Strain Name:
C57BL/6J-MtgxR6581Btlr/Mmmh
Stock Number:
044705-MU
Citation ID:
RRID:MMRRC_044705-MU
Other Names:
R6581 (G1)
Major Collection:

Strain Information

Itgb8
Name: integrin beta 8
Synonyms: 4832412O06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320910
HGNC: HGNC:6163
Homologene: 18567
Slc12a5
Name: solute carrier family 12, member 5
Synonyms: KCC2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 57138
Homologene: 10665
Etv5
Name: ets variant 5
Synonyms: 1110005E01Rik, erm, 8430401F14Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 104156
HGNC: HGNC:3494
Homologene: 3276
Luzp1
Name: leucine zipper protein 1
Synonyms: 2700072H04Rik, Luzp
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269593
Homologene: 11545
Tnpo2
Name: transportin 2 (importin 3, karyopherin beta 2b)
Synonyms: TRN2, 1110034O24Rik, Kpnb2b
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 212999
Homologene: 8381
Smyd5
Name: SET and MYND domain containing 5
Synonyms: NN8-4AG, Rai15, Rrg1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232187
Homologene: 6143
Mrtfa
Name: myocardin related transcription factor A
Synonyms: Mkl1, Bsac, Mal, MRTF-A
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223701
Homologene: 32487
Ms4a4d
Name: membrane-spanning 4-domains, subfamily A, member 4D
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 66607
Homologene: 113790
Itga9
Name: integrin alpha 9
Synonyms: 6720458D17Rik, 2610002H11Rik, D9Ertd428e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 104099
HGNC: HGNC:6145
Homologene: 1664
Helz2
Name: helicase with zinc finger 2, transcriptional coactivator
Synonyms: BC006779
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 229003
Homologene: 14118
Uchl4
Name: ubiquitin carboxyl-terminal esterase L4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 93841
VEGA: 9
Cdh17
Name: cadherin 17
Synonyms: BILL-cadherin, LI-cadherin, HPT-1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12557
HGNC: HGNC:1756
Homologene: 56859
Odad2
Name: outer dynein arm docking complex subunit 2
Synonyms: Armc4, 4930463I21Rik, b2b227.1Clo, b2b643Clo
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 74934
VEGA: 18
Homologene: 9992
Dpy19l1
Name: dpy-19 like C-mannosyltransferase 1
Synonyms: 1100001I19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244745
Homologene: 14762
Thbd
Name: thrombomodulin
Synonyms: CD141, TM
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21824
Homologene: 308
Aldh8a1
Name: aldehyde dehydrogenase 8 family, member A1
Synonyms: RALDH4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237320
Homologene: 23369
Dnajb7
Name: DnaJ heat shock protein family (Hsp40) member B7
Synonyms: mDj5, 4933424H20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 57755
Homologene: 77343
Tiam2
Name: T cell lymphoma invasion and metastasis 2
Synonyms: STEF, 3000002F19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 24001
VEGA: 17
Homologene: 40796
Spata18
Name: spermatogenesis associated 18
Synonyms: 1700067I02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 73472
Homologene: 17057
Or52a5b
Name: olfactory receptor family 52 subfamily A member 5B
Synonyms: Olfr69, MOR22-2, 3'[b]3, GA_x6K02T2PBJ9-6494485-6493535
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18370
Homologene: 128070
Vmn1r5
Name: vomeronasal 1 receptor 5
Synonyms: V1rc19
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 171192
Homologene: 128340
Gbp2b
Name: guanylate binding protein 2b
Synonyms: Gbp1, Gbp-1, Mag-1, Mpa1, Mpa-1, LIMIT
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14468
Homologene: 137233
Or3a1d
Name: olfactory receptor family 3 subfamily A member 1D
Synonyms: MOR255-3, GA_x6K02T2P1NL-4481525-4480578, Olfr411
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258704
Homologene: 74999
Prl7a1
Name: prolactin family 7, subfamily a, member 1
Synonyms: PLP-G, Prlpe, PLP-E, Prlpg
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19113
Homologene: 136279
Yaf2
Name: YY1 associated factor 2
Synonyms: 2810021M11Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 67057
Homologene: 4203
Or2f1
Name: olfactory receptor family 2 subfamily F member 1
Synonyms: GA_x6K02T2P3E9-4815856-4814903, Olfr453, MOR257-8P
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 258016
HGNC: HGNC:8246
Homologene: 128151
Vmn1r158
Name: vomeronasal 1 receptor 158
Synonyms: Gm16455
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043067
Homologene: 104166
Gm21103
Name: predicted gene, 21103
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 118567363
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 148,406,272 bp
  • T to C, chromosome 2 at 164,987,115 bp
  • A to G, chromosome 2 at 181,229,379 bp
  • T to A, chromosome 3 at 142,608,238 bp
  • A to T, chromosome 4 at 11,799,615 bp
  • A to G, chromosome 4 at 136,540,631 bp
  • G to T, chromosome 5 at 73,669,516 bp
  • T to C, chromosome 6 at 42,744,079 bp
  • T to C, chromosome 6 at 56,985,381 bp
  • G to A, chromosome 6 at 85,432,023 bp
  • G to A, chromosome 7 at 22,790,040 bp
  • T to C, chromosome 7 at 103,768,221 bp
  • C to A, chromosome 8 at 85,055,404 bp
  • T to A, chromosome 9 at 24,447,864 bp
  • A to T, chromosome 9 at 64,235,793 bp
  • A to T, chromosome 9 at 118,658,564 bp
  • G to A, chromosome 10 at 21,380,842 bp
  • A to G, chromosome 11 at 74,347,206 bp
  • A to T, chromosome 12 at 119,163,215 bp
  • A to T, chromosome 13 at 27,633,629 bp
  • A to T, chromosome 14 at 6,303,815 bp
  • A to G, chromosome 15 at 81,016,373 bp
  • T to A, chromosome 15 at 81,408,025 bp
  • T to C, chromosome 15 at 93,286,414 bp
  • C to T, chromosome 16 at 22,439,699 bp
  • CGGG to CGGGG, chromosome 17 at 3,414,622 bp
  • C to T, chromosome 18 at 7,129,560 bp
  • G to A, chromosome 19 at 11,554,840 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6581 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044705-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.