Strain Name:
C57BL/6J-MtgxR6503Btlr/Mmmh
Stock Number:
044635-MU
Citation ID:
RRID:MMRRC_044635-MU
Other Names:
R6503 (G1)
Major Collection:

Strain Information

Arhgef7
Name: Rho guanine nucleotide exchange factor
Synonyms: Cool, PIX, betaPix-c, cool-1, betaPix, betaPix-b, p85SPR, Pak interacting exchange factor
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54126
Homologene: 2895
Enah
Name: ENAH actin regulator
Synonyms: Mena, Ndpp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13800
Homologene: 134005
Anxa4
Name: annexin A4
Synonyms: Anx4, annexin IV, AIV, Xanx-4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11746
HGNC: HGNC:542
Homologene: 68164
Thap1
Name: THAP domain containing, apoptosis associated protein 1
Synonyms: 4833431A01Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 73754
Homologene: 10005
Syngap1
Name: synaptic Ras GTPase activating protein 1 homolog (rat)
Synonyms: Syngap
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240057
Homologene: 84739
Epha6
Name: Eph receptor A6
Synonyms: Hek12, m-ehk2, Ehk2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13840
Homologene: 56396
Rb1
Name: RB transcriptional corepressor 1
Synonyms: Rb, retinoblastoma 1, pRb, Rb-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19645
HGNC: HGNC:9884
Homologene: 272
Tmem131l
Name: transmembrane 131 like
Synonyms: D930015E06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229473
Homologene: 9057
Jag1
Name: jagged 1
Synonyms: Ozz, ABE2, Headturner, Htu, Serrate-1, Gsfabe2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16449
HGNC: HGNC:6188
Homologene: 180
Stag3
Name: STAG3 cohesin complex component
Synonyms: SA-2, stromalin 3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 50878
Homologene: 40844
Fat4
Name: FAT atypical cadherin 4
Synonyms: 6030410K14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329628
Homologene: 14377
Oas1e
Name: 2'-5' oligoadenylate synthetase 1E
Synonyms: 2'-5' oligoadenylate synthetase-like 7
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231699
HGNC: HGNC:8086
Homologene: 110815
Parp10
Name: poly (ADP-ribose) polymerase family, member 10
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 671535
Homologene: 53133
Pcdha1
Name: protocadherin alpha 1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 116731
HGNC: HGNC:8663
Homologene: 75093
St18
Name: suppression of tumorigenicity 18
Synonyms: Nzf3, Myt3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240690
Homologene: 8792
Sacs
Name: sacsin
Synonyms: E130115J16Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50720
Mettl18
Name: methyltransferase like 18
Synonyms: 2810422O20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 69962
Homologene: 32680
Acan
Name: aggrecan
Synonyms: Agc1, Cspg1, b2b183Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11595
HGNC: HGNC:319
Homologene: 137204
Krt8
Name: keratin 8
Synonyms: K8, EndoA, cytokeratin 8, cytokeratin8, cytokeratin-8, Krt2-8, Krt-2.8, Card2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16691
VEGA: 15
HGNC: HGNC:6446
Homologene: 55643
Dnaaf2
Name: dynein, axonemal assembly factor 2
Synonyms: kintoun, Ktu, 1110034A24Rik, 2810020C19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 109065
Homologene: 10026
Prb1c
Name: proline-rich protein BstNI subfamily 1C
Synonyms: Gm8882
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 667929
Cage1
Name: cancer antigen 1
Synonyms: Ctag3, 4933427I01Rik, CAGE1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 71213
Homologene: 18484
Or4a75
Name: olfactory receptor family 4 subfamily A member 75
Synonyms: GA_x6K02T2Q125-51059648-51058725, Olfr1248, MOR231-10
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258787
Homologene: 133613
Gulp1
Name: GULP, engulfment adaptor PTB domain containing 1
Synonyms: 5730529O06Rik, Ced6, 3110030A04Rik, CED-6, GULP
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70676
Homologene: 9467
Ntmt2
Name: N-terminal Xaa-Pro-Lys N-methyltransferase 2
Synonyms: LOC240879, Mettl11b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240879
Homologene: 28168
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 6,795,397 bp
  • A to T, chromosome 1 at 44,773,380 bp
  • T to A, chromosome 1 at 163,704,146 bp
  • T to C, chromosome 1 at 163,997,118 bp
  • C to T, chromosome 1 at 181,918,511 bp
  • A to G, chromosome 2 at 89,617,578 bp
  • C to T, chromosome 2 at 137,101,629 bp
  • A to G, chromosome 3 at 38,982,257 bp
  • A to G, chromosome 3 at 83,940,944 bp
  • T to C, chromosome 5 at 120,787,977 bp
  • T to C, chromosome 5 at 138,304,420 bp
  • A to G, chromosome 6 at 86,744,667 bp
  • C to A, chromosome 6 at 132,361,692 bp
  • G to T, chromosome 7 at 79,097,832 bp
  • A to G, chromosome 8 at 11,833,054 bp
  • CAGCATCTGCTCGGAGCA to CAGCA, chromosome 8 at 26,160,856 bp
  • A to T, chromosome 12 at 69,197,511 bp
  • G to A, chromosome 13 at 38,025,449 bp
  • T to A, chromosome 14 at 61,211,361 bp
  • T to C, chromosome 14 at 73,205,880 bp
  • G to A, chromosome 15 at 76,242,484 bp
  • T to A, chromosome 15 at 101,997,934 bp
  • T to A, chromosome 16 at 60,205,621 bp
  • A to G, chromosome 17 at 26,944,684 bp
  • G to A, chromosome 18 at 36,931,671 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6503 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044635-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.