Strain Name:
FVB/N-Tg(MMTV-tTA)25754Kuw/Mmjax
Stock Number:
066724-JAX
Citation ID:
RRID:MMRRC_066724-JAX
Other Names:
Tg(MMTV-tTA)25754Kuw

Gene Information

Tg(MMTV-tTA)25754Kuw
Name: transgene insertion 25754, Kay-Uwe Wagner
Synonyms: MMTV-tTA
Type: Allele
Species: Multi-species
Alteration at locus: Transgenic
MMTV
Name: Mouse mammary tumor virus promoter
Type: DNA Segment
Species: Virus
Alteration at locus: Transgenic
tTA
Name: tetracycline-controlled transactivator protein
Type: Gene
Species: Multi-species
Alteration at locus: Transgenic
Genetic Alterations

The tTA coding sequence was released as an EcoRI/BamHI-blunted fragment from pMSCV-tTA-IRES-GFP. The tTA sequence was directly inserted into the blunted EcoRI site of the MMTV-SV40-BSSK vector (provided by Dr. Muller, McGill Univ.) that had been modified at the SalI site as SalI-SpeI-NotI-SpeI-SalI sequence. The mouse mammary gland tumor virus (MMTV) promoter drives mammary gland-specific expression of the tetracycline-controlled transactivator (tTA).

In 2020-2021, analysis performed by The Jackson Laboratory discovered that at least three tandem repeats/copies of the Tg(MMTV-tTA)25754Kuw transgene (hemizygous) integrated on chromosome 8 (chr8:12,485,790-12,564,686; mouse mm10), approximately 10kb upstream of the Spaca7 locus. Currently, there are no genes annotated in the integration region. This analysis also identified the transgene includes an SV40 NLS and polyA signal.

Genotype Determination
Phenotype
None/Normal/Wild-type
MeSH Terms
  • Animals
  • Cell Line
  • Embryo, Mammalian/metabolism
  • Epithelial Cells/metabolism
  • Female
  • Gene Deletion
  • Gene Expression
  • Genes, erbB-2/genetics
  • Integrases/genetics
  • Ligands
  • Mammary Glands, Animal/metabolism
  • Mammary Neoplasms, Experimental/genetics
  • Mammary Tumor Virus, Mouse/genetics
  • Mice
  • Mice, Transgenic/genetics
  • Terminal Repeat Sequences/genetics
  • Tetracycline/pharmacology
  • Trans-Activators/genetics
  • Transcriptional Activation/drug effects
  • Breast Neoplasms/metabolism
  • Breast Neoplasms/pathology
  • Cell Proliferation
  • Cyclins/metabolism
  • Cytokines/metabolism
  • Epithelial Cells/pathology
  • Humans
  • Signal Transduction
Strain Development
The MMTV-tTA transgene was generated by inserting the tTA cDNA into the blunted EcoRI site of the MMTV-SV40-BSSK vector (provided by Dr. Muller, McGill). The initial injection was into FVB/NJ zygotes. Litters were generated and two founder lines were obtained (25754 and 25755) with identical expression patterns. Said founder lines were maintained by crossing with FVB/N WT mice for many years.

The MMTV-tTA transgene was detected by PCR using primers corresponding to the MMTV LTR and the tTA coding sequence (2127; 5’- AGT GAT AGA GCT CTT GCC TAG C-3’ and 599; 5’-GCC AAT ACA GTG TAG GCT GC-3’). Alternatively, primer sets were used detecting the 3’ end of the tTA sequence and the SV40 intron/polyA sequence (421; 5’- CGC TAG ACG ATT TCG ATC TGG-3’ and 2128; 5’- CTC CCA TTC ATC AGT TCC ATA GG-3’).
Suggested Control Mice
FVB/N
  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
  • Research Tools
Donor
Kay-Uwe Wagner, Ph.D., Karmanos Cancer Institute.
Primary Reference

Sakamoto K, Schmidt JW, Wagner KU. Generation of a novel MMTV-tTA transgenicmouse strain for the targeted expression of genes in the embryonic and postnatal mammary gland. PLoS One. 2012;7(8):e43778. doi: 10.1371/journal.pone.0043778.Epub 2012 Aug 31. PubMed PMID: 22952764; PubMed Central (Medline PMCID: PMC3432039)

Zhang Q, Sakamoto K, Wagner KU. D-type Cyclins are important downstreameffectors of cytokine signaling that regulate the proliferation of normal andneoplastic mammary epithelial cells. Mol Cell Endocrinol. 2014 Jan25;382(1):583-592. doi: 10.1016/j.mce.2013.03.016. Epub 2013 Apr 4. Review.PubMed PMID: 23562856; PubMed Central (Medline PMCID: PMC3740091)

Zboray K, Mohrherr J, Stiedl P, Pranz K, Wandruszka L, Grabner B, Eferl R,Moriggl R, Stoiber D, Sakamoto K, Wagner KU, Popper H, Casanova E, Moll HP. AKT3 drives adenoid cystic carcinoma development in salivary glands. Cancer Med. 2018 Feb;7(2):445-453. doi: 10.1002/cam4.1293. Epub 2017 Dec 28. PubMed PMID:29282901; PubMed Central (Medline PMCID: PMC5806106)

Schmidt JW, Wehde BL, Sakamoto K, Triplett AA, Anderson SM, Tsichlis PN, LeoneG, Wagner KU. Stat5 regulates the phosphatidylinositol 3-kinase/Akt1 pathwayduring mammary gland development and tumorigenesis. Mol Cell Biol. 2014Apr;34(7):1363-77. doi: 10.1128/MCB.01220-13. Epub 2014 Jan 27. PubMed PMID:24469394; PubMed Central (Medline PMCID: PMC3993568)

Colony and Husbandry Information

For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
White
Eye
Pink
MMRRC Breeding System
Random intra-strain mating
Generation
N15+ (FVB/N)
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Average litter size
10 to 14
Recommended wean age
3 Weeks
Average Pups Weaned
10 to 14

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
066724-JAX-HET-F
066724-JAX-HET-M
066724-JAX-WT-F
066724-JAX-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
$218.00 / Non-Profit Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.