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The tTA coding sequence was released as an EcoRI/BamHI-blunted fragment from pMSCV-tTA-IRES-GFP. The tTA sequence was directly inserted into the blunted EcoRI site of the MMTV-SV40-BSSK vector (provided by Dr. Muller, McGill Univ.) that had been modified at the SalI site as SalI-SpeI-NotI-SpeI-SalI sequence. The mouse mammary gland tumor virus (MMTV) promoter drives mammary gland-specific expression of the tetracycline-controlled transactivator (tTA). In 2020-2021, analysis performed by The Jackson Laboratory discovered that at least three tandem repeats/copies of the Tg(MMTV-tTA)25754Kuw transgene (hemizygous) integrated on chromosome 8 (chr8:12,485,790-12,564,686; mouse mm10), approximately 10kb upstream of the Spaca7 locus. Currently, there are no genes annotated in the integration region. This analysis also identified the transgene includes an SV40 NLS and polyA signal.
Sakamoto K, Schmidt JW, Wagner KU. Generation of a novel MMTV-tTA transgenicmouse strain for the targeted expression of genes in the embryonic and postnatal mammary gland. PLoS One. 2012;7(8):e43778. doi: 10.1371/journal.pone.0043778.Epub 2012 Aug 31. PubMed PMID: 22952764; PubMed Central (Medline PMCID: PMC3432039)
Zhang Q, Sakamoto K, Wagner KU. D-type Cyclins are important downstreameffectors of cytokine signaling that regulate the proliferation of normal andneoplastic mammary epithelial cells. Mol Cell Endocrinol. 2014 Jan25;382(1):583-592. doi: 10.1016/j.mce.2013.03.016. Epub 2013 Apr 4. Review.PubMed PMID: 23562856; PubMed Central (Medline PMCID: PMC3740091)
Zboray K, Mohrherr J, Stiedl P, Pranz K, Wandruszka L, Grabner B, Eferl R,Moriggl R, Stoiber D, Sakamoto K, Wagner KU, Popper H, Casanova E, Moll HP. AKT3 drives adenoid cystic carcinoma development in salivary glands. Cancer Med. 2018 Feb;7(2):445-453. doi: 10.1002/cam4.1293. Epub 2017 Dec 28. PubMed PMID:29282901; PubMed Central (Medline PMCID: PMC5806106)
Schmidt JW, Wehde BL, Sakamoto K, Triplett AA, Anderson SM, Tsichlis PN, LeoneG, Wagner KU. Stat5 regulates the phosphatidylinositol 3-kinase/Akt1 pathwayduring mammary gland development and tumorigenesis. Mol Cell Biol. 2014Apr;34(7):1363-77. doi: 10.1128/MCB.01220-13. Epub 2014 Jan 27. PubMed PMID:24469394; PubMed Central (Medline PMCID: PMC3993568)
Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.
The donor or their institution limits the distribution to non-profit institutions only.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.