Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8492Btlr/Mmmh
Stock Number:
067934-MU
Citation ID:
RRID:MMRRC_067934-MU
Other Names:
R8492 (G1)
Major Collection:

Strain Information

Rpl13a
Name: ribosomal protein L13A
Synonyms: tum-antigen, tum-transplantation antigen P198, Tstap198-7, 1810026N22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22121
Homologene: 111053
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Prok2
Name: prokineticin 2
Synonyms: Bombina variegata 8kDa protein, Bv8, PK2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 50501
Homologene: 9268
Asah2
Name: N-acylsphingosine amidohydrolase 2
Synonyms: neutral/alkaline ceramidase
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 54447
VEGA: 19
Homologene: 10310
Triobp
Name: TRIO and F-actin binding protein
Synonyms: Mus EST 478828, Tara, EST478828
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110253
Homologene: 5104
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Tdh
Name: L-threonine dehydrogenase
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 58865
Homologene: 10987
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 44,206,393 bp
  • T to C, chromosome 1 at 59,211,344 bp
  • C to G, chromosome 1 at 85,577,214 bp
  • A to G, chromosome 1 at 189,658,729 bp
  • T to G, chromosome 2 at 15,610,123 bp
  • T to A, chromosome 2 at 37,068,683 bp
  • T to C, chromosome 2 at 52,313,212 bp
  • A to G, chromosome 2 at 58,137,857 bp
  • A to G, chromosome 2 at 69,220,242 bp
  • T to A, chromosome 2 at 103,092,521 bp
  • A to T, chromosome 2 at 146,342,604 bp
  • T to G, chromosome 3 at 32,751,228 bp
  • T to G, chromosome 3 at 96,682,213 bp
  • T to C, chromosome 4 at 129,489,652 bp
  • T to C, chromosome 4 at 147,861,424 bp
  • A to T, chromosome 5 at 65,065,095 bp
  • A to C, chromosome 5 at 93,271,454 bp
  • G to A, chromosome 5 at 135,889,368 bp
  • A to C, chromosome 5 at 137,112,929 bp
  • A to T, chromosome 6 at 38,190,590 bp
  • A to G, chromosome 6 at 90,223,412 bp
  • G to C, chromosome 6 at 91,888,157 bp
  • A to T, chromosome 6 at 99,714,476 bp
  • T to A, chromosome 6 at 115,887,005 bp
  • T to C, chromosome 7 at 24,228,074 bp
  • A to G, chromosome 7 at 45,126,521 bp
  • A to G, chromosome 7 at 83,973,214 bp
  • A to T, chromosome 7 at 101,826,778 bp
  • T to G, chromosome 7 at 110,837,200 bp
  • C to T, chromosome 7 at 126,563,110 bp
  • A to T, chromosome 8 at 14,778,271 bp
  • G to A, chromosome 8 at 119,598,717 bp
  • T to A, chromosome 8 at 125,090,438 bp
  • T to C, chromosome 9 at 88,381,816 bp
  • G to A, chromosome 10 at 29,218,247 bp
  • C to A, chromosome 10 at 91,161,858 bp
  • T to A, chromosome 11 at 32,277,921 bp
  • T to G, chromosome 11 at 54,690,237 bp
  • A to G, chromosome 11 at 61,673,983 bp
  • A to T, chromosome 11 at 69,126,475 bp
  • T to G, chromosome 11 at 72,872,604 bp
  • T to A, chromosome 11 at 72,886,746 bp
  • G to A, chromosome 11 at 73,288,209 bp
  • T to A, chromosome 11 at 79,408,422 bp
  • A to G, chromosome 11 at 97,475,021 bp
  • T to C, chromosome 12 at 4,263,473 bp
  • G to T, chromosome 12 at 8,499,531 bp
  • A to T, chromosome 12 at 36,176,591 bp
  • G to T, chromosome 12 at 76,576,016 bp
  • G to A, chromosome 12 at 112,744,700 bp
  • A to T, chromosome 13 at 23,075,886 bp
  • C to A, chromosome 13 at 33,334,604 bp
  • A to G, chromosome 13 at 94,394,786 bp
  • T to A, chromosome 14 at 34,245,232 bp
  • C to A, chromosome 14 at 63,492,820 bp
  • G to T, chromosome 14 at 122,465,062 bp
  • C to T, chromosome 15 at 78,994,126 bp
  • C to A, chromosome 15 at 101,529,616 bp
  • T to C, chromosome 16 at 16,915,178 bp
  • T to C, chromosome 16 at 73,013,023 bp
  • G to A, chromosome 17 at 5,712,414 bp
  • A to T, chromosome 17 at 20,570,419 bp
  • A to G, chromosome 17 at 22,602,489 bp
  • C to A, chromosome 17 at 84,649,706 bp
  • T to A, chromosome 18 at 9,876,980 bp
  • T to A, chromosome 18 at 37,807,294 bp
  • G to T, chromosome 19 at 8,594,231 bp
  • G to A, chromosome 19 at 32,006,259 bp
  • A to T, chromosome 19 at 43,804,971 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8492 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067934-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.