Strain Name:
C57BL/6J-MtgxR6553Btlr/Mmmh
Stock Number:
044678-MU
Citation ID:
RRID:MMRRC_044678-MU
Other Names:
R6553 (G1)
Major Collection:

Strain Information

Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, 9530077A04Rik, D230038D11Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Chd1
Name: chromodomain helicase DNA binding protein 1
Synonyms: 4930525N21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12648
HGNC: HGNC:1915
Homologene: 68174
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Lsm3
Name: LSM3 homolog, U6 small nuclear RNA and mRNA degradation associated
Synonyms: 6030401D18Rik, SMX4, 1010001J12Rik, USS2, 2610005D18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67678
Homologene: 6548
Dis3l2
Name: DIS3 like 3'-5' exoribonuclease 2
Synonyms: 8030493P09Rik, 4930429A22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 208718
Homologene: 62417
Tmem161b
Name: transmembrane protein 161B
Synonyms: 2810446P07Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72745
Homologene: 14519
Dapk1
Name: death associated protein kinase 1
Synonyms: D13Ucla1, DAP-Kinase, 2310039H24Rik, 2810425C21Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69635
VEGA: 13
HGNC: HGNC:2674
Homologene: 3626
Gpr155
Name: G protein-coupled receptor 155
Synonyms: F730029F15Rik, 1110017O10Rik, PGR22, DEPDC3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68526
Homologene: 16584
Ptgs2
Name: prostaglandin-endoperoxide synthase 2
Synonyms: Tis10, PHS-2, cyclooxygenase-2, Pghs2, Cox-2, COX2, cyclooxygenase 2, PGHS-2, prostaglandin G/H synthase
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19225
HGNC: HGNC:9605
Homologene: 31000
Ciita
Name: class II transactivator
Synonyms: C2ta, Gm9475
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12265
VEGA: 16
HGNC: HGNC:7067
Homologene: 207
Exph5
Name: exophilin 5
Synonyms: B130009M24Rik, Slac2b, slac2-b, AC079869.22gm5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320051
Homologene: 9007
Vmn2r75
Name: vomeronasal 2, receptor 75
Synonyms: EG546981
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 546981
Homologene: 115466
Hltf
Name: helicase-like transcription factor
Synonyms: Snf2l3, Smarca3, P113
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20585
Homologene: 30136
Or6c6c
Name: olfactory receptor family 6 subfamily C member 6C
Synonyms: MOR110-7, Olfr804, GA_x6K02T2PULF-11383575-11384519
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258068
Homologene: 133581
Nprl3
Name: nitrogen permease regulator-like 3
Synonyms: m(alpha)RE, Mare, -14 gene, HS-40, Prox1, Phg, HS-26
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17168
Homologene: 8091
Cyp2c50
Name: cytochrome P450, family 2, subfamily c, polypeptide 50
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107141
Homologene: 137231
Zfp27
Name: zinc finger protein 27
Synonyms: mkr-4, Zfp-27
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22689
Homologene: 81823
Fcgbp
Name: Fc fragment of IgG binding protein
Synonyms: A430096B05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 215384
Homologene: 68369
Vmn2r97
Name: vomeronasal 2, receptor 97
Synonyms: EG627367
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627367
Homologene: 115024
Zpld2
Name: zona pellucida like domain containing 2
Synonyms: Gm7534
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 665186
Homologene: 104039
Asic5
Name: acid-sensing ion channel family member 5
Synonyms: brain-liver-intestine amiloride-sensitive sodium channel, Accn5, BLINaC
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 58170
Homologene: 41171
Speer4f2
Name: spermatogenesis associated glutamate (E)-rich protein 4f2
Synonyms: Gm3495, Gm3535
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100041749
Gm5622
Name: predicted gene 5622
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 434459
Homologene: 115686
Adam1b
Name: a disintegrin and metallopeptidase domain 1b
Synonyms: PH-30 alpha, fertilin alpha, Ftna
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 280667
Homologene: 136485
Gm2888
Name: predicted gene 2888
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100040657
Homologene: 115686
Trav13n-3
Name: T cell receptor alpha variable 13N-3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 547328
Trav9d-4
Name: T cell receptor alpha variable 9D-4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 667574
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 86,745,494 bp
  • T to C, chromosome 1 at 150,103,987 bp
  • A to T, chromosome 2 at 73,349,645 bp
  • T to C, chromosome 3 at 20,072,394 bp
  • A to T, chromosome 3 at 82,009,466 bp
  • A to G, chromosome 4 at 134,202,056 bp
  • A to G, chromosome 5 at 17,374,422 bp
  • G to A, chromosome 5 at 23,463,026 bp
  • G to T, chromosome 5 at 121,501,187 bp
  • GATATATA to GATATATATA, chromosome 6 at 91,519,635 bp
  • A to T, chromosome 7 at 28,113,979 bp
  • G to A, chromosome 7 at 29,896,393 bp
  • T to A, chromosome 7 at 86,164,245 bp
  • A to G, chromosome 9 at 53,301,712 bp
  • A to G, chromosome 10 at 50,842,177 bp
  • C to T, chromosome 10 at 129,705,063 bp
  • C to T, chromosome 11 at 32,234,812 bp
  • T to A, chromosome 13 at 60,761,161 bp
  • C to A, chromosome 13 at 84,222,418 bp
  • A to G, chromosome 14 at 3,037,722 bp
  • A to G, chromosome 14 at 51,657,743 bp
  • A to T, chromosome 14 at 52,983,741 bp
  • A to G, chromosome 14 at 53,337,161 bp
  • T to A, chromosome 16 at 10,511,745 bp
  • A to G, chromosome 17 at 15,725,430 bp
  • G to A, chromosome 17 at 18,930,304 bp
  • A to G, chromosome 19 at 40,090,602 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6553 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044678-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.