Loading Mouse GIF
Loading...

Strain Name:
B6J.B6N(FVB)-Atm1Brd Mirc11tm1.1Wtsi/AnseMmucd
Stock Number:
046279-UCD
Citation ID:
RRID:MMRRC_046279-UCD
Other Names:
Mirc11tm1Wtsi ; Sanger MirKO ES cell line Mir23a-Mir24-2 cluster 8N2, 036362-UCD, Mutant Mouse Regional Resource Centers

Strain Information

Mirc11tm1Wtsi
Name: microRNA cluster 11, including Mir23a through Mir24-2; targeted mutation 1, Wellcome Trust Sanger Institute
Synonyms: Mir23atm1(mirKO)Wtsi, Mirc11tm1(mirKO)Wtsi, Mir23a-Mir24-2 cluster 8N2
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
Mirc11
Name: microRNA cluster 11, including Mir23a through Mir24-2
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
Mir23a
Name: microRNA 23a
Synonyms: mmu-mir-23a, Mirn23a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
NCBI: 387216
VEGA: 8
Mir27a
Name: microRNA 27a
Synonyms: mmu-mir-27a, Mirn27a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
NCBI: 387220
VEGA: 8
Mir24-2
Name: microRNA 24-2
Synonyms: mir 24-2, mmu-mir-24-2, Mirn24-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
NCBI: 723960
VEGA: 8
Genetic Alterations
Knockout of the miRNA cluster containing miR-23a, miR-27a and miR-24-2. This mouse was generated from ES cells banked at the MMRRC (MMRRC:36362).
Genotype Determination
ES Cell Line
JM8A3
Phenotype
None/Normal/Wild-type
MeSH Terms
  • Animals
  • Asthma/immunology
  • Base Sequence
  • Cell Differentiation/genetics
  • Cell Differentiation/immunology
  • Cells, Cultured
  • Disease Models, Animal
  • Female
  • Inflammation/immunology
  • Interleukin-4/biosynthesis
  • Interleukin-4/immunology
  • Lymphocyte Activation/immunology
  • Male
  • Mice
  • Mice, Knockout
  • MicroRNAs/genetics
  • Multigene Family/genetics
  • Sequence Analysis, RNA
  • Th2 Cells/cytology
  • Th2 Cells/immunology
Strain Development
ES cells MMRRC:36362 were used for microinjection into blastocysts. Chimeras were crossed to B6N.FVB-Tmem163Tg(ACTB-cre)2Mrt/CjDswJ mice (RRID:IMSR_JAX:019099) to delete the selection cassette. Subsequent C57L/6J backrossing to N5. Lines were maintained through intercrossing of siblings.
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Immunology and Inflammation
Donor
K Mark Ansel, University of California, San Francisco.
Primary Reference

Pua HH, Steiner DF, Patel S, Gonzalez JR, Ortiz-Carpena JF, Kageyama R, Chiou NT, Gallman A, de Kouchkovsky D, Jeker LT, McManus MT, Erle DJ, Ansel KM.MicroRNAs 24 and 27 Suppress Allergic Inflammation and Target a Network ofRegulators of T Helper 2 Cell-Associated Cytokine Production. Immunity. 2016 Apr 19;44(4):821-32. doi: 10.1016/j.immuni.2016.01.003. Epub 2016 Feb 2. (Medline PMID: 26850657)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
N5 (C57BL/6J), F3
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
046279-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
046279-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.