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Strain Name:
C57BL/6J-MtgxR6950Btlr/Mmmh
Stock Number:
045062-MU
Citation ID:
RRID:MMRRC_045062-MU
Other Names:
R6950 (G1)
Major Collection:

Strain Information

Phf14
Name: PHD finger protein 14
Synonyms: 1110001C23Rik, 4932409F11Rik, 5730446A07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75725
Homologene: 8775
Oxr1
Name: oxidation resistance 1
Synonyms: C7B, C7, 2210416C20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170719
VEGA: 15
Homologene: 24993
Gch1
Name: GTP cyclohydrolase 1
Synonyms: GTPCH, GTP-CH
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14528
VEGA: 14
HGNC: HGNC:4193
Homologene: 132
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Rapgef6
Name: Rap guanine nucleotide exchange factor (GEF) 6
Synonyms: PDZ-GEF2, RA-GEF-2, Pdzgef2, C030018K18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192786
Homologene: 22968
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238130
VEGA: 12
Homologene: 56680
Gapvd1
Name: GTPase activating protein and VPS9 domains 1
Synonyms: 2010005B09Rik, 4432404J10Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66691
Homologene: 32637
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 40,602,885 bp
  • T to G, chromosome 1 at 134,971,357 bp
  • A to G, chromosome 2 at 34,684,245 bp
  • A to G, chromosome 2 at 82,985,988 bp
  • T to A, chromosome 2 at 89,667,551 bp
  • T to A, chromosome 2 at 112,248,559 bp
  • T to C, chromosome 2 at 112,686,825 bp
  • T to C, chromosome 2 at 121,093,647 bp
  • C to T, chromosome 3 at 36,638,590 bp
  • T to C, chromosome 3 at 88,349,269 bp
  • T to A, chromosome 4 at 45,815,494 bp
  • A to T, chromosome 4 at 53,015,136 bp
  • T to A, chromosome 4 at 131,776,352 bp
  • A to T, chromosome 4 at 153,970,092 bp
  • G to T, chromosome 4 at 154,062,053 bp
  • A to T, chromosome 5 at 64,979,979 bp
  • A to G, chromosome 5 at 108,643,082 bp
  • A to T, chromosome 6 at 12,006,855 bp
  • A to G, chromosome 6 at 97,134,382 bp
  • C to T, chromosome 6 at 112,472,210 bp
  • T to G, chromosome 7 at 27,818,914 bp
  • A to G, chromosome 7 at 27,990,514 bp
  • A to T, chromosome 7 at 28,523,007 bp
  • C to A, chromosome 7 at 45,319,280 bp
  • A to T, chromosome 8 at 48,240,479 bp
  • G to T, chromosome 8 at 85,670,443 bp
  • G to T, chromosome 8 at 93,530,774 bp
  • A to T, chromosome 8 at 99,030,763 bp
  • G to T, chromosome 8 at 105,425,389 bp
  • A to G, chromosome 8 at 120,755,125 bp
  • A to T, chromosome 9 at 38,268,546 bp
  • C to T, chromosome 9 at 101,101,975 bp
  • T to A, chromosome 9 at 102,194,909 bp
  • T to A, chromosome 10 at 19,607,293 bp
  • T to A, chromosome 11 at 54,676,380 bp
  • C to T, chromosome 11 at 94,667,629 bp
  • A to G, chromosome 11 at 96,351,901 bp
  • T to G, chromosome 11 at 97,686,160 bp
  • T to C, chromosome 11 at 120,608,647 bp
  • A to G, chromosome 12 at 40,733,314 bp
  • T to C, chromosome 13 at 59,450,266 bp
  • A to G, chromosome 13 at 68,888,065 bp
  • C to A, chromosome 14 at 14,095,511 bp
  • A to T, chromosome 14 at 47,189,266 bp
  • C to A, chromosome 15 at 41,820,555 bp
  • T to C, chromosome 15 at 60,823,714 bp
  • T to C, chromosome 15 at 98,840,020 bp
  • T to A, chromosome 16 at 14,411,616 bp
  • T to A, chromosome 16 at 15,815,986 bp
  • T to C, chromosome 16 at 30,067,271 bp
  • C to T, chromosome 16 at 89,860,204 bp
  • T to C, chromosome 17 at 8,257,066 bp
  • T to C, chromosome 17 at 12,718,718 bp
  • T to C, chromosome 17 at 15,807,786 bp
  • C to T, chromosome 17 at 23,310,163 bp
  • T to G, chromosome 17 at 32,492,306 bp
  • T to C, chromosome 17 at 55,939,137 bp
  • A to C, chromosome 18 at 58,035,921 bp
  • A to T, chromosome 19 at 6,105,981 bp
  • A to G, chromosome 19 at 12,099,390 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6950 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045062-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.