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Strain Name:
C57BL/6J-MtgxR7229Btlr/Mmmh
Stock Number:
045301-MU
Citation ID:
RRID:MMRRC_045301-MU
Other Names:
R7229 (G1)
Major Collection:

Strain Information

Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Zmynd8
Name: zinc finger, MYND-type containing 8
Synonyms: 1110013E22Rik, 2010005I16Rik, ZMYND8, RACK7, 3632413B07Rik, Prkcbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228880
HGNC: HGNC:9397
Homologene: 32679
Eps8
Name: epidermal growth factor receptor pathway substrate 8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13860
HGNC: HGNC:3420
Homologene: 3272
Foxp1
Name: forkhead box P1
Synonyms: 4932443N09Rik, 3110052D19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108655
HGNC: HGNC:3823
Homologene: 13092
Cep83
Name: centrosomal protein 83
Synonyms: 2600001G24Rik, 5730513H21Rik, 4921537D05Rik, Ccdc41
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77048
VEGA: 10
Homologene: 9396
Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74996
Homologene: 9929
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 87,209,444 bp
  • T to C, chromosome 1 at 97,785,151 bp
  • A to T, chromosome 1 at 128,040,893 bp
  • A to T, chromosome 1 at 161,247,297 bp
  • G to A, chromosome 1 at 183,291,959 bp
  • A to T, chromosome 2 at 67,525,551 bp
  • G to T, chromosome 2 at 76,846,781 bp
  • A to G, chromosome 2 at 82,258,625 bp
  • G to A, chromosome 2 at 85,196,440 bp
  • T to C, chromosome 2 at 120,427,775 bp
  • G to A, chromosome 2 at 121,528,920 bp
  • A to T, chromosome 2 at 122,108,104 bp
  • A to G, chromosome 2 at 165,858,053 bp
  • T to C, chromosome 3 at 84,977,369 bp
  • A to G, chromosome 3 at 87,750,289 bp
  • T to C, chromosome 3 at 145,084,108 bp
  • T to C, chromosome 4 at 101,890,242 bp
  • C to T, chromosome 4 at 135,856,217 bp
  • T to C, chromosome 5 at 20,465,588 bp
  • T to C, chromosome 5 at 45,584,175 bp
  • T to C, chromosome 5 at 72,618,249 bp
  • C to T, chromosome 5 at 146,455,653 bp
  • A to G, chromosome 6 at 57,390,098 bp
  • A to G, chromosome 6 at 72,594,753 bp
  • G to A, chromosome 6 at 84,577,150 bp
  • A to T, chromosome 6 at 98,935,412 bp
  • G to A, chromosome 6 at 115,791,192 bp
  • T to C, chromosome 6 at 130,191,261 bp
  • A to G, chromosome 6 at 137,539,356 bp
  • T to A, chromosome 7 at 24,987,985 bp
  • T to A, chromosome 7 at 35,480,280 bp
  • T to G, chromosome 7 at 106,818,995 bp
  • T to C, chromosome 7 at 112,092,877 bp
  • A to T, chromosome 7 at 118,176,955 bp
  • A to T, chromosome 8 at 70,100,311 bp
  • A to T, chromosome 8 at 71,511,577 bp
  • G to A, chromosome 8 at 92,831,786 bp
  • T to G, chromosome 8 at 105,946,737 bp
  • A to T, chromosome 8 at 107,056,352 bp
  • T to C, chromosome 9 at 21,037,001 bp
  • T to A, chromosome 9 at 56,057,479 bp
  • A to G, chromosome 9 at 58,853,399 bp
  • T to C, chromosome 10 at 9,798,187 bp
  • C to A, chromosome 10 at 24,902,004 bp
  • C to A, chromosome 10 at 26,292,662 bp
  • A to T, chromosome 10 at 49,101,416 bp
  • A to G, chromosome 10 at 94,719,665 bp
  • T to A, chromosome 10 at 128,488,048 bp
  • A to C, chromosome 11 at 5,803,013 bp
  • T to C, chromosome 11 at 50,791,820 bp
  • A to T, chromosome 11 at 60,496,495 bp
  • T to C, chromosome 11 at 67,026,163 bp
  • A to G, chromosome 11 at 74,682,278 bp
  • G to A, chromosome 12 at 21,296,737 bp
  • A to T, chromosome 12 at 26,454,123 bp
  • G to A, chromosome 12 at 117,227,225 bp
  • A to T, chromosome 13 at 13,643,509 bp
  • G to A, chromosome 13 at 89,705,270 bp
  • T to C, chromosome 13 at 98,692,625 bp
  • T to C, chromosome 14 at 54,595,352 bp
  • G to A, chromosome 14 at 54,625,951 bp
  • G to A, chromosome 15 at 27,658,187 bp
  • A to G, chromosome 15 at 101,947,394 bp
  • A to G, chromosome 16 at 29,420,905 bp
  • T to C, chromosome 16 at 89,570,925 bp
  • A to G, chromosome 16 at 91,499,556 bp
  • C to T, chromosome 17 at 6,231,780 bp
  • A to T, chromosome 17 at 20,540,963 bp
  • A to G, chromosome 17 at 29,695,424 bp
  • A to G, chromosome 17 at 34,977,255 bp
  • A to T, chromosome 17 at 67,752,446 bp
  • T to A, chromosome 18 at 37,266,687 bp
  • A to T, chromosome 18 at 77,764,134 bp
  • A to G, chromosome 18 at 82,405,664 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7229 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045301-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.