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Strain Name:
C57BL/6J-MtgxR7421Btlr/Mmmh
Stock Number:
045499-MU
Citation ID:
RRID:MMRRC_045499-MU
Other Names:
R7421 (G1)
Major Collection:

Strain Information

Itpk1
Name: inositol 1,3,4-triphosphate 5/6 kinase
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217837
HGNC: HGNC:6177
Homologene: 8588
Wasf2
Name: WASP family, member 2
Synonyms: D4Ertd13e, WAVE2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242687
Homologene: 86743
Pik3r1
Name: phosphoinositide-3-kinase regulatory subunit 1
Synonyms: p85alpha, p50alpha, p55alpha, PI3K
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18708
HGNC: HGNC:8979
Homologene: 7889
Cnmd
Name: chondromodulin
Synonyms: Chondromodulin 1, ChM-I, Bricd3, Chmd, Lect1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16840
Homologene: 5095
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Chd1
Name: chromodomain helicase DNA binding protein 1
Synonyms: 4930525N21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12648
HGNC: HGNC:1915
Homologene: 68174
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 60,763,300 bp
  • A to C, chromosome 1 at 71,247,136 bp
  • A to G, chromosome 1 at 136,086,802 bp
  • G to T, chromosome 2 at 58,771,574 bp
  • A to C, chromosome 2 at 66,675,532 bp
  • T to A, chromosome 2 at 89,630,571 bp
  • A to G, chromosome 2 at 120,307,256 bp
  • T to A, chromosome 2 at 122,323,230 bp
  • G to T, chromosome 2 at 125,110,617 bp
  • T to C, chromosome 2 at 156,555,995 bp
  • T to A, chromosome 3 at 154,733,561 bp
  • A to G, chromosome 4 at 21,743,291 bp
  • A to G, chromosome 4 at 133,185,101 bp
  • T to A, chromosome 5 at 137,354,425 bp
  • A to C, chromosome 6 at 27,762,477 bp
  • A to T, chromosome 6 at 42,596,842 bp
  • T to C, chromosome 7 at 44,815,138 bp
  • T to C, chromosome 7 at 66,660,120 bp
  • C to T, chromosome 7 at 101,832,937 bp
  • G to A, chromosome 7 at 128,140,432 bp
  • T to C, chromosome 7 at 141,748,126 bp
  • T to A, chromosome 8 at 3,690,335 bp
  • A to T, chromosome 8 at 13,405,316 bp
  • C to T, chromosome 8 at 93,215,075 bp
  • A to G, chromosome 9 at 13,810,673 bp
  • A to G, chromosome 9 at 96,611,447 bp
  • G to A, chromosome 9 at 106,020,795 bp
  • G to A, chromosome 10 at 23,865,768 bp
  • G to A, chromosome 10 at 74,454,065 bp
  • A to G, chromosome 10 at 76,221,078 bp
  • T to A, chromosome 10 at 80,232,753 bp
  • G to C, chromosome 11 at 9,510,463 bp
  • A to T, chromosome 11 at 58,890,107 bp
  • G to A, chromosome 11 at 69,492,805 bp
  • G to T, chromosome 11 at 73,955,509 bp
  • G to A, chromosome 11 at 76,694,225 bp
  • A to T, chromosome 11 at 82,981,371 bp
  • A to G, chromosome 11 at 82,987,736 bp
  • A to G, chromosome 11 at 83,121,141 bp
  • A to G, chromosome 11 at 84,363,736 bp
  • G to T, chromosome 11 at 95,068,855 bp
  • A to T, chromosome 11 at 100,135,560 bp
  • T to C, chromosome 11 at 115,604,891 bp
  • G to T, chromosome 11 at 121,624,178 bp
  • C to T, chromosome 12 at 52,518,000 bp
  • A to T, chromosome 12 at 102,574,065 bp
  • G to T, chromosome 13 at 19,190,644 bp
  • A to C, chromosome 13 at 76,062,515 bp
  • A to T, chromosome 13 at 76,148,825 bp
  • A to G, chromosome 13 at 101,689,136 bp
  • T to C, chromosome 14 at 79,645,507 bp
  • A to G, chromosome 15 at 58,134,926 bp
  • G to A, chromosome 15 at 89,371,720 bp
  • A to T, chromosome 16 at 3,940,793 bp
  • G to T, chromosome 16 at 4,668,751 bp
  • A to G, chromosome 17 at 15,749,398 bp
  • T to C, chromosome 17 at 26,939,457 bp
  • A to G, chromosome 17 at 35,425,228 bp
  • T to A, chromosome 18 at 5,056,109 bp
  • C to T, chromosome 19 at 24,709,299 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7421 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045499-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.