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Availability & Fees Order this Strain
Genotyping Protocol
A reporter marker cassette was inserted in antisense orientation downstream of Retnla exon 4, such that cre-mediated deletion of the targeted exon 2 to exon 4 will flip the reporter gene (tdTomato) into sense orientation leading to its expression. To enhance the stability of the reporter messenger and to increase the chance of having a bright signal, a WPRE sequence (woodchuck hepatitis virus post-transcriptional regulatory element - see PMID:12787924) was added to the construct.
Vector design: Previously isolated homology fragments and cDNA were used to generate the targeting vector (NAI1-HR targeting vector for Retnla Exons 2-4) for homologous recombination in electroporated ES cells derived from C57BL/6N (genOway, proprietary). Following neomycin selection and clonal expansion, Southern blotting confirmed correct targeting. Six ES clones were injected into blastocysts and implanted into pseudopregnant dams. Tail-snip PCR confirmed the genotype of the chimeras, whose offspring were mated with C57BL/6N (Charles River) for germline transmission. Three founders were identified and bred with C57BL/6J (RRID:IMSR_JAX:000664) mice for two generations after the selection cassette had been removed with a C57BL/6N Flp recombinase strain (genOway, proprietary).
Note: This line is available as a constitutive knockout in MMRRC:67014.
Colony Surveillance Program and Current Health Reports
Black
6-9
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.