Strain Detail Sheet

Strain Name:
B6N(Cg)-Dsppem2Jpsi/Mmnc
Stock Number:
067180-UNC
Citation ID:
RRID:MMRRC_067180-UNC
Other Names:
Dspp-2 frameshift, B6N;SJL-Dsppem2Jpsi/Mmnc

Strain Information

Gene Details

Dsppem2Jpsi
Name: dentin sialophosphoprotein; endonuclease-mediated mutation 2, James Simmer
Synonyms: Dspp-DPP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: CRISPR
Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: CRISPR
NCBI: 666279
HGNC: HGNC:3054
Genome Browser [Click image to go to Jbrowse]



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Genetic Alterations
The Dspp gene encodes the dentin sialophosphoprotein. A Flag tag was inserted at the C-terminus of DSP and a single nucleotide (T) insertion in the coding region of DPP.

HGVS nomenclature (Dspp variant):

  • Genbank RefSeq - mRNA: NM_010080.3
  • Genbank RefSeq, protein: NP_034210.2
  • Variant, nucleic acid level: c.1380dup
  • Variant, amino acid level, predicted: p.Asn461*
    Check this variant: LUMC Mutalyzer

Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Not specified

    Strain Description

    Phenotype
    Upon secretion by the odontoblast, dentin sialophosphoprotein is cleaved to form dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). This mouse model allows for the assessment of associated protein production, secretion, and characterization of dentin malformation in the absence of functional DPP.

    In terms of growth and development, reproduction, and life span, there are no notable deviations from the wild-type. The formation and mineralization of tooth dentin differ slightly from that of the age-comparable wild-type mice.
    Strain Development
    Using CRISPR/Cas9 gene-editing technology on a B6NSJLF1 background, above-noted genetic alterations were introduced. F1 animals were mated to C57BL/6N for 2 generations before sib-pair mating began.
    Suggested Control Mice
    Wild-type littermate

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

    GQC analysis was done on two samples RR9773 (XY) and PC9140 (XX), at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and RR9773 is the representative sample selected for the report. The primary background is C57BL/6NCrl. This is a line that was generated by CRISPR genome editing in a C57BL/6N X SJL F1 background and no SJL contribution is detected by MiniMUGA. The mitochondrial genome is consistent with at least one backcross to a female from the host background. The Y chromosome is consistent with at least one backcross to a male from the host background. The region containing the allele of interest has the expected C57BL/6NCrl background . Both samples are homozygous for the C57BL/6NCrl background at the locus and none of the constructs detectable by MiniMUGA are present.

    The genome of the MMRRC strain: 67180 is 100 % inbred at the strain level. The level of congenicity estimated by MiniMUGA is greater than what would be predicted from the number of backcross generations reported in the SDS (2).

    Special Considerations: Based on this report, a request has been made to update the strain name from B6N;SJL-Dsppem2Jpsi/Mmnc to B6N(Cg)-Dsppem2Jpsi/Mmnc (1/3/2023)
    MiniMUGA Sample Report
    MiniMUGA Sample Report Link

    Research Applications

    • Developmental Biology

    Strain Origin

    Donor
    James Simmer, Ph.D., University of Michigan.
    Jan Ching Chun Hu, Ph.D., University of Michigan.
    Primary Reference
    In preparation, submitted, or in press

    Colony and Husbandry Information

    Special Considerations

    Because of tooth defects, the null mice are maintained on hard chow and diet gel. "Love Mash" is an acceptable alternative for mice with tooth defects.

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backcross and sib-mating
    Generation
    N2 (C57BL/6NCrl), F5+
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 4 to 6 months 4 to 6 months
    Bred to Homozygosity
    Yes
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    067180-UNC-SPERM Cryo-preserved spermatozoa $564.00 / $937.00
    Non-Profit / For-Profit    		 Aliquot Approximate quantity3
    067180-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.