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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR7648Btlr/Mmmh
Stock Number:
045726-MU
Citation ID:
RRID:MMRRC_045726-MU
Other Names:
R7648 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Uso1
Name: USO1 vesicle docking factor
Synonyms: transcytosis associated protein p115, TAP, Vdp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56041
Homologene: 2754

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ClinGen
Cnnm2
Name: cyclin M2
Synonyms: Acdp2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 94219
VEGA: 19
HGNC: HGNC:103
Homologene: 9761

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
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IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Pcm1
Name: pericentriolar material 1
Synonyms: 9430077F19Rik, 2600002H09Rik, C030044G17Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18536
HGNC: HGNC:8727
Homologene: 4518

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Cdc42bpb
Name: CDC42 binding protein kinase beta
Synonyms: DMPK-like
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217866
VEGA: 12
HGNC: HGNC:1738
Homologene: 55945

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
2610021A01Rik
Name: RIKEN cDNA 2610021A01 gene
Type: Gene
Species: Mouse
Chromosome: 7
Nup98
Name: nucleoporin 98
Synonyms: Nup96
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269966
HGNC: HGNC:8068
Homologene: 35472

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 37,431,321 bp
  • C to A, chromosome 1 at 52,196,780 bp
  • A to G, chromosome 1 at 65,073,325 bp
  • G to A, chromosome 1 at 90,933,526 bp
  • T to A, chromosome 1 at 173,857,395 bp
  • A to G, chromosome 1 at 179,709,424 bp
  • G to T, chromosome 2 at 27,112,740 bp
  • C to T, chromosome 3 at 82,108,707 bp
  • G to A, chromosome 3 at 95,750,982 bp
  • T to C, chromosome 3 at 95,898,195 bp
  • T to A, chromosome 4 at 96,499,603 bp
  • T to C, chromosome 4 at 139,965,939 bp
  • A to T, chromosome 5 at 34,431,182 bp
  • T to C, chromosome 5 at 72,523,536 bp
  • A to T, chromosome 5 at 88,504,157 bp
  • T to A, chromosome 5 at 92,194,002 bp
  • A to G, chromosome 5 at 108,644,566 bp
  • A to G, chromosome 5 at 111,183,392 bp
  • T to C, chromosome 5 at 121,254,371 bp
  • G to T, chromosome 5 at 121,796,377 bp
  • A to G, chromosome 6 at 86,628,870 bp
  • T to C, chromosome 7 at 19,619,842 bp
  • G to T, chromosome 7 at 19,852,278 bp
  • T to C, chromosome 7 at 41,612,462 bp
  • A to T, chromosome 7 at 47,426,663 bp
  • T to G, chromosome 7 at 102,124,197 bp
  • T to C, chromosome 8 at 3,610,638 bp
  • A to G, chromosome 8 at 41,275,699 bp
  • A to G, chromosome 8 at 70,297,955 bp
  • A to C, chromosome 9 at 37,992,560 bp
  • A to G, chromosome 9 at 103,227,969 bp
  • A to G, chromosome 9 at 111,391,685 bp
  • T to C, chromosome 9 at 123,856,592 bp
  • T to A, chromosome 11 at 36,106,736 bp
  • G to A, chromosome 11 at 51,234,786 bp
  • T to A, chromosome 12 at 5,027,182 bp
  • T to A, chromosome 12 at 79,055,131 bp
  • T to C, chromosome 12 at 111,377,153 bp
  • C to A, chromosome 13 at 3,649,332 bp
  • A to G, chromosome 13 at 70,589,797 bp
  • A to T, chromosome 13 at 92,274,028 bp
  • G to A, chromosome 14 at 14,773,348 bp
  • T to C, chromosome 15 at 76,110,671 bp
  • C to T, chromosome 15 at 89,421,367 bp
  • T to C, chromosome 15 at 102,089,000 bp
  • G to C, chromosome 16 at 34,879,524 bp
  • A to T, chromosome 16 at 37,563,758 bp
  • A to G, chromosome 16 at 43,989,869 bp
  • A to T, chromosome 17 at 24,445,448 bp
  • A to T, chromosome 17 at 47,674,828 bp
  • A to T, chromosome 18 at 75,637,142 bp
  • A to G, chromosome 19 at 31,083,592 bp
  • A to G, chromosome 19 at 39,803,845 bp
  • A to G, chromosome 19 at 46,877,900 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7648 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045726-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.