Strain Name:
C57BL/6J-MtgxR8249Btlr/Mmmh
Stock Number:
067649-MU
Citation ID:
RRID:MMRRC_067649-MU
Other Names:
R8249 (G1)
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: c-KIT, belly-spot, CD117, SCO5, Tr-kit, SOW3, Steel Factor Receptor, Gsfsco1, Gsfsow3, Dominant white spotting, Gsfsco5, SCO1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Pi4k2a
Name: phosphatidylinositol 4-kinase type 2 alpha
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 84095
VEGA: 19
Homologene: 101681
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4833417A11Rik, 4832428G11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Adck2
Name: aarF domain containing kinase 2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 57869
Homologene: 49103
Tti1
Name: TELO2 interacting protein 1
Synonyms: 2610036D13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75425
Homologene: 40969
Arhgap12
Name: Rho GTPase activating protein 12
Synonyms: 2810011M08Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 75415
Homologene: 23089
Gcfc2
Name: GC-rich sequence DNA binding factor 2
Synonyms: AW146020
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330361
HGNC: HGNC:1317
Homologene: 2411
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Grin2c
Name: glutamate receptor, ionotropic, NMDA2C (epsilon 3)
Synonyms: NR2C, NMDAR2C, GluRepsilon3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14813
HGNC: HGNC:4587
Homologene: 647
Tceanc2
Name: transcription elongation factor A (SII) N-terminal and central domain containing 2
Synonyms: Tdeanc2, 6330404A07Rik, 2210012G02Rik, 2210010B22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66526
Homologene: 11984
Gpr165
Name: G protein-coupled receptor 165
Synonyms: 6530406P05Rik
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 76206
Homologene: 85287
AW554918
Name: expressed sequence AW554918
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225289
Homologene: 52652
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: b2b1554Clo, CD91, A2mr
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Mroh7
Name: maestro heat-like repeat family member 7
Synonyms: Gm1027, Heatr8, LOC381538
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381538
Homologene: 19633
Hmcn1
Name: hemicentin 1
Synonyms: EG545370, LOC240793
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 545370
Homologene: 23741
Unc80
Name: unc-80, NALCN activator
Synonyms: C030018G13Rik, C230061B10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329178
Homologene: 122243
Nlrp9c
Name: NLR family, pyrin domain containing 9C
Synonyms: Nalp-zeta, Nalp9c
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330490
Homologene: 116072
Camk2d
Name: calcium/calmodulin-dependent protein kinase II, delta
Synonyms: 2810011D23Rik, 8030469K03Rik, CaMK II
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 108058
HGNC: HGNC:1462
Homologene: 55561
Pwwp2b
Name: PWWP domain containing 2B
Synonyms: D930023J19Rik, D7Ertd517e, Pwwp2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101631
Homologene: 19056
Dnm3
Name: dynamin 3
Synonyms: 9630020E24Rik, B230343F03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 103967
Homologene: 22906
Serpina1f
Name: serine (or cysteine) peptidase inhibitor, clade A, member 1F
Synonyms: 0610012A11Rik, epserin, Serpina2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68348
HGNC: HGNC:8985
Homologene: 87037
Gm136
Name: predicted gene 136
Synonyms: LOC214568
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 214568
Homologene: 79759
Fkbp6
Name: FK506 binding protein 6
Synonyms: D5Ertd724e, 1700008G22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 94244
HGNC: HGNC:3722
Homologene: 2672
Sdk1
Name: sidekick cell adhesion molecule 1
Synonyms: 6720466O15Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 330222
Homologene: 27395
Agbl2
Name: ATP/GTP binding protein-like 2
Synonyms: A430081C19Rik, Ccp2, Ccp2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 271813
Homologene: 11715
Bscl2
Name: BSCL2 lipid droplet biogenesis associated, seipin
Synonyms: seipin, Gng3lg
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14705
Homologene: 32032
Hars2
Name: histidyl-tRNA synthetase 2
Synonyms: Harsl, HO3, HARSR, 4631412B19Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 70791
HGNC: HGNC:4817
Homologene: 40819
Mtrf1
Name: mitochondrial translational release factor 1
Synonyms: A830062K05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211253
VEGA: 14
HGNC: HGNC:7469
Homologene: 20903
Cnga1
