrm, Shrm3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 27428
Homologene: 9263
Tut4
Name: terminal uridylyl transferase 4
Synonyms: 6030404K05Rik, 9230115F04Rik, Tent3a, Zcchc11
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230594
Homologene: 35279
Parn
Name: poly(A)-specific ribonuclease (deadenylation nuclease)
Synonyms: DAN, 1200003I18Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74108
VEGA: 16
HGNC: HGNC:8609
Homologene: 31098
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 19,104,478 bp
  • C to T, chromosome 1 at 133,679,792 bp
  • A to T, chromosome 1 at 156,380,813 bp
  • T to C, chromosome 2 at 26,472,098 bp
  • T to C, chromosome 2 at 76,741,997 bp
  • T to C, chromosome 2 at 86,483,221 bp
  • G to A, chromosome 2 at 90,265,708 bp
  • A to T, chromosome 2 at 94,043,883 bp
  • G to A, chromosome 3 at 38,890,887 bp
  • G to A, chromosome 3 at 97,587,167 bp
  • A to T, chromosome 3 at 103,736,207 bp
  • T to C, chromosome 3 at 129,719,088 bp
  • T to A, chromosome 3 at 144,957,298 bp
  • A to T, chromosome 4 at 48,436,450 bp
  • G to A, chromosome 4 at 86,661,987 bp
  • A to G, chromosome 4 at 108,526,805 bp
  • A to G, chromosome 4 at 109,368,948 bp
  • A to T, chromosome 4 at 124,857,171 bp
  • G to A, chromosome 5 at 8,824,698 bp
  • A to G, chromosome 5 at 36,486,252 bp
  • C to A, chromosome 5 at 92,941,561 bp
  • A to G, chromosome 5 at 95,270,825 bp
  • A to G, chromosome 5 at 122,106,728 bp
  • T to C, chromosome 5 at 144,209,974 bp
  • C to T, chromosome 6 at 31,525,046 bp
  • T to C, chromosome 6 at 142,566,047 bp
  • T to G, chromosome 7 at 4,580,990 bp
  • T to A, chromosome 7 at 26,382,387 bp
  • T to C, chromosome 7 at 66,743,720 bp
  • T to G, chromosome 7 at 86,268,882 bp
  • G to T, chromosome 7 at 102,724,080 bp
  • A to G, chromosome 7 at 102,782,396 bp
  • A to G, chromosome 7 at 110,795,811 bp
  • T to G, chromosome 9 at 75,635,702 bp
  • T to C, chromosome 10 at 23,985,123 bp
  • T to C, chromosome 10 at 24,185,071 bp
  • C to T, chromosome 11 at 21,711,631 bp
  • A to G, chromosome 11 at 67,258,629 bp
  • G to A, chromosome 11 at 72,913,303 bp
  • T to C, chromosome 11 at 73,353,746 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • T to A, chromosome 11 at 110,293,878 bp
  • G to A, chromosome 12 at 65,097,488 bp
  • A to C, chromosome 12 at 72,308,329 bp
  • C to T, chromosome 12 at 76,386,473 bp
  • T to A, chromosome 13 at 27,507,352 bp
  • A to C, chromosome 16 at 13,656,925 bp
  • A to C, chromosome 16 at 22,822,466 bp
  • T to C, chromosome 17 at 3,171,119 bp
  • T to A, chromosome 17 at 33,912,371 bp
  • G to A, chromosome 18 at 12,482,148 bp
  • T to C, chromosome 18 at 37,144,705 bp
  • G to A, chromosome 18 at 41,998,701 bp
  • T to C, chromosome 18 at 74,948,024 bp
  • G to T, chromosome 19 at 6,318,455 bp
  • A to G, chromosome 19 at 8,839,774 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6493 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044625-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.