Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8063Btlr/Mmmh
Stock Number:
067499-MU
Citation ID:
RRID:MMRRC_067499-MU
Other Names:
R8063 (G1)
Major Collection:

Strain Information

Lasp1
Name: LIM and SH3 protein 1
Synonyms: SH3P6, Def-4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16796
HGNC: HGNC:6513
Homologene: 4480
Cep70
Name: centrosomal protein 70
Synonyms: 6720484E09Rik, C030018L16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68121
Homologene: 11387
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Usp30
Name: ubiquitin specific peptidase 30
Synonyms: 6330590F17Rik, D5Ertd483e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100756
Homologene: 32789
Sin3b
Name: transcriptional regulator, SIN3B (yeast)
Synonyms: 2810430C10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20467
Homologene: 81810
Asxl2
Name: ASXL transcriptional regulator 2
Synonyms: 4930556B16Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 75302
Homologene: 10102
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 40,487,038 bp
  • T to A, chromosome 1 at 53,066,448 bp
  • T to C, chromosome 1 at 120,063,275 bp
  • C to T, chromosome 1 at 158,936,556 bp
  • A to G, chromosome 1 at 167,466,521 bp
  • G to A, chromosome 2 at 118,410,901 bp
  • A to G, chromosome 2 at 146,443,855 bp
  • A to T, chromosome 2 at 155,702,456 bp
  • A to G, chromosome 4 at 70,488,258 bp
  • G to T, chromosome 4 at 111,459,834 bp
  • C to T, chromosome 4 at 128,586,235 bp
  • T to C, chromosome 4 at 145,114,757 bp
  • T to C, chromosome 5 at 21,648,770 bp
  • T to C, chromosome 5 at 24,321,672 bp
  • A to T, chromosome 5 at 86,152,685 bp
  • A to G, chromosome 5 at 114,100,463 bp
  • A to T, chromosome 6 at 35,068,643 bp
  • T to A, chromosome 6 at 40,329,556 bp
  • T to G, chromosome 6 at 56,985,598 bp
  • T to C, chromosome 6 at 83,402,741 bp
  • T to A, chromosome 6 at 124,024,955 bp
  • A to G, chromosome 7 at 15,979,044 bp
  • A to G, chromosome 8 at 71,998,231 bp
  • G to A, chromosome 8 at 72,725,541 bp
  • A to T, chromosome 9 at 39,623,527 bp
  • C to T, chromosome 9 at 66,097,394 bp
  • T to A, chromosome 9 at 99,296,122 bp
  • A to G, chromosome 10 at 78,714,031 bp
  • A to T, chromosome 11 at 50,308,346 bp
  • A to G, chromosome 11 at 82,889,771 bp
  • T to C, chromosome 11 at 97,685,884 bp
  • T to C, chromosome 11 at 97,834,131 bp
  • G to C, chromosome 11 at 100,265,039 bp
  • C to A, chromosome 11 at 101,630,868 bp
  • T to C, chromosome 11 at 103,542,261 bp
  • T to A, chromosome 11 at 105,971,364 bp
  • A to G, chromosome 12 at 3,500,768 bp
  • T to C, chromosome 12 at 114,723,785 bp
  • T to C, chromosome 13 at 23,875,541 bp
  • G to A, chromosome 13 at 36,204,897 bp
  • A to T, chromosome 13 at 43,370,847 bp
  • A to G, chromosome 14 at 56,366,088 bp
  • A to T, chromosome 14 at 78,278,658 bp
  • C to T, chromosome 15 at 6,772,154 bp
  • T to A, chromosome 15 at 28,230,583 bp
  • T to A, chromosome 16 at 36,041,788 bp
  • T to A, chromosome 16 at 56,261,456 bp
  • C to A, chromosome 17 at 17,088,361 bp
  • C to T, chromosome 18 at 20,032,274 bp
  • A to G, chromosome 18 at 60,838,762 bp
  • G to A, chromosome 18 at 65,350,346 bp
  • T to C, chromosome 19 at 3,911,019 bp
  • T to C, chromosome 19 at 10,964,772 bp
  • T to A, chromosome 19 at 50,143,977 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8063 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067499-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.