Tissue-specific knockout of Bsg exons 2-8 through cross of the floxed strain with B6.129P2-Lyz2^tm1(cre)Ifo/J (RRID:IMSR_JAX:004781). A roxed neomycin-cassette in the Bsg allele was self-deleted in vitro.

• Bsg allele: Cyagen in-house ESC line derived from C57BL/6NCrl
• Lyz2 allele: E14.1 derived from 129P2/OlaHsd

BAC clones RP23-228L6 and RP23-94I18 were purchased from the BACPAC Resources Center (BPRC) of the Children's Hospital Oakland Research Institute (CHORI). Construction of a targeting vector was outsourced to Cyagen (conditional TurboKnockout^(R)), which was electroporated into C57BL/6-derived ES cells (Cyagen, MUBES-01001) following drug selection (Rox'ed neomycin cassette was self-deleted), long-range PCR screening, and verification by Southern blot. Chimeras were bred to C57BL/6 and sib-mated to homozygosity (F3). Tissue-specific deletion was accomplished by crossing homozygotes with B6.129P2-Lyz2^tm1(cre)Ifo/J (RRID:IMSR_JAX:004781) to produce males and females. Subsequent backcrossing to C57BL/6J until N10.

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Colony Surveillance Program and Current Health Reports

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.