Strain Name:
C57BL/6N-Ace2em1Mbp/Mmucd
Stock Number:
068346-UCD
Citation ID:
RRID:MMRRC_068346-UCD
Other Names:
ACE2 KI

Gene Information

Ace2em1Mbp
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; endonuclease-mediated mutation 1, Mouse Biology Program
Type: Allele
Species: Multi-species
Chromosome: X
Ace2
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Synonyms: 2010305L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 70008
Homologene: 41448
Genetic Alterations
Generation of a humanized ACE2 (ENSG00000130234.13) mouse line. Human DNA consisting of 5'UTR, CDS (exons 2-19) fused to mouse 3'UTR replaced the DNA region in mouse Ace2 gene stretching from the 5'UTR to the starting ATG in exon 2. The approach minimizes potential downstream splicing or RNP re-cleavage. CRISPR RNP was used to assist with homologous recombination using dsDNA repair in ES cells. The expression of the human CDS is controlled by the mouse regulatory systems (including 3’UTR) as the expression pattern is similar between species and especially high in the lungs.
ES Cell Line
JM8A3 derived from C57BL/6N
Phenotype
Pending analysis.

Note: Due to the use of JM8A3 embryonic stem cells, agouti coat color is possible.
Strain Development
CRISPR RNP was used to assist with homologous recombination using dsDNA repair template in JM8A3 ES cells derived from C57BL/6N. Following blastocyst injection, the chimeras were mated with C57BL/6NCrl to create germline heterozygotes. Long-range PCR products were fully sequenced; potential off-targets were absent. Subsequent backcross to C57BL/6NCrl.
Suggested Control Mice
C57BL/6NCrl or wild-type siblings
  • Immunology and Inflammation
Donor
Kent Lloyd, D.V.M., University of California, Davis.
Primary Reference
Pending publication

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu
Coat Color
Black or agouti
Eye
Black
MMRRC Breeding System
Backcross and sib-mating
Generation
N2 (C57BL/6NCrl), F1
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

A license from third party patent owner(s) may be required for for-profit entities to use mouse models derived from CRISPR-Cas9 technologies and it is the Users sole responsibility to determine whether such a license is necessary.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.