Strain Name:
B6J.Cg-Bsgtm1.1Riki Lyz2tm1(cre)Ifo/Mmmh
Stock Number:
046273-MU
Citation ID:
RRID:MMRRC_046273-MU
Other Names:
bsgflox/flox-lyz2-Cre

Strain Information

Bsgtm1.1Riki
Name: basigin; targeted mutation 1.1, Yasuko Rikihisa
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Conditional
Bsg
Name: basigin
Synonyms: neurothelin, CD147, 5A11/Basigin, EMMPRIN, HT-7, gp 42
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Conditional
NCBI: 12215
HGNC: HGNC:1116
Homologene: 1308
Lyz2tm1(cre)Ifo
Name: lysozyme 2; targeted mutation 1, Irmgard Forster
Synonyms: Lys-cre, Lystm1(cre)Ifo, LysM-Cre, Lyz2Cre, LysM Cre, LCre, LyzCre, Lyz-cre, lysM/cre, LC, LysMcre, LysCre, LysMCre, Lyz2-Cre, (LyzM)-Cre, LysMCre
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knock-In
Lyz2
Name: lysozyme 2
Synonyms: Lzp, Lzm, Lys, Lzm-s1, Lyzs, Lysm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knock-In
NCBI: 17105
VEGA: 10
HGNC: HGNC:6740
Homologene: 121490
Genetic Alterations

Tissue-specific knockout of Bsg exons 2-8 through cross of the floxed strain with B6.129P2-Lyz2tm1(cre)Ifo/J (RRID:IMSR_JAX:004781). A roxed neomycin-cassette in the Bsg allele was self-deleted in vitro.

ES Cell Line
  • Bsg allele: Cyagen in-house ESC line derived from C57BL/6NCrl
  • Lyz2 allele: E14.1 derived from 129P2/OlaHsd
Phenotype
None/Normal/Wild-type
Strain Development

BAC clones RP23-228L6 and RP23-94I18 were purchased from the BACPAC Resources Center (BPRC) of the Children's Hospital Oakland Research Institute (CHORI). Construction of a targeting vector was outsourced to Cyagen (conditional TurboKnockout(R)), which was electroporated into C57BL/6-derived ES cells (Cyagen, MUBES-01001) following drug selection (Rox'ed neomycin cassette was self-deleted), long-range PCR screening, and verification by Southern blot. Chimeras were bred to C57BL/6 and sib-mated to homozygosity (F3). Tissue-specific deletion was accomplished by crossing homozygotes with B6.129P2-Lyz2tm1(cre)Ifo/J (RRID:IMSR_JAX:004781) to produce males and females. Subsequent backcrossing to C57BL/6J until N10.

Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cell Biology
  • Immunology and Inflammation
  • Research Tools
Donor
Yasuko Rikihisa, Ph.D., The Ohio State University.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross & sib-mating
Generation
N10 (C57BL/6J), F10
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
046273-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.