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Strain Detail Sheet

Strain Name:
B6J.B6N-Mir205hgtm1Oers/Mmnc
Stock Number:
065422-UNC
Citation ID:
RRID:MMRRC_065422-UNC
Other Names:
MIR205HGfl

Strain Information

Gene Details

Mir205hgtm1Oers
Name: Mir205 host gene; targeted mutation 1, Nicolai S van Oers
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Mir205hg
Name: Mir205 host gene
Synonyms: 4631405K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
NCBI: 70800

Additional Resources
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Genetic Alterations
One LoxP site was introduced at the 5' end of the long noncoding RNA, Mir205hg, and a second LoxP site prior to the segment encoding the microRNA, miR-205 (MGI:2676880). This design ensured that the majority of the long noncoding RNA, a 2.5-Mb segment, is eliminated following Cre recombination. The sequence comprising the microRNA remained intact. This line can be used with different Cre transgenics to create tissue-specific knockouts.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    JM8 derived from C57BL/6N

    Strain Description

    Phenotype

    The floxed mice have a wild-type phenotype.

    Once crossed with a Cre-expressing line (e.g., CAG-Cre such as JAX Strain #004682), the mice displayed growth delay in the donor's laboratory. More specifically, they had a deficiency of a long noncoding RNA, Mir205hg. This led to reduced production of growth hormone and prolactin in young mice, causing a temporal growth impairment. The mechanism involved the long noncoding RNA interactions with a transcription factor, Pit1, and was independent of the embedded microRNA encoded by Mir205 (NR_029592.1).

    Strain GQC Summary
    The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
    Suggested Control Mice
    Littermates of all relevant genotypes.

    Research Applications

    • Cancer
    • Developmental Biology
    • Endocrine Deficiency
    • Immunology and Inflammation

    Strain Origin

    Donor
    Nicolai S.C. van Oers, Ph.D., University of Texas Southwestern Medical Center.
    Primary Reference

    Du Q, Hoover AR, Dozmorov I, Raj P, Khan S, Molina E, Chang TC, de la MorenaMT, Cleaver OB, Mendell JT, van Oers NSC. MIR205HG Is a Long Noncoding RNA thatRegulates Growth Hormone and Prolactin Production in the Anterior Pituitary. Dev Cell. 2019 May 20;49(4):618-631.e5. doi: 10.1016/j.devcel.2019.03.012. Epub 2019 Apr 11. (Medline PMID: 30982661)

    Strain Development
    Targeting vector based on pGKneoF2L2dta (Addgene plasmid #13445). The right arm contained 2.3 kb of the regulatory region of Mir205hg. The left arm was 4.1 kB and contained loxP sites inserted 100 bp upstream of Mir205hg. Electroporation of linearized target vector into JM8 ES cells (C57BL/6N) under G418 selection, clonal expansion, and screening via Southern blot for correct targeting into the Mir205hg locus. One ES cell clone, 4H11, was injected into albino B6 blastocysts, and 7 distinct chimeric male mice were recovered. Chimerism ranged from 20-90%. Mice were crossed onto C57BL/J to produce targeted allele animals. The animals were backcrossed to C57BL/6J for 11 generations.

    While the mice described here are still floxed, the donor already tested the elimination of the foxed allele through crosses to CAG-Cre (e.g., JAX Strain #006084 - embryonic targeting; see Phenotype description above). Other crosses are suggested in the context, such as Foxg1-Cre (e.g., JAX Strain #004337) or Foxn1-Cre (e.g., JAX Strain #018448).


    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Sib-mating
    Generation
    N11 (C57BL/6J), F12
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 4 to 6 months Undetermined
    Average litter size
    7 to 9
    Recommended wean age
    4 Weeks
    Average Pups Weaned
    7 to 9

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    065422-UNC-RESUS Litter recovered from cryo-archive $3,242.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.