Strain Detail Sheet
Tissue-specific knockout of Bsg exons 2-8 through cross of the floxed strain with B6.129P2-Lyz2tm1(cre)Ifo/J (RRID:IMSR_JAX:004781). A roxed neomycin-cassette in the Bsg allele was self-deleted in vitro.
- Bsg allele: Cyagen in-house ESC line derived from C57BL/6NCrl
- Lyz2 allele: E14.1 derived from 129P2/OlaHsd
BAC clones RP23-228L6 and RP23-94I18 were purchased from the BACPAC Resources Center (BPRC) of the Children's Hospital Oakland Research Institute (CHORI). Construction of a targeting vector was outsourced to Cyagen (conditional TurboKnockout(R)), which was electroporated into C57BL/6-derived ES cells (Cyagen, MUBES-01001) following drug selection (Rox'ed neomycin cassette was self-deleted), long-range PCR screening, and verification by Southern blot. Chimeras were bred to C57BL/6 and sib-mated to homozygosity (F3). Tissue-specific deletion was accomplished by crossing homozygotes with B6.129P2-Lyz2tm1(cre)Ifo/J (RRID:IMSR_JAX:004781) to produce males and females. Subsequent backcrossing to C57BL/6J until N10.
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Colony Surveillance Program and Current Health Reports
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .| Viability and Fertility: | Female | Male | Comments |
|---|---|---|---|
| Homozygotes are viable: | Yes | Yes | |
| Homozygotes are fertile: | Yes | Yes | |
| Heterozygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | Undetermined | Undetermined |
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
The donor or their institution limits the distribution to non-profit institutions only.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
|---|---|---|---|---|
| 046273-MU-RESUS | Litter recovered from cryo-archive | $2,624.00 / Non-Profit | Litter | Recovered litter4; additional fees for any special requests. |
| Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information. | ||||
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.