Name: cyclic nucleotide gated channel alpha 1
Synonyms: Cncg
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12788
HGNC: HGNC:2148
Homologene: 55432
Mterf1a
Name: mitochondrial transcription termination factor 1a
Synonyms: 9230106K09Rik, Mterf, Mterf1, 4931431L11Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 545725
Homologene: 5073
Musk
Name: muscle, skeletal, receptor tyrosine kinase
Synonyms: Nsk1, Nsk2, Nsk3, MDK4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18198
HGNC: HGNC:7525
Homologene: 4084
Hycc2
Name: hyccin PI4KA lipid kinase complex subunit 2
Synonyms: Fam126b, D1Ertd53e
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 213056
Homologene: 18184
H2-M11
Name: histocompatibility 2, M region locus 11
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224754
Homologene: 52241
Tmem156
Name: transmembrane protein 156
Synonyms: LOC243025
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243025
Homologene: 49811
Map3k9
Name: mitogen-activated protein kinase kinase kinase 9
Synonyms: Mlk1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 338372
VEGA: 12
HGNC: HGNC:6861
Homologene: 76377
Rftn1
Name: raftlin lipid raft linker 1
Synonyms: 2310015N21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76438
Homologene: 18745
Lrfn3
Name: leucine rich repeat and fibronectin type III domain containing 3
Synonyms: A530045B06Rik, SALM4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233067
Homologene: 11558
Ifit1
Name: interferon-induced protein with tetratricopeptide repeats 1
Synonyms: ISG56, Ifi56
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15957
Homologene: 22462
Krt32
Name: keratin 32
Synonyms: mHa2, Krt1-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16670
HGNC: HGNC:6449
Homologene: 68246
Trbv16
Name: T cell receptor beta, variable 16
Synonyms: Gm16776, Tcrb-V11
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 100124680
Wnt2
Name: wingless-type MMTV integration site family, member 2
Synonyms: Mirp, Irp, Wnt-2, Int1l1, 2610510E18Rik, Wnt2a, m-irp
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22413
Homologene: 20719
Btd
Name: biotinidase
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 26363
HGNC: HGNC:1122
Homologene: 37255
Fbxo48
Name: F-box protein 48
Synonyms: A630050E13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319701
Homologene: 18541
Apoa1bp
Name:
Type: Gene
Species: Mouse
Chromosome: 3
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 58,534,637 bp
  • A to T, chromosome 1 at 66,619,491 bp
  • A to G, chromosome 1 at 71,321,812 bp
  • A to T, chromosome 1 at 150,819,366 bp
  • A to T, chromosome 1 at 162,477,743 bp
  • G to A, chromosome 2 at 90,797,564 bp
  • C to G, chromosome 2 at 158,000,715 bp
  • A to T, chromosome 3 at 88,058,388 bp
  • C to T, chromosome 3 at 126,797,729 bp
  • C to A, chromosome 4 at 34,750,955 bp
  • T to G, chromosome 4 at 58,368,926 bp
  • T to C, chromosome 4 at 106,721,212 bp
  • G to A, chromosome 4 at 107,178,993 bp
  • A to C, chromosome 5 at 3,891,550 bp
  • T to A, chromosome 5 at 65,075,626 bp
  • T to C, chromosome 5 at 72,605,394 bp
  • T to C, chromosome 5 at 75,641,408 bp
  • A to G, chromosome 5 at 135,349,952 bp
  • A to T, chromosome 5 at 142,188,015 bp
  • C to A, chromosome 6 at 18,030,285 bp
  • C to T, chromosome 6 at 39,585,733 bp
  • A to G, chromosome 6 at 41,151,998 bp
  • T to C, chromosome 6 at 81,956,951 bp
  • T to A, chromosome 7 at 26,375,353 bp
  • GC to GCCAGGGGAGGTGAGCGGTGGGTCACCTCCC, chromosome 7 at 30,359,873 bp
  • G to T, chromosome 7 at 139,254,843 bp
  • T to A, chromosome 9 at 119,617,774 bp
  • C to A, chromosome 10 at 127,605,543 bp
  • T to A, chromosome 11 at 16,953,433 bp
  • T to C, chromosome 11 at 100,086,722 bp
  • C to T, chromosome 11 at 115,253,837 bp
  • T to C, chromosome 12 at 81,780,777 bp
  • C to T, chromosome 12 at 103,693,768 bp
  • T to C, chromosome 12 at 116,000,224 bp
  • T to C, chromosome 14 at 31,665,948 bp
  • C to A, chromosome 14 at 79,401,479 bp
  • T to A, chromosome 17 at 36,549,008 bp
  • G to T, chromosome 17 at 50,047,380 bp
  • A to T, chromosome 18 at 6,027,635 bp
  • C to T, chromosome 18 at 25,339,718 bp
  • A to T, chromosome 18 at 36,788,001 bp
  • T to C, chromosome 19 at 8,846,520 bp
  • T to A, chromosome 19 at 34,640,989 bp
  • A to G, chromosome 19 at 42,115,062 bp
  • C to A, chromosome X at 96,714,017 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8249 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067649-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